Production of β-ketoadipic acid from glucose in Pseudomonas putida KT2440 for use in performance-advantaged nylons

Mixed plastics waste valorization through tandem chemical oxidation and biological funneling

“…The autoxidation products are appealing, however, because they have enhanced water solubility that make them advantaged feedstocks for biological funneling, wherein an engineered microbe converts diverse chemicals to a single product ( 20 ). To pursue this opportunity, we engineered two strains of Pseudomonas putida ( 21 , 22 ): first, to convert acetate, C 4 to C 17 dicarboxylates, benzoate, and terephthalate to polyhydroxyalkanoates, a natural polyester with growing industrial applications ( 23 ), and second, to use acetate and dicarboxylates for growth while converting benzoate and terephthalate to β-ketoadipate, a monomer for performance-advantaged polymers ( 24 , 25 ) (Fig. 3).…”

Biological conversion of cyclic ketones from catalytic fast pyrolysis with Pseudomonas putida KT2440

“…Quantitation of methanol was conducted using chromatographic conditions utilizing a Aminex HPX-87H column (Bio-Rad Laboratories), as described in Rorrer et al , 2022 35 with the addition of methanol as an analyte. A minimum of 6 calibration levels were used for quantification with a linear range 0.1 g L −1 to 10 g L −1 .…”

Biochemical Conversion of Lignocellulosic Biomass to Hydrocarbon Fuels and Products: 2022 State of Technology and Future Research

“…Following a pivot in 2021, experimental work in 2022 continued to focus on bioconversion of lignin monomers to BKA, building on prior recent experimental and TEA modeling work demonstrating superior fermentation performance and economics for BKA production from Pseudomonas putida than adipic acid, with concomitantly superior properties as a performanceadvantaged bioproduct [16,17]. On the latter point, the addition of the β-ketone into the adipic acid molecule structure was found to enable superior thermal properties for nylon-6,6, a key derivative product, when synthesized from BKA in place of adipic acid, while enabling favorable carbon intensity compared to traditional adipic acid production [16,17]. The BKA fermentation step itself was maintained identical to prior TEA modeling details described for muconic acid fermentation, based on fed-batch fermentation of aromatic lignin monomers available in the hydrolysate substrate [2].…”