Production, partial characterization and antioxidant properties of exopolysaccharide α-d-glucan produced by Leuconostoc lactis KC117496 isolated from an idli batter

Saravanan, Chinnashanmugam; Kavitake, Digambar; Kandasamy, Sujatha; Devi, Palanisamy Bruntha; Shetty, Prathapkumar Halady

doi:10.1007/s13197-018-3469-3

Cited by 19 publications

(9 citation statements)

References 31 publications

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“…The water solubility of the EPSs was attributed to their absorptive structure which can hold water through hydrogen bonds (Yang et al 2019). The WSI range of bacterial EPSs extends from 14.2 to 92.15% (Saravanan et al 2019;Vinothini et al 2019), so with a WSI exceeding 85% Leptolyngbya sp., EPSs are considered among the most water-soluble ones.…”

Section: Discussionmentioning

confidence: 99%

Functional, rheological, and antioxidant properties of extracellular polymeric substances produced by a thermophilic cyanobacterium Leptolyngbya sp.

et al. 2022

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Extracellular polymeric substances (EPSs) produced by the filamentous cyanobacterium identified as Leptolyngbya sp. IkmLPT16 were isolated and characterized chemically, and their antioxidant, functional, and rheological properties were studied. The strain produces a significant amount of EPSs (2.15 g L −1 ) conjointly with a biomass production achieved at a maximum of 1.35 g L −1 after nine production days. Chemical analysis of EPSs revealed the presence of mannose (35%), arabinose (24%), glucose (15%), rhamnose (2%), and one uronic acid (8%). Fourier transformed infrared spectrum of EPSs further revealed the presence of νC-N groups indicating the presence of peptide moieties. Elemental analysis of EPSs showed the presence of sulfate groups (S = 0.59%) as inorganic substituents. Functional properties of Leptolyngbya EPSs were determined based on water holding capacity, oil holding capacity, foaming ability, and metal sorption ability. Experimental results showed high water holding capacity (119%), water solubility index (97.43%), and oil holding ability (87.52%), with a strong metal sorption ability and consequent foam stability (22%). The rheological properties of EPSs were comparable with commercial xanthan gum with higher resistance to Temperature. Leptolyngbya sp. EPSs displayed an effective antioxidant activity via directly scavenging free radicals particularly DDPH• (IC 50 = 4 mg. mL −1 against 10 mg. mL −1 for l-ascorbic acid) and •OH (IC 50 = 10 mg. mL −1 against 20 mg. mL −1 for l-ascorbic acid) and as an iron-chelating agent (IC 50 = 40 mg. mL −1 against 60 mg.mL −1 for EDTA). The outcomes of this study demonstrate the potential use of Leptolyngbya sp. EPSs in several food and pharmaceutical applications.

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Section: Discussionmentioning

confidence: 99%

Functional, rheological, and antioxidant properties of extracellular polymeric substances produced by a thermophilic cyanobacterium Leptolyngbya sp.

et al. 2022

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“…Glucan (500 μg/ ml) isolated from idli batter displayed significant antioxidant activity in DPPH (74%) and hydroxyl radical scavenging assays (97.8%). This ensures that the glucan has the promising future in food and pharmaceutical industry [98]. Two EPS fractions of acidic HePs rich in mannose and galactose produced by Weissella cibaria SJ14 showed strong antioxidant activity [99].…”

Section: Antioxidant Activitymentioning

confidence: 99%

Exopolysaccharides from probiotic bacteria and their health potential

Angelin

Kavitha

2020

International Journal of Biological Macromolecules

356

225

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Exopolysaccharides (EPS) are extracellular macromolecules excreted as tightly bound capsule or loosely attached slime layer in microorganisms. They play most prominent role against desiccation, phagocytosis, cell recognition, phage attack, antibiotics or toxic compounds and osmotic stress. In the last few decades, natural polymers have gained much attention among scientific communities owing to their therapeutic potential. In particular the EPS retrieved from probiotic bacteria with varied carbohydrate compositions possess a plenty of beneficial properties. Different probiotic microbes have unique behavior in expressing their capability to display significant health promoting characteristics in the form of polysaccharides. In this new era of alternative medicines, these polysaccharides are considered as substitutes for synthetic drugs. The EPS finds applications in various fields like textiles, cosmetics, bioremediation, food and therapeutics. The present review is focused on sources, chemical composition, biosynthetic pathways of EPS and their biological potential. More attention has been given to the scientific investigations on antimicrobial, antitumor, anti-biofilm, antiviral, anti-inflammatory and immunomodulatory activities.

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“…These are considered to be safer alternatives to the synthetic ones. In the recent years, many EPS from LAB have been studied for their antioxidant potential and prevention of oxidative damage 22 , 23 . Antioxidant potential and protective role of Weissella EPS have also been reported from a variety of EPS isolated from Weissella many strains like Weissella confusa EPSWWC, W. confusa OF126, W. cibaria GA44, W. cibaria YB-1, W. confusa W4 and W. cibaria SJ14 24 – 29 .…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress alleviating potential of galactan exopolysaccharide from Weissella confusa KR780676 in yeast model system

Kavitake

Veerabhadrappa

Sudharshan

et al. 2022

Sci Rep

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In the present study, galactan exopolysaccharide (EPS) from Weissella confusa KR780676 was evaluated for its potential to alleviate oxidative stress using in vitro assays and in vivo studies in Saccharomyces cerevisiae (wild type) and its antioxidant (sod1∆, sod2∆, tsa1∆, cta2∆ and ctt1∆), anti-apoptotic (pep4∆ and fis1∆) and anti-aging (sod2∆, tsa1∆ and ctt1∆)) isogenic gene deletion mutants. Galactan exhibited strong DPPH and nitric oxide scavenging activity with an IC50 value of 450 and 138 µg/mL respectively. In the yeast mutant model, oxidative stress generated by H2O2 was extensively scavenged by galactan in the medium as confirmed using spot assays followed by fluorescencent DCF-DA staining and microscopic studies. Galactan treatment resulted in reduction in the ROS generated in the yeast mutant cells as demonstrated by decreased fluorescence intensity. Furthermore, galactan exhibited protection against oxidative damage through H2O2 -induced apoptosis inhibition in the yeast mutant strains (pep4∆ and fis1∆) leading to increased survival rate by neutralizing the oxidative stress. In the chronological life span assay, WT cells treated with galactan EPS showed 8% increase in viability whereas sod2∆ mutant showed 10–15% increase indicating pronounced anti-aging effects. Galactan from W. confusa KR780676 has immense potential to be used as a natural antioxidant for nutraceutical, pharmaceutical and food technological applications. As per our knowledge, this is the first report on in-depth assessment of in vivo antioxidant properties of a bacterial EPS in a yeast deletion model system.

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Production, partial characterization and antioxidant properties of exopolysaccharide α-d-glucan produced by Leuconostoc lactis KC117496 isolated from an idli batter

Cited by 19 publications

References 31 publications

Functional, rheological, and antioxidant properties of extracellular polymeric substances produced by a thermophilic cyanobacterium Leptolyngbya sp.

Functional, rheological, and antioxidant properties of extracellular polymeric substances produced by a thermophilic cyanobacterium Leptolyngbya sp.

Exopolysaccharides from probiotic bacteria and their health potential

Oxidative stress alleviating potential of galactan exopolysaccharide from Weissella confusa KR780676 in yeast model system

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