Production of uniform emulsion droplets using a simple fluidic device with a peristaltic pump

Hwang, Seok Joon; Moon, Seung Kwan; Kim, Sung Eun; Kim, Jung Hyun; Choi, Sung Wook

doi:10.1007/s13233-014-2074-1

Cited by 7 publications

(7 citation statements)

References 19 publications

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“…The microsphere sizes produced at a particular stir rate appear to form a skewed distribution as also noted by Zhang et al 41 Other groups similarly reported that the average microsphere diameter decreased with the increasing stirring rate. 42 (PLGA)-based emulsion began to separate out into multiple phases soon after formation and this phase separation was exploited to produce microspheres with variable pore sizes. 22 Using either CSTR or T-microfluidics to form particles did not significantly alter the internal pore size of the resulting microsphere, as the pore size is determined at the HIPE formation stage.…”

Section: Discussionmentioning

confidence: 99%

Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis

et al. 2018

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Porous microspheres have the potential for use as injectable bone fillers to obviate the need for open surgery. Successful bone fillers must be able to support vascularisation since tissue engineering scaffolds often cease functioning soon after implantation due to a failure to vascularise rapidly. Here, we test the angiogenic potential of a tissue engineered bone filler based on a photocurable acrylate-based high internal phase emulsion (HIPE). Highly porous microspheres were fabricated via two processes, which were compared. One was taken forward and investigated for its ability to support human mesenchymal progenitor cells and angiogenesis in a chorioallantoic membrane (CAM) assay. Porous microspheres with either a narrow or broad size distribution were prepared via a T-junction microfluidic device or by a controlled stirred-tank reactor of the HIPE water in oil in water (w/o/w), respectively. Culture of human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells showed proliferation over 11 days and formation of cell-microsphere aggregates. In-vitro , hES-MP cells were found to migrate into microspheres through their surface pores over time. The presence of osteoblasts, differentiated from the hES-MP cells, was evidenced through the presence of collagen and calcium after 30 days. Microspheres pre-cultured with cells were implanted into CAM for 7 days and compared with control microspheres without pre-cultured cells. The hES-MP seeded microspheres supported greater angiogenesis, as measured by the number of blood vessels and bifurcations, while the empty scaffolds attracted host chick cell ingrowth. This investigation shows that controlled fabrication of porous microspheres has the potential to create an angiogenic, bone filling material for use as a cell delivery vehicle.

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Section: Discussionmentioning

confidence: 99%

Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis

et al. 2018

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“…The water sample undergoes circulated flow in the fluidic system to prevent liquid splashing. The liquid fluidic system consists of a peristaltic pump, experimental stand, glass tube, and beaker [ 29 ]. The peristaltic pump is used to ensure the circulating flow of the liquid and regulate the jet velocity of the liquid.…”

Section: Methodsmentioning

confidence: 99%

Estimation of the Fe and Cu Contents of the Surface Water in the Ebinur Lake Basin Based on LIBS and a Machine Learning Algorithm

Zhang

Kung

et al. 2018

IJERPH

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Traditional technology for detecting heavy metals in water is time consuming and difficult and thus is not suitable for quantitative detection of large samples. Laser-induced breakdown spectroscopy (LIBS) can identify multi-state (such as solid, liquid, and gas) substances simultaneously, rapidly and remotely. In this study, water samples were collected from the Ebinur Lake Basin. The water samples were subjected to LIBS to extract the characteristic peaks of iron (Fe) and copper (Cu). Most of the quantitative analysis of LIBS rarely models and estimates the heavy metal contents in natural environments and cannot quickly determine the heavy metals in field water samples. This study creatively uses the Fe and Cu contents in water samples and the characteristics of their spectral curves in LIBS for regression modelling analysis and estimates their contents in an unknown water body by using LIBS technology and a machine learning algorithm, thus improving the detection rate. The results are as follows: (1) The Cu content of the Ebinur Lake Basin is generally higher than the Fe content, the highest Fe and Cu contents found within the basin are in the Ebinur Lake watershed, and the lowest are in the Jing River. (2) A number of peaks from each sample were found of the LIBS curve. The characteristic analysis lines of Fe and Cu were finally determined according to the intensities of the Fe and Cu characteristic lines, transition probabilities and high signal-to-background ratio (S/B). Their wavelengths were 396.3 and 324.7 nm, respectively. (3) The relative percent deviation (RPD) of the Fe content back-propagation (BP) network estimation model is 0.23, and the prediction ability is poor, so it is impossible to accurately predict the Fe content of samples. In the estimation model of BP network of Cu, the coefficient of determination (R2) is 0.8, the root mean squared error (RMSE) is 0.1, and the RPD is 1.79. This result indicates that the BP estimation model of Cu content has good accuracy and strong predictive ability and can accurately predict the Cu content in a sample. In summary, estimation based on LIBS improved the accuracy and efficiency of Fe and Cu content detection in water and provided new ideas and methods for the accurate estimation of Fe and Cu contents in water.

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“…When we compare the monodispersity values given in the literature, which mainly used displacement (syringe) or pressure pumps, we see a victory of syringe pump-driven systems that are immune to the pressure fluctuations and are primarily affected by the variation of inlet volumetric flow rates (Bauer et al, 2010;Hwang et al, 2014;Link et al, 2004;Wang et al, 2015;Xu and Nakajima, 2004). The intrinsic internal fluctuation's effect on droplet monodispersity in syringe pump-driven systems is less than pressuredriven systems (Kalantarifard et al, 2018).…”

mentioning

confidence: 94%

Theoretical and experimental limits of monodisperse droplet generation

Kalantarifard

Alizadeh-Haghighi

Saateh

et al. 2021

Chemical Engineering Science

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Production of uniform emulsion droplets using a simple fluidic device with a peristaltic pump

Cited by 7 publications

References 19 publications

Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis

Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis

Estimation of the Fe and Cu Contents of the Surface Water in the Ebinur Lake Basin Based on LIBS and a Machine Learning Algorithm

Theoretical and experimental limits of monodisperse droplet generation

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