Production of zearalenone, moniliformin and trichothecenes in intact sugar beets under laboratory conditions

Bosch, U.; Mirocha, C. J.; Wen, Yechun

doi:10.1007/bf00448815

Cited by 12 publications

(4 citation statements)

References 25 publications

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“…He also found that the wheat samples were negative, half of the wheat bran was positive with an average contamination [24]. Bosch et al [5] reported that E graminearum and E equiseti originally obtained from corn and corn-based feedstuff were highly ZEN and trichothecence-producing. ZEN production on rice reached…”

Section: Natural Occurrence Of Zen In Corn Rice and Wheat In Egyptmentioning

confidence: 99%

Zearalenone: Incidence, toxigenic fungi and chemical decontamination in Egyptian cereals

Alla

1997

Nahrung

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An investigation was made for the occurrence of Zearalenone (ZEN) and Zearalenone producing fungi in cereals (corn, 50 samples; rice, 45 smples; wheat, 40 samples) collected from Egypt. ZEN was detected in 15 of 50 corn samples with an average concentration of 22.3 ppb. The incidence of ZEN in rice samples was 8.9% (4 samples of 4 3 , and the average was 15.5 pph. Out of 40 wheat samples 5 samples were contaminated with ZEN (12.5%) with an average of 8.8 ppb. Seventy-nine Fusarium strains belonging to 9 different species were isolated from Egyptian cereals, and when tested for ZEN production only 26 isolates were Zearalenone producer. Efficiency of H202 for destruction of ZEN in contaminated com was studied at different concentration (3, 5 and 10%). The results revealed that per cent of disappearance of ZEN was found to be dependent upon the concentration of H20,, temperature and period of exposure. The highest per cent of degradation was 83.9%, with 10% H20, at 80°C for 16 h, followed by 75% at the same conditions for 8 h, while the lowest one obtained by 3% H202 at 50°C for 2 h. * 2°C. Cultures were then inoculated (1 cm diameter of agar from PDA plates of the Fusarium spp.) on autoclaved rice (100 g) with 30% moisture content and were placed in 1-1-Erlenmeyer flasks. The cultures were incubated for 4 weeks at 20°C.

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Section: Natural Occurrence Of Zen In Corn Rice and Wheat In Egyptmentioning

confidence: 99%

Zearalenone: Incidence, toxigenic fungi and chemical decontamination in Egyptian cereals

Alla

1997

Nahrung

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“…They are usually mixtures of methanol±1% aqueous NaCl, whose ratios vary from 55:45 (Bottalico et al 1983 to 60:40 (Tanaka et al 1995, JimeÂ nez et al 1996, JimeÂ nez and Mateo 1997, Bresler et al 1998. Other procedures use acetonitrile±methanol±water (80:5:15) (Di Menna et al 1991, Lauren andAgnew 1991), acetonitrile±water (84:16) (Stratton et al 1993, Trigo-Stockli et al 1996), or chloroform±water (250:20) (CuniOE 1995.…”

Section: Introductionmentioning

confidence: 98%

“…Other procedures use acetonitrile±methanol±water (80:5:15) (Di Menna et al 1991, Lauren andAgnew 1991), acetonitrile±water (84:16) (Stratton et al 1993, Trigo-Stockli et al 1996), or chloroform±water (250:20) (CuniOE 1995. The clean-up of extracts before chromatographic separation has been done using solid-phase extraction (SPE) cartridges such as C-18 (Bottalico et al 1983, 1985, Windels et al 1989, Lauren and Agnew 1991, Seidel et al 1993, JimeÂ nez and Mateo 1997, silica (Howell andTaylor 1981, Seidel et al 1993) and Florisil (Tanaka et al 1985, Kim et al 1993, Seidel et al 1993. The liquid±liquid extraction between chloroform and alkaline aqueous solution used in the actual AOAC International O cial Method of Analysis for ZEA uses large volumes of chloroform is time-consuming and cannot be automated easily (CuniOE1995, Ware et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Comparison of extraction and clean-up procedures for analysis of zearalenone in corn, rice and wheat grains by high-performance liquid chromatography with photodiode array and fluorescence detection

Llorens

Mateo

et al. 2002

Food Additives and Contaminants

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The aim of this work was the optimization of some procedures usually used in the analysis of zearalenone (ZEA) in corn and other cereals by high-performance liquid chromatography (HPLC) with photodiode array and/or fluorescence detection. The comparison of five extraction solvents is presented. Three solid-phase extraction cartridges (C-18, silica, Florisil) and immuno-affinity columns were also compared to obtain the best recovery of the mycotoxin with the minimal presence of co-extractives in the chromatograms. Mixtures of methanol-1% aqueous NaCl (80.20 or 60:40 v/v) were the best extraction solvents. Florisil provided higher recovery of ZEA than C-18, and silica proved unsuitable. The immuno-affinity column was very efficient in cleaning the extracts, but its sample capacity was lower than that of SPE columns due to saturation. The mobile phase (methanol-water 80:20 v/v) gave a low retention time for ZEA (approximately 5 min), high sensitivity and acceptable separation between this mycotoxin and alpha-zearalenol. The optimized protocol is straightforward, provides high ZEA recoveries in spiked corn (mean 102.4%), has an acceptable sensitivity and has a lack of interference with fluorescence detection (detection limit 4 ng ZEA g(-1) corn). The photodiode array detector was useful, except at very low ZEA levels, to confirm the identity of the mycotoxin. The method was applied to search for ZEA accumulation in corn, wheat and rice grains inoculated with selected strains of Fusarium graminearum, F. oxysporum and method was applied to search for ZEA accumulation in corn, wheat and rice grains inoculated with selected strains of Fusarium graminearum, F. oxysporum and F. culmorum.

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“…F. poae produces high levels of nivalenol, T-2, and HT-2 on cereals and lower levels of diacetoxyscirpenol (DAS). It also produces fusarenone-X and monoacetoxyscirpenol (Bosch et al, 1992;Farber and Sanders, 1986;Gilbert et al, 1992;Marasas et al, 1984;Pettersson and Olvaang, 1995).…”

mentioning

confidence: 99%

Nitrate Nonutilizing Mutants and Vegetative Compatibility Groups inFusarium poae

Liu

Sundheim

1996

Fungal Genetics and Biology

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Production of zearalenone, moniliformin and trichothecenes in intact sugar beets under laboratory conditions

Cited by 12 publications

References 25 publications

Zearalenone: Incidence, toxigenic fungi and chemical decontamination in Egyptian cereals

Zearalenone: Incidence, toxigenic fungi and chemical decontamination in Egyptian cereals

Comparison of extraction and clean-up procedures for analysis of zearalenone in corn, rice and wheat grains by high-performance liquid chromatography with photodiode array and fluorescence detection

Nitrate Nonutilizing Mutants and Vegetative Compatibility Groups inFusarium poae

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