Production, Purification, and Characterization of Bacillibactin Siderophore of Bacillus subtilis and Its Application for Improvement in Plant Growth and Oil Content in Sesame

Nithyapriya, S.; Sundaram, Lalitha; Sayyed, R. Z.; Reddy, M. S.; Dailin, Daniel Joe; Enshasy, Hesham Ali El; Suriani, Ni Luh; Herlambang, Susila

doi:10.3390/su13105394

Cited by 109 publications

(71 citation statements)

References 67 publications

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“…In the past few decades, PGPR that belong to the species of Bacillus isolated from the rhizosphere and marine sources have been used for plant growth promotion. The Bacillus species are preferred for their use in agriculture due to their ability to produce bioactive metabolites and endospores to withstand biotic and abiotic stress conditions, which thereby promote plant growth and nutrient uptake [16][17][18][19]. In the present study, Bacillus spp., isolated from marine water and the rhizosphere, were evaluated for improvement in growth, yield, and iron content in sorghum under in vitro and in vivo conditions.…”

Section: Introductionmentioning

confidence: 88%

Co-Inoculation of Bacillus spp. for Growth Promotion and Iron Fortification in Sorghum

et al. 2021

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Seven Bacillus spp. isolated from the marine water and the rhizosphere of the medicinal plant Coscinium fenestratum were studied to produce plant growth promotion (PGP) traits invitro. Among the seven isolates, MMRH22 and RHPR20 produced copious amounts of PGP traits. Based on the 16S rRNA sequence, the two potent bacterial isolates, RHPR20 and MMRH22, were identified as Bacillus mojavensis and Bacillus cereus, respectively. A compatibility test between the isolates RHPR20 and MMRH22 revealed they are compatible and can be used as a consortium. Both isolates were evaluated for the plant growth promotion and the biofortification of sorghum under greenhouse conditions. Treatments included the application of MMRH22, RHPR20, their consortium (RHPR20 + MMRH22), and an uninoculated control. Inoculation with bacterial cultures resulted in a significant increase in the plant height; the number of leaves; the leaf area; the root, shoot, and leaf weight; and the yield of sorghum at 30 and 60 days after sowing (DAS). The scanning electron micrograph of the sorghum plant roots revealed extensive colonization in the plants treated with the bacterial cultures compared to the uninoculated control. The sorghum grains obtained after final harvest were analyzed for their nutrient content by ICP–OES. The biofortification in sorghum grains was varied and was found to enhance the iron content up to 97%. This study revealed that treatments with microbial consortia enhance plant growth, yield, and iron content, which could combat nutrient deficiencies in plants and humans.

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Section: Introductionmentioning

confidence: 88%

Co-Inoculation of Bacillus spp. for Growth Promotion and Iron Fortification in Sorghum

et al. 2021

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“…Bacillibactin is usually produced from Bacillus spp. which is an iron regulatory siderophore and exhibits antimicrobial activity [ 30 , 31 ]. A gene cluster also responsible for production of terpenes was found.…”

Section: Discussionmentioning

confidence: 99%

A Metagenomic Nanopore Sequence Analysis Combined with Conventional Screening and Spectroscopic Methods for Deciphering the Antimicrobial Metabolites Produced by Alcaligenes faecalis Soil Isolate MZ921504

Eltokhy

Saad²,

Eltayeb

et al. 2021

Antibiotics

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The continuous development of multidrug resistance pathogens with limited therapeutic options has become a great problem globally that impose sever health hazards. Accordingly, searching for of new antimicrobials became an urgent demand and great challenge. Soil significantly have been associated with several species that are antibiotic producers. In this study, combination of conventional screening methods with Liquid chromatography- Mass spectroscopy (LC/MS) and metagenomic nanopore sequence analysis have been conducted for the deciphering the active metabolites produced by soil isolate(s). Preliminary soil screening resulted in a Gram-negative isolate identified via 16S ribosomal RNA as Alcaligenes faecalis isolate MZ921504 with promising antimicrobial activities against wide range of MDR gram-positive and gram-negative pathogens. The LC/MS analysis of the metabolites of A. faecalis isolate MZ921504 confirmed the presence of ectoine, bacillibactin, quinolobactin and burkholderic acid. Metagenomics sequence analysis of the soil sample (NCBI GenBank accession PRJNA771993) revealed the presence of conserved biosynthetic gene clusters of ectoine, bacteriocin, bacillibactin, quinolobactin, terpene and burkholderic acid of A. faecalis. In conclusion, A. faecalis isolate MZ921504 is a promising source for antimicrobial metabolites. LC/MS spectral analysis and third generation sequencing tools followed by secondary metabolite gene clusters analysis are useful methods to predict the nature of the antimicrobial metabolites.

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“…When a transparent halo appears around the colonies, it indicates the ability to solubilize phosphate. The production of siderophore was uncovered using chrome azurol S (CAS) agar plates, and the change of color of surroundings of colonies from blue to orange denotes the presence of siderophores (Nithyapriya et al, 2021). The ability of R. nepotum to fix N was tested using a nitrogen-free media.…”

Section: Plant Growth-promoting Characteristicsmentioning

confidence: 99%

Biodegradation of Phenol by Bacillus simplex: Characterization and Kinetics Study

Magharbeh¹,

Khleifat²,

Al-kafaween³

et al. 2021

Appl Env Biotechnol

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Phenol is one of the main pollutants that have a serious impact on the environment and can even be very critical to human health. The biodegradation of phenol can be considered an increasingly important pollution control process. In this study, the degradation of phenol by Bacillus simplex was investigated for the first time under different growth conditions. Six different initial concentrations of phenol were used as the primary substrate. Culture conditions had an important effect on these cells' ability to biodegrade phenol. The best growth of this organism and its highest biodegradation level of phenol were noticed at pH 7, temperature 28 °C, and periods of 36 and 96 h, respectively. The GC-MS analysis of the bacterial culture sample revealed that further degradation of the catechol by 1,2-dioxygenase produce a cis, cis-mucconic acid via ortho-pathway and/or by 2,3-dioxygenase into 2-hydroxymucconic semialdehyde via meta-pathway. The highest biodegradation rate was perceived at 700 mg/L initial phenol concentration. Approximately 90% of the phenol (700 mg / L) was removed in less than 96 hours of incubation time. It was found that the Haldane model best fitted the relationship between the specific growth rate and the initial phenol concentration, whereas the phenol biodegradation profiles with time could be adequately described by the modified Gompertz model. The obtained parameters from the Haldane equation are: 1.05 h−1, 9.14 ppm, and 329 ppm for Haldane's maximum specific growth rate, the half-saturation coefficient, and the Haldane’s growth kinetics inhibition coefficient, respectively. The Haldane equation fitted the experimental data by minimizing the sum of squared error (SSR) to 1.36 X 10-3.

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Production, Purification, and Characterization of Bacillibactin Siderophore of Bacillus subtilis and Its Application for Improvement in Plant Growth and Oil Content in Sesame

Cited by 109 publications

References 67 publications

Co-Inoculation of Bacillus spp. for Growth Promotion and Iron Fortification in Sorghum

Co-Inoculation of Bacillus spp. for Growth Promotion and Iron Fortification in Sorghum

A Metagenomic Nanopore Sequence Analysis Combined with Conventional Screening and Spectroscopic Methods for Deciphering the Antimicrobial Metabolites Produced by Alcaligenes faecalis Soil Isolate MZ921504

Biodegradation of Phenol by Bacillus simplex: Characterization and Kinetics Study

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