Production of two types of ice crystal-controlling proteins in Antarctic bacterium

Kawahara, Hidehisa; Nakano, Yoko; Omiya, Kazuhiro; Muryoi, Naomi; Nishikawa, Jiro; Obata, Hitoshi

doi:10.1016/s1389-1723(04)00271-3

Cited by 35 publications

(30 citation statements)

References 17 publications

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“…Of these bacteria, only the Pseudomonas species could swim at close to 0°C. These include P. borealis [31,32] and P. syringae (INP+) [33], both of which contain ice-nucleating protein (INP) on their outer membranes, P. syringae cit7del mutant (INP−), which lacks ice-nucleating activity [34] , and P. fluorescens , which was suggested to express both INP and AFP [35]. Pseudomonas borealis freely swim in the solution but do not bind the ice or sense it in any visible way (figure 4 c ; electronic supplementary material, movie S3).…”

Section: Resultsmentioning

confidence: 99%

“…These findings emphasized that Mp IBP might have a different role in Nature from the DUF3494 bacterial IBPs, which seem to control the structure of ice surrounding the microorganism [40]. Other ice-active proteins were reported in bacteria from harsh environments where the temperature drops way below zero during winter such as Antarctic and cold temperate soils [35,41] or high Arctic plant rhizosphere [9]. At these temperatures, it is likely that freezing will be tolerated and that IBPs could serve to inhibit IR.…”

Section: Discussionmentioning

confidence: 99%

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Putting life on ice: bacteria that bind to frozen water

Dolev

Bernheim

Guo

et al. 2016

J. R. Soc. Interface.

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Ice-binding proteins (IBPs) are typically small, soluble proteins produced by cold-adapted organisms to help them avoid ice damage by either resisting or tolerating freezing. By contrast, the IBP of the Antarctic bacterium Marinomonas primoryensis is an extremely long, 1.5 MDa protein consisting of five different regions. The fourth region, a 34 kDa domain, is the only part that confers ice binding. Bioinformatic studies suggest that this IBP serves as an adhesin that attaches the bacteria to ice to keep it near the top of the water column, where oxygen and nutrients are available. Using temperature-controlled cells and a microfluidic apparatus, we show that M. primoryensis adheres to ice and is only released when melting occurs. Binding is dependent on the mobility of the bacterium and the functionality of the IBP domain. A polyclonal antibody raised against the IBP region blocks bacterial ice adhesion. This concept may be the basis for blocking biofilm formation in other bacteria, including pathogens. Currently, this IBP is the only known example of an adhesin that has evolved to bind ice.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Putting life on ice: bacteria that bind to frozen water

Dolev

Bernheim

Guo

et al. 2016

J. R. Soc. Interface.

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“…Some bacteria such as Pseudomonas fluorescens KUAF-68 and Pseudomonas borealis DL7 have been reported to have both antifreeze and ice nucleation activity (Kawahara et al, 2004;Wilson et al, 2006). Having these two properties has been suggested to enhance the freezetolerance survival of bacteria by maintaining small ice crystals with ice recrystallization inhibition protecting against freeze-thaw stress with antifreeze proteins (Xu et al, 1998), minimizing damage from explosive ice crystal growth and stabilizing the outer membrane with the low thermal hysteresis value (Xu et al, 1998), and minimizing the supercooling point with ice nucleation proteins (Kawahara et al, 2004). In our study, under our experimental conditions, the highest percentage was observed in urban snow samples (0.1 %), and the lowest was observed in blowing snow (0.03 %).…”

Section: R Mortazavi Et Al: Arctic Microbial and Next-generation Sementioning

confidence: 99%

Arctic microbial and next-generation sequencing approach for bacteria in snow and frost flowers: selected identification, abundance and freezing nucleation

2015

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Abstract. During the spring of 2009, as part of the OceanAtmosphere-Sea Ice-Snowpack (OASIS) campaign in Barrow, Alaska, USA, we examined the identity, population diversity, freezing nucleation ability of the microbial communities of five different snow types and frost flowers. In addition to the culturing and gene-sequence-based identification approach, we utilized a state-of-the-art genomic next-generation sequencing (NGS) technique to examine the diversity of bacterial communities in Arctic samples. Known phyla or candidate divisions were detected (11-18) with the majority of sequences (12.3-83.1 %) belonging to one of the five major phyla: Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes, and Cyanobacteria. The number of genera detected ranged from, 101-245. The highest number of cultivable bacteria was observed in frost flowers (FFs) and accumulated snow (AS) with 325 ± 35 and 314 ± 142 CFU m L −1 , respectively; and for cultivable fungi 5 ± 1 CFU m L −1 in windpack (WP) and blowing snow (BS). Morphology/elemental composition and ice-nucleating abilities of the identified taxa were obtained using high resolution electron microscopy with energy-dispersive X-ray spectroscopy and ice nucleation cold-plate, respectively. Freezing point temperatures for bacterial isolates ranged from −20.3 ± 1.5 to −15.7 ± 5.6 • C, and for melted snow samples from −9.5 ± 1.0 to −18.4 ± 0.1 • C. An isolate belonging to the genus Bacillus (96 % similarity) had ice nucleation activity of −6.8 ± 0.2 • C. Comparison with Montreal urban snow, revealed that a seemingly diverse community of bacteria exists in the Arctic with some taxa possibly originating from distinct ecological environments. We discuss the potential impact of snow microorganisms in the freezing and melting process of the snowpack in the Arctic.

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“…In fact, some researchers argue that both proteins use the same mechanism, differing in function due to molecular size and concentration [9–13]. Not surprisingly, some bacteria have been found with both antifreeze and ice nucleation activity [4, 7, 14, 15]. Therefore, Kawahara [1] has proposed the term ice crystal controlling protein (ICC) to encompass both protein classes with an affinity for ice.…”

Section: Introductionmentioning

confidence: 99%

“…Within the last two decades, antifreeze proteins have been found in variety of bacteria from cold habitats [4, 6, 14, 18, 34, 35]. However, for the majority of these instances, only basic characterization of the proteins has been documented.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Ice Crystal Controlling Proteins

2014

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Across the world, many ice active bacteria utilize ice crystal controlling proteins for aid in freezing tolerance at subzero temperatures. Ice crystal controlling proteins include both antifreeze and ice nucleation proteins. Antifreeze proteins minimize freezing damage by inhibiting growth of large ice crystals, while ice nucleation proteins induce formation of embryonic ice crystals. Although both protein classes have differing functions, these proteins use the same ice binding mechanisms. Rather than direct binding, it is probable that these protein classes create an ice surface prior to ice crystal surface adsorption. Function is differentiated by molecular size of the protein. This paper reviews the similar and different aspects of bacterial antifreeze and ice nucleation proteins, the role of these proteins in freezing tolerance, prevalence of these proteins in psychrophiles, and current mechanisms of protein-ice interactions.

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Production of two types of ice crystal-controlling proteins in Antarctic bacterium

Cited by 35 publications

References 17 publications

Putting life on ice: bacteria that bind to frozen water

Putting life on ice: bacteria that bind to frozen water

Arctic microbial and next-generation sequencing approach for bacteria in snow and frost flowers: selected identification, abundance and freezing nucleation

Bacterial Ice Crystal Controlling Proteins

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