Production, purification and characterisation of oligosaccharides from olive tree pruning autohydrolysis

Cara, Cristóbal; Ruiz, Encarnación; Carvalheiro, Florbela; Moura, Patrícia; Ballesteros, Ignacio; Castro, Eulógio; Gı́rio, Francisco M.

doi:10.1016/j.indcrop.2012.03.017

Cited by 71 publications

(33 citation statements)

References 34 publications

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“…However, most of the solubilised glucose did not have its origin in cellulose but on starch and glucosides present in the leaves [ 42 , 46 , 47 ]. This nonstructural glucose present in the extractives is released even at mild pretreatment conditions [ 39 , 44 ].…”

Section: Resultsmentioning

confidence: 99%

Endophytic Fungi as Pretreatment to Enhance Enzymatic Hydrolysis of Olive Tree Pruning

Martín‐Sampedro

López-Linares

Fillat

et al. 2017

BioMed Research International

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Olive tree pruning, as one of the most abundant lignocellulosic residues in Mediterranean countries, has been evaluated as a source of sugars for fuel and chemicals production. A mild acid pretreatment has been combined with a fungal pretreatment using either two endophytes (Ulocladium sp. and Hormonema sp.) or a saprophyte (Trametes sp. I-62). The use of endophytes is based on the important role that some of them play during the initial stages of wood decomposition. Without acid treatment, fungal pretreatment with Ulocladium sp. provided a nonsignificant enhancement of 4.6% in glucose digestibility, compared to control. When a mild acid hydrolysis was carried out after fungal pretreatments, significant increases in glucose digestibility from 4.9% to 12.0% (compared to control without fungi) were observed for all fungal pretreatments, with maximum values yielded by Hormonema sp. However, despite the observed digestibility boost, the total sugar yields (taking into account solid yield) were not significantly increased by the pretreatments. Nevertheless, based on these preliminary improvements in digestibility, this work proves the potential of endophytic fungi to boost the production of sugar from olive tree pruning, which would add an extra value to the bioeconomy of olive crops.

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Section: Resultsmentioning

confidence: 99%

Endophytic Fungi as Pretreatment to Enhance Enzymatic Hydrolysis of Olive Tree Pruning

Martín‐Sampedro

López-Linares

Fillat

et al. 2017

BioMed Research International

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“…In contrast, LS30 could remove all the formic acid, acetic acid, glycolic acid, and furfural, with a 92.0% xylo-oligosaccharides recovery. Cara et al (2012) reported that about 80% oligosaccharides yields were obtained after purification by preparative gel filtration chromatography of olive tree pruning hydrolysate. The low glucuronic acid removal rate of 8% may have resulted from the formation of glucuronolactone at the pH value of 4, from which it is difficult to obtain a reaction with an anion exchange resin (Canilha et al 2004).…”

Section: Comparison Of Different Purification Methodsmentioning

confidence: 99%

Xylo-oligosaccharides and Ethanol Production from Liquid Hot Water Hydrolysate of Sugarcane Bagasse

Zhuang

et al. 2014

BioRes

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aWith the objective of maximizing the use of liquid hot water hydrolysate of sugarcane bagasse, xylo-oligosaccharides and ethanol were respectively produced by the methods of purification and microbial fermentation. The processes of purification with activated charcoal, overliming, solvent extraction, vacuum evaporation, and use of an ion exchange resin were evaluated, and the results indicated that anion exchange chromatography performed well in terms of by-product removal. The recovery and purity of xylo-oligosaccharides reached 92.0% and 90.4%, respectively, using column chromatography with the resin LS30 at a flow rate of 2 mL/min at 25 C. The hydrolysate was used in ethanol fermentation with Pichia stipitis CBS6054 followed by the production of fermentable saccharides and detoxification. The highest ethanol concentration was 4.12 g/L with a theoretical yield of 47.9% for the hydrolysate after xylanase digestion and resin detoxification, similar to the data of the control experiment, which had an ethanol concentration of 4.64 g/L and a yield of 49.6%. However, the former had a higher ethanol productivity of 0.0860 g/(L•h), and the highest ethanol concentration appeared 12 to 24 h earlier compared to the control. This study suggests that combined generation of xylo-oligosaccharides and cellulosic ethanol could help maximize profits for a cane sugar factory.

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“…Initial experiments at same temperatures without any HCl in the reaction medium confirm the absence of any non-catalytic reaction (autohydrolysis), with no monomers detected by HPLC after 4 h at same reaction conditions. The sawdust autohydrolysis has been previously reported but at temperatures higher than 100°C, usually close to 200°C (Sidiras et al, 2011;Cara et al, 2012).…”

Section: Influence Of the Hydrolysis Temperature On The Products Distmentioning

confidence: 97%

Selective arabinose extraction from Pinus sp. sawdust by two-step soft acid hydrolysis

Bravo

Garcés

Faba

et al. 2017

Industrial Crops and Products

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Acid hydrolysis of Pinus sp. sawdust is optimized in this work in order to selectively recover arabinose from the hemicellulose fraction by an environmental-friendly process (reactions at atmospheric pressure and mild conditions and using dilute HCl as catalyst). The influence of temperature, HCl concentration and reaction time was studied in the first experiments considering one step hydrolysis. One step hydrolysis was studied at different conditions, being the best results obtained at 65°C for 18 h with 3% of HCl, with more than 3.8 g/L of arabinose in the liquid phase. This value corresponds to more than 47.5% of recovery of the arabinose present in the original sawdust and a selectivity of a 52% in the liquid phase (hydrolysis of other hemicellulosic sugars). In a second approach, a two steps process was considered in order to maximize the arabinose recovery. The optimum results were obtained combining a first cycle at 65°C with a second one at 80°C. More than 54% of the initial arabinose was recovered in the liquid phase as monomers. This percentage increases to 100% if the oligomers are also considering (no arabinose detected in the solid phase after the treatment). At these conditions, more than 56% of the global hemicellulose sugars were removed from the solid phase, without having a relevant degradation of glucose (pentose selectivity in liquid phase is higher than 88%, corresponding the resto mainly to hemicellulosic glucose).

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Production, purification and characterisation of oligosaccharides from olive tree pruning autohydrolysis

Cited by 71 publications

References 34 publications

Endophytic Fungi as Pretreatment to Enhance Enzymatic Hydrolysis of Olive Tree Pruning

Endophytic Fungi as Pretreatment to Enhance Enzymatic Hydrolysis of Olive Tree Pruning

Xylo-oligosaccharides and Ethanol Production from Liquid Hot Water Hydrolysate of Sugarcane Bagasse

Selective arabinose extraction from Pinus sp. sawdust by two-step soft acid hydrolysis

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