Production of Tyrosinase Inhibitor from Saccharomyces cerevisiae

Abstract

: Physiological functionalities of culture concentrates from various fungi were investigated. The culture concentrates from Saccharomyces cerevisiae Y277-3 showed the highest tyrosinase inhibitory activity of 42.7%. Among mold physiological functionalities, the culture concentrates from Aspergillus orygae CN20-3-1-4 showed the highest antioxidant activity of 15.8%. The other functionalities of fungi were very low or not detected. The intracellular tyrosinase inhibitor from Saccharomyces cerevisiae Y277-3, which showed the highest physiological functionalities was maximally produced when the strain was cultured in PD broth at 30 o C for 24 h.

Exploring the Health Benefits of Yeast Isolated from Traditional Fermented Foods in Korea: Anti-Inflammatory and Functional Properties of Saccharomyces and Non-Saccharomyces Strains

“…As a result of confirming the whitening effect by measuring the extracellular tyrosinase inhibition activity of the isolates, five isolates demonstrated <10% activity ( Table 2 ). Reportedly, the extracellular activity of wild-type yeast was 10–20%, indicating a similar trend to the five isolates in this study [ 46 ]. In contrast, the intracellular activity was approximately 20% higher than the extracellular activity [ 1 , 46 ].…”

“…Reportedly, the extracellular activity of wild-type yeast was 10–20%, indicating a similar trend to the five isolates in this study [ 46 ]. In contrast, the intracellular activity was approximately 20% higher than the extracellular activity [ 1 , 46 ]. Therefore, wild yeast possesses tyrosinase inhibitory activity, but does not secrete it extracellularly; further research is needed.…”

Isolation and Identification of Wild Yeasts from Soils of Fields in Daejeon Metropolitan City and Chungcheongnam-do, Korea

“…최근 진핵 생물의 유전자 발현을 위한 분자생물학적 연 구에서 host로서 매우 중요하게 이용되고 있을 뿐만 아니 라 혈관신생억제물질 [2], 항고혈압성 안지오텐신 전환효소 저해물질 [3,4], 혈전용해 활성물질 [5], 항치매성 β-secretase 저해물질 [6]과 acetylcholinesterase 저해물질 [7], 미백 성 tyrosinase 저해물질 [8], 효모 핵산물질의 생리기능성 [9] 핵산물질 등 고부가가치의 건강 소재 생산 균주로서 효모에 관한 연구가 진행되고 있다. 한편, 지금까지 효모는 주로 발효식품 등에서 분리되어 Saccharomyces cerevisiae 등의 일부만이 발효식품산업에 응 용되고 있다.…”

Physiological Functionality of Fermented Pear Fruitlet Product Made by Mixed Fermentation of Saccharomyces cerevisiae, Kluyveromyces fragilis and Lactobacillus plantarum

“…Tyrosinase 저해활성은 시료 500 µL에 5 mM L-DOPA 0.2 mL, 0.1 M sodium phosphate 완충용액(pH 6.0)를 혼 합한 후 tyrosinase 11 U을 첨가하여 35 o C에서 2분간 반응 시킨 다음 475 nm에서 흡광도를 측정하여 시료액 무첨가 대조구의 값과 비교하여 활성을 계산하였다 (Jang et al, 2012a). Jang et al, 2012b).…”