Production of Xylitol from Oil Palm Empty Friuts Bunch: A Case Study on Bioefinery Concept

Kresnowati, Made Tri Ari Penia; Mardawati, Efri; Setiadi, Tjandra

doi:10.5539/mas.v9n7p206

Cited by 30 publications

(24 citation statements)

References 37 publications

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“…Optimization of xylose concentration model is represented by the following scheme: Higher concentration of substrate for hydrolysis will make the process of hemicellulose conversion to xylose require longer time. When all xylan groups in hemicellulose hydrolyzed become xylose monomers and the process continues, xyloses produced will react with acid to become decomposition products such as acetic acid and furfural [13]. Increasing acid concentration to accelerate hydrolysis time is ineffective.…”

Section: I) Single Objective Optimization A) the Response Of Xylose Cmentioning

confidence: 99%

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2020

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Section: I) Single Objective Optimization A) the Response Of Xylose Cmentioning

confidence: 99%

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“…The raw materials are washed with clean water then followed by drying using sun and oven at 105°C overnight. Dry EFB was ground and was sieved using Tyler sieve 20 mesh and 60 mesh [24].…”

Section: A Preparation Of Raw Materialsmentioning

confidence: 99%

Fungal Production of Xylanase from Oil Palm Empty Fruit Bunches via Solid State Cultivation

Mardawati¹,

Kresnowati

Purwadi

et al. 2018

Int. J. Adv. Sci. Eng. Inf. Technol.

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The abundance of Oil Palm Empty Fruit Bunches (EFB), the lignocellulosic biomass waste from Crude Palm Oil (CPO) industries, offers a potential for its application as raw material for biorefinery. Its xylan content in particular, suggests that EFB can be used as raw material for xylanase production. This article studies xylanase production by fungal Trichoderma viride ITB CC L67, Aspergillus niger ITB CC L51, and Penicillium sp. ITB CC L96 using EFB as the substrate in solid state fermentation. The aims of this research are to evaluate the incubation time to produce the highest xylanase activity, to explore the effect of particle size of EFB on xylanase activity and to determine the optimal operation condition of each significant variable, including temperature and substrate ratio (g EFB in mL liquid medium) using Response Surface Methodology (RSM). The method of Central Composite Design (CCD) is employed to investigate the individual crucial component of the operation condition, which significantly affected the enzyme activity. The incubation time of T. viride, A. niger, and Penicillium sp. were observed to produce xylanase. The result show that the xylanase activity is increase in the early phase of incubation at 0-36 hours, and remained constant afterward. At the time of 36 hours, the activity of xylanase produced by T. viride, A. niger and Penicillium sp. were subsequently 815 U/mL, 624 U/mL and 789 U/mL or equal to 8,146 U/g, 6,243 U/g and 7,892 U/g dry substrate. The particle size of the EFB is affect the growth and hence affects on xylanase production. It shows that the smaller size of EFB resulted in better fungal growth, which was indicated by higher biomass dry weight and higher protein content, as well as higher xylanase activity. The optimum conditions obtained xylanase activity is 740.6 U/mL or 5,095.5 U/g substrate, produced by T.viride ITB CC L.67 at 32.8oC and substrate ratio of 0.63.

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“…The composition of lignocellulose in OPEFB, varies greatly, depending on the genetics type and environment in which the oil palm is grown (Kresnowati, Mardawati and Setiadi, 2015). Cellulose, hemicellulose, and lignin are mainly compound of lignocelulosic material.…”

Section: Opefbmentioning

confidence: 99%

Comparative Study of Glucose and Xylose Production in Enzymatic Hydrolysis Result by Batch and Fed Batch Method

Yudiastuti¹,

Mardawati²,

Kresnowati³

et al. 2018

Teknotan

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Xylose is a five carbon chain monosaccharide that can be used as a substrate in the fermentation of low-calorie xylitol. While glucose is a six carbon chain monosaccharide that can be processed into glucose syrup as a more stable natural sweetener in the food processing industry. Xylose is xylan monomer produced from hydrolysis of hemicellulose from plants or biomass woody waste. While glucose is the result which is also produced in xylan hydrolysis by xylanase enzymatic hydrolysys. In the search for the source of hemicellulose, oil palm empty fruit bunch (OPEFB) as a solid waste from crude palm oil processing is a source that can be utilized in this research. OPEFB waste is abundant by the increasing world demand for vegetable oil. Considering the OPEFB enzymatic hydrolysis was a hetrogenous solid-liquid reaction, fed batch feeding of substrate was thought to improve the reaction performance. Hydrolysis experiments were performed at temperature 500C, pH 5 and for 96 hours hydrolysys time with substrate concentration at 15% and enzyme dose of 1%. The result showed that the feed-batch hydrolysis configuration provided higher yield of xylose and glucose than batch configuration but unsignificant.Keywords : Enzymatic Hydrolysis, Fed-batch, glucose, Xylan, Xylanase, Xylose.

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Production of Xylitol from Oil Palm Empty Friuts Bunch: A Case Study on Bioefinery Concept

Cited by 30 publications

References 37 publications

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Untitled

Fungal Production of Xylanase from Oil Palm Empty Fruit Bunches via Solid State Cultivation

Comparative Study of Glucose and Xylose Production in Enzymatic Hydrolysis Result by Batch and Fed Batch Method

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