Production of xylitol by metabolically engineered strains of Bacillus subtilis

Povelainen, Mira; Miasnikov, Andrei N.

doi:10.1016/j.jbiotec.2006.09.008

Cited by 36 publications

(13 citation statements)

References 9 publications

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“…In comparison with that of organisms that naturally produce 5-carbon sugars or those that are improved by classical mutagenesis (1,4,5,12,36) or by the previously reported processes for xylitol production from D-glucose (19,21,23), the amounts of 5-carbon sugars and sugar alcohols produced by the recombinant S. cerevisiae strains of this study were low. One very likely reason is the low capacity of the PPP in S. cerevisiae; less than 4% of D-glucose is channeled through this pathway during respirofermentative metabolism (6,17).…”

Section: Discussioncontrasting

confidence: 65%

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Metabolic Engineering of Saccharomyces cerevisiae for Conversion of d -Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols

Toivari

Ruohonen

Miasnikov³

et al. 2007

Appl Environ Microbiol

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Recombinant Saccharomyces cerevisiae strains that produce the sugar alcohols xylitol and ribitol and the pentose sugar D-ribose from D-glucose in a single fermentation step are described. A transketolase-deficient S. cerevisiae strain accumulated D-xylulose 5-phosphate intracellularly and released ribitol and pentose sugars (D-ribose, Dribulose, and D-xylulose) into the growth medium. Expression of the xylitol dehydrogenase-encoding gene XYL2 of Pichia stipitis in the transketolase-deficient strain resulted in an 8.5-fold enhancement of the total amount of the excreted sugar alcohols ribitol and xylitol. The additional introduction of the 2-deoxy-glucose 6-phosphate phosphatase-encoding gene DOG1 into the transketolase-deficient strain expressing the XYL2 gene resulted in a further 1.6-fold increase in ribitol production. Finally, deletion of the endogenous xylulokinase-encoding gene XKS1 was necessary to increase the amount of xylitol to 50% of the 5-carbon sugar alcohols excreted.

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Section: Discussioncontrasting

confidence: 65%

“…In addition, they require a high yield at each individual step to make the overall process feasible. Recently, a pentulose-producing B. subtilis strain was modified to produce xylitol from D-glucose with a yield of around 20% (wt/wt) by expressing a xylitol-phosphate dehydrogenase-encoding gene in the host strain (23).…”

mentioning

confidence: 99%

Metabolic Engineering of Saccharomyces cerevisiae for Conversion of d -Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols

Toivari

Ruohonen

Miasnikov³

et al. 2007

Appl Environ Microbiol

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“…During the past decade many studies have been carried out aiming at improving the performance of the microbial process. The strategies employed include fedbatch fermentations (Vandeska et al 1996;Rodrigues et al 1998), cell-recycling (Bae et al 2004), cell immobilization (Yahashi et al 1996;Carvahlo et al 2005;Sarrouh et al 2007) and metabolic engineering of the microorganisms (Roca et al 1996;Chung et al 2002;Jeffries and Jin 2004;Povelainen and Miasnikov 2007).…”

Section: Introductionmentioning

confidence: 99%

Hydrogels based on u.v.-crosslinked poly(ethylene oxide) – matrices for immobilization of Candida boidinii cells for xylitol production

Winkelhausen

Jovanovic-Malinovska

Kuzmanova

et al. 2008

World J Microbiol Biotechnol

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Hydrogels based on high molecular weight poly(ethylene oxide) were synthesized by u.v.-irradiation of aqueous solutions in presence of the photoinitiator, (4-benzoylbenzyl)trimethylammonium chloride and different crosslinkers, poly(ethylene glycol), diacrylates and N,N 0 -methylenebisacrylamide. Candida boidinii cells were immobilized in these hydrogels and the gels were characterized in regards to gel fraction yield, degree of equilibrium swelling, shear storage and loss moduli. In addition, the number average molecular weight between crosslinks and the mesh size were estimated. The incorporated yeast cells considerably affected the viscoelastic properties of the gels. Immobilized C. boidinii cells were used for conversion of xylose to xylitol. Of the immobilized systems tested, only the system with poly(ethylene oxide) crosslinked with N,N 0 -methylenebisacrylamide exhibited xylitol production. The operational stability of this system was evaluated by seven repeated-batch runs performed in Erlenmeyer flasks in duration of 55 days. The progressive improvement of xylose consumption, up to 73.5%, stopped in the fifth cycle, after which it dropped to 42.7%. Although xylitol concentration never reached more than 4.2 g l -1 , xylitol was produced in each of the seven cycles. The cell leakage of 1.8 g l -1 during the first 45 days, indicated very good stability of the system.

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“…Xylitol can be synthesized by hydrogenation of xylose (Zakaria 2001). Xylitol also can be produced from D-glucose by three steps (Povelainen and Miasnikov 2006). Xylitol production from yeast is an alternative to chemical studies (Lachke and Jeffries 1986).…”

Section: Synthesismentioning

confidence: 99%

Artificial sweeteners – a review

2011

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Now a days sugar free food are very much popular because of their less calorie content. So food industry uses various artificial sweeteners which are low in calorie content instead of high calorie sugar. U.S. Food and Drug Administration has approved aspartame, acesulfame-k, neotame, cyclamate and alitame for use as per acceptable daily intake (ADI) value. But till date, breakdown products of these sweeteners have controversial health and metabolic effects. On the other hand, rare sugars are monosaccharides and have no known health effects because it does not metabolize in our body, but shows same sweet taste and bulk property as sugar. Rare sugars have no such ADI value and are mainly produced by using bioreactor and so inspite of high demand, rare sugars cannot be produced in the desired quantities.

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Production of xylitol by metabolically engineered strains of Bacillus subtilis

Cited by 36 publications

References 9 publications

Metabolic Engineering of Saccharomyces cerevisiae for Conversion of d -Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols

Metabolic Engineering of Saccharomyces cerevisiae for Conversion of d -Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols

Hydrogels based on u.v.-crosslinked poly(ethylene oxide) – matrices for immobilization of Candida boidinii cells for xylitol production

Artificial sweeteners – a review

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