Production of volatile organic compounds by Aureobasidium pullulans as a potential mechanism of action against postharvest fruit pathogens

Francesco, Alessandra Di; Ugolini, Luisa; Lazzeri, Luca; Mari, Marta

doi:10.1016/j.biocontrol.2014.10.004

Cited by 197 publications

(134 citation statements)

References 35 publications

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“…The main mechanism of action is based on competition with the pathogen for nutrients at the infection site, although hydrolytic enzymes are also produced (Castoria et al, 2001;Di Francesco et al, 2015a). It was recently demonstrated that A. pullulans produces volatile organic compounds that can prevent the germination of conidia of several pathogens, including those of B. cinerea (Di Francesco et al, 2015b), making the mechanism of action of this biocontrol agent more complex than previously thought or determined (Spadaro and Droby, 2016).…”

Section: Introductionmentioning

confidence: 99%

Combining biocontrol agents with different mechanisms of action in a strategy to control Botrytis cinerea on grapevine

et al. 2017

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Section: Introductionmentioning

confidence: 99%

Combining biocontrol agents with different mechanisms of action in a strategy to control Botrytis cinerea on grapevine

et al. 2017

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“…Such a system allows variations in VOCs production by antagonists and the influence of target pathogens to be monitored. Previous studies manually extracted VOCs from a double Petri dish system 11,16 without incorporation of suitable internal standards (IS). Thus, studies on the efficacy of mixtures of microbial VOCs have based their findings on the proportions of relative peak areas, derived from GC-MS chromatograms 17,18 .…”

mentioning

confidence: 99%

“…The antifungal activity of VOCs has been suggested to be synergistic or additive rather than a sole inhibitory action 11,24 . An inability to mimic the antagonists' antimicrobial activity by VOCs when applied individually 24 and production of VOCs at very low concentrations by antagonists 25 suggest that a synergistic or additive effect may occur.…”

mentioning

confidence: 99%

Aureobasidium pullulans volatilome identified by a novel, quantitative approach employing SPME-GC-MS, suppressed Botrytis cinerea and Alternaria alternata in vitro

Don

Schmidtke

Gambetta

et al. 2020

Sci Rep

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Volatile organic compounds (VOCs) produced by Aureobasidium pullulans were investigated for antagonistic actions against Alternaria alternata and Botrytis cinerea. Conidia germination and colony growth of these two phytopathogens were suppressed by A. pullulans Vocs. A novel experimental setup was devised to directly extract VOCs using solid-phase microextraction-gas chromatographymass spectrometry (SPME-GC-MS) from antagonist-pathogen culture headspace. The proposed system is a robust method to quantify microbial VOCs using an internal standard. Multivariate curve resolutionalternating least squares deconvolution of SPME-GC-MS spectra identified fourteen A. pullulans Vocs. 3-Methyl-1-hexanol, acetone, 2-heptanone, ethyl butyrate, 3-methylbutyl acetate and 2-methylpropyl acetate were newly identified in A. pullulans headspace. Partial least squares discriminant analysis models with variable importance in projection and selectivity ratio identified four VOCs (ethanol, 2-methyl-1-propanol, 3-methyl-1-butanol and 2-phenylethanol), with high explanatory power for discrimination between A. pullulans and pathogen. The antifungal activity and synergistic interactions of the four VOCs were evaluated using a Box-Behnken design with response surface modelling. Ethanol and 2-phenylethanol are the key inhibitory A. pullulans VOCs against both B. cinerea and A. alternata. Our findings introduce a novel, robust, quantitative approach for microbial VOCs analyses and give insights into the potential use of A. pullulans Vocs to control B. cinerea and A. alternata.Microbial antagonists have been widely explored as more environmentally friendly disease management alternatives to reduce the excessive use of synthetic fungicides 1,2 . Among a wide array of mechanisms exploited by microbial antagonists, the production of volatile organic compounds (VOCs) with antimicrobial properties 3 has become popular in the recent research due to their low risk of toxic residues, biodegradability 4 and enormous spectrum of activity regardless of a physical contact of the commodity 5 .Aureobasidium pullulans is a yeast-like saprophytic fungus, naturally inhabiting plant and fruit surfaces 6 . This yeast is a well-known biocontrol agent against a range of pathogenic fungi 7,8 and strains of the organism have been formulated as commercial preparations for the practical management of fungal phytopathogens of a range of horticultural crops 9 . Production of antifungal VOCs has been recently identified as a potential mode of action for its biocontrol 10,11 . One of these studies has confirmed the identity of its four VOCs, 2-methyl-1-butanol, 3-methyl-1-butanol, 2-methyl-1-propanol and 2-phenylethanol 11 . These compounds were shown to suppress conidia germination and mycelium growth of Botrytis cinerea, Colletotrichum acutatum, Penicillium expansum, Penicillium digitatum and Penicillium italicum under both in vitro and in vivo conditions 11 . A similar study identified over 20 VOCs from A. pullulans isolated from grapevine 12 . However, none of these c...

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“…In-vitro volatile antimicrobial activity was assessed using a plate-toplate method as described [27,40]. Bacterial cells grown on KB agar at 24°C for 24 h were used for antifungal activity assays.…”

Section: Volatile Antimicrobial Assaysmentioning

confidence: 99%

“…Enterobacter cloacae has been shown to inhibit Pythium ultimum sporangium germination and damping-off by competing for fatty acids [25]. Biological control using volatile compounds-producing microorganisms has been reported with increasing success on the various fruit crops and diseases [26][27][28][29][30][31][32][33][34]. However, little is known about volatile compounds produced by E. cloacae.…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Penicillium digitatum and Citrus Green Mold by Volatile Compounds Produced by Enterobacter cloacae

Chen¹,

Peng²

2016

J Plant Pathol Microbiol

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Penicillium digitatum causes green mold decay on citrus fruit, resulting in severe economic losses to citrus growers and packers worldwide. The present study is to evaluate the control of citrus green mold by volatiles produced by Enterobacter cloacae. An E. cloacae strain isolated from plant rhizospheres was able to produce three volatile organic compounds, which were identified as butyl acetate, phenylethyl alcohol, and 4,5-dimethyl-1-hexene by GC/MS chromatography. The volatile compounds produced by E. cloacae inhibited conidial germination and hyphal elongation of P. digitatum and reduced green mold severity. E. cloacae cultured at temperatures ranging from 16°C to 28°C, at pH values ≤6, or in a substrate carrier (sphagnum moss, vermiculite, or perlite) provided superior control against P. digitatum. A laboratory formulation using E. cloacae and perlite protected citrus fruit from green mold up to 22 days and its effectiveness outperformed fungicide application at room temperature (~25°C). The results implicate practical application of E. cloacae as a biofumigant for controlling citrus postharvest decay caused by P. digitatum. Significantly, the study provides a model for future research on how to formulate an effective biocontrol agent for disease management.

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Production of volatile organic compounds by Aureobasidium pullulans as a potential mechanism of action against postharvest fruit pathogens

Cited by 197 publications

References 35 publications

Combining biocontrol agents with different mechanisms of action in a strategy to control Botrytis cinerea on grapevine

Combining biocontrol agents with different mechanisms of action in a strategy to control Botrytis cinerea on grapevine

Aureobasidium pullulans volatilome identified by a novel, quantitative approach employing SPME-GC-MS, suppressed Botrytis cinerea and Alternaria alternata in vitro

Inhibition of Penicillium digitatum and Citrus Green Mold by Volatile Compounds Produced by Enterobacter cloacae

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