Production, Optimization, Purification, Testing Anti-Microbial and Dye Decolorizing Properties of Penicillin G Acylase from Penicillium chrysogenum.

Mahendiran, Balaji; Duraisamy, Vigneshwaran; Malarselvi,; Mohapatra, Anannya

doi:10.9790/3008-0735765

Cited by 1 publication

(1 citation statement)

References 9 publications

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“…In comparison to the SPG isolates, others found that the closely related species of P. chrysogenum and P. brevicompactum from continental environments grew in the range of 5 to 37 • C, and optimally at 25 to 30 • C (Pitt, 1973;Magan and Lacey, 1984;Sautour et al, 2001;Mahendiran et al, 2013). Additionally, fungi from shallow marine sediments were found to also be mesophilic and psychrotolerant (Singh et al, 2010).…”

Section: Identification and Characterization Of Spg Fungimentioning

confidence: 99%

Redefining the Subsurface Biosphere: Characterization of Fungi Isolated From Energy-Limited Marine Deep Subsurface Sediment

Reese¹,

Sobol²,

Bowles³

et al. 2021

Front. Fungal Biol.

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The characterization of metabolically active fungal isolates within the deep marine subsurface will alter current ecosystem models and living biomass estimates that are limited to bacterial and archaeal populations. Although marine fungi have been studied for over fifty years, a detailed description of fungal populations within the deep subsurface is lacking. Fungi possess metabolic pathways capable of utilizing previously considered non-bioavailable energy reserves. Therefore, metabolically active fungi would occupy a unique niche within subsurface ecosystems, with the potential to provide an organic carbon source for heterotrophic prokaryotic populations from the transformation of non-bioavailable energy into substrates, as well as from the fungal necromass itself. These organic carbon sources are not currently being considered in subsurface energy budgets. Sediments from South Pacific Gyre subsurface, one of the most energy-limited environments on Earth, were collected during the Integrated Ocean Drilling Program Expedition 329. Anoxic and oxic sediment slurry enrichments using fresh sediment were used to isolate multiple fungal strains in media types that varied in organic carbon substrates and concentration. Metabolically active and dormant fungal populations were also determined from nucleic acids extracted from in situ cryopreserved South Pacific Gyre sediments. For further characterization of physical growth parameters, two isolates were chosen based on their representation of the whole South Pacific Gyre fungal community. Results from this study show that fungi have adapted to be metabolically active and key community members in South Pacific Gyre sediments and potentially within global biogeochemical cycles.

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