Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selection

Wiśniewska, Halina; Majka, Maciej; Kwiatek, Michał; Gawłowska, Magdalena; Surma, M.; Adamski, T.; Kaczmarek, Z.; Drzazga, T.; Ługowska, Bogusława; Korbas, M.; Belter, Jolanta

doi:10.1016/j.ejbt.2018.10.003

Cited by 8 publications

(5 citation statements)

References 23 publications

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“…Homozygous plants were analyzed for the presence of the Pch1 gene along with some markers associated with that gene. The eyespot resistance gene Pch1 was detected in 65 doubled haploid lines of winter wheat, in the second year of the study field trials also confirmed the incorporation of the gene (Wiśniewska et al, 2019). In another study, DH lines of winter barley developed through androgenesis showed enhanced tolerance to drought and cold stress than their parental genotypes.…”

Section: Figuresupporting

confidence: 61%

Smart breeding approaches in post-genomics era for developing climate-resilient food crops

et al. 2022

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Section: Figuresupporting

confidence: 61%

Smart breeding approaches in post-genomics era for developing climate-resilient food crops

et al. 2022

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“…Currently, two main techniques are used: (a) doubled haploids (DHs) and (b) the fast generation cycle system (FGCS) [3]. Of these options, doubled haploids are the most widely used in different agricultural crops due to their efficiency in obtaining pure lines in crops such as Oryza sativa L. [5,6], Zea mays L. [7][8][9] and Triticum aestivum L. [10,11].…”

Section: Introductionmentioning

confidence: 99%

Haploid Induction in Tomato (Solanum lycopersicum L.) via Gynogenesis

Marín-Montes

Rodríguez-Pérez

Robledo-Paz

et al. 2022

Plants

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The generation of new hybrid varieties of tomato (Solanum lycopersicum L.) is the most widely used breeding method for this species and requires at least seven self-fertilization cycles to generate stable parent lines. The development of doubled haploids aims at obtaining completely homozygous lines in a single generation, although, to date, routine commercial application has not been possible in this species. In contrast, obtaining doubled haploid lines via gynogenesis has been successfully implemented in recalcitrant crops such as melon, cucumber, pumpkin, loquat and walnut. This review provides an overview of the requirements and advantages of gynogenesis as an inducer of haploidy in different agricultural crops, with the purpose of assessing the potential for its application in tomato breeding. Successful cases of gynogenesis variants involving in vitro culture of unfertilized ovules, use of 60Co-irradiated pollen, in vivo haploid inducers and wide hybridization are presented, suggesting that these methodologies could be implemented in tomato breeding programs to obtain doubled haploids.

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“…In spite of the logical correctness of this prediction, since our present knowledge on molecular interactions in Black pepper -P. capsici pathosystem is limited (Umadevi and Anandaraj, 2017) and moreover, the effect of ploidy is system speci c, development of dihaploids (DH) of 'PAJ' and comparing its resistance response with 'PAJ' is essential for a clear understanding on the performance of a dihaploid of 'PAJ' as regards to its resistance response for the pathogen. Wiśniewska et al (2019) have reported production of wheat-doubled haploids, which are more resistant to eyespot disease supported by marker-assisted selection. The present nding of remarkably high resistance response exhibited by the haploid genotype of Black pepper 'PAJ' compared to its diploid progenitor 'PMM' also facilitates comparative analysis of gene action pertaining to resistance for P. capsici, operating in these two ploidy systems of Black pepper.…”

Section: Resultsmentioning

confidence: 99%

Significance of ploidy on par with genotypic specificity in host resistance for Phytophthora in Black pepper (Piper nigrum L.)

Jacob,

Davis,

Jabbar

2024

Preprint

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We estimated Disease Severity Indices (DSI) of 22 genotypes of Black pepper by subjecting them to screening against Phytophthora capsici for assessing the influence of ploidy as well as genotype in host resistance. The DSI of the genotypes showed significant variation and established the existence of genotypic specificity in host resistance in Black pepper. ‘PAJ’, a haploid genotype of the crop, exhibited the highest resistance to the pathogen, as evident by its lowest DSI (33.33 %), and rated it as ‘moderately resistant’. Its diploid progenitor ‘PMM’ and all the other diploid genotypes were rated as ‘susceptible’, since their DSI were > 40 %. ‘PAJ’ is genetically a product of haplodisation of ‘PMM’, and it showed significantly higher resistance than ‘PMM’ for the pathogen. Hence, it is inferred that ploidy has remarkable influence in Black pepper – Phytophthorapathosystem. This study is insightful on the possible genomic interventions for augmenting the inherent degree of resistance exhibited by the genotypes of the crop for the pathogen. Therefore, outcome of this study is a breakthrough, which can transform the present-day haphazard, less efficient approach in resistance breeding in Black pepper for Phytophthora foot rot to one which is systematic and efficient.

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Production of wheat-doubled haploids resistant to eyespot supported by marker-assisted selection

Cited by 8 publications

References 23 publications

Smart breeding approaches in post-genomics era for developing climate-resilient food crops

Smart breeding approaches in post-genomics era for developing climate-resilient food crops

Haploid Induction in Tomato (Solanum lycopersicum L.) via Gynogenesis

Significance of ploidy on par with genotypic specificity in host resistance for Phytophthora in Black pepper (Piper nigrum L.)

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