Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.)

Dehghan, Esmail; Häkkinen, Suvi T.; Oksman-Caldentey, Kirsi-Marja; Ahmadi, Farajollah Shahriari

doi:10.1007/s11240-012-0127-8

Cited by 87 publications

(49 citation statements)

References 35 publications

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“…Our analysis indicates that chromosome doubling has a significant influence on secondary metabolite concentrations. Similar results have been reported for other plant species (Banyai et al, 2010;Majdi et al, 2010;Mishra et al, 2010;Caruso et al, 2011;Dehghan et al, 2012;Abdoli et al, 2013;Lavania, 2013;Dixit and Chaudhary, 2014). Phenolic compounds are active in pathogen resistance, pigment production, and protection against ultraviolet radiation (Winkel-Shirley, 2001); they also qualitatively and quantitatively improve the biochemical profile of plants (Maruška et al, 2010).…”

Section: Phenolic Compounds In Triploid and Hexaploid M × Giganteussupporting

confidence: 80%

“…Phenolic compounds are active in pathogen resistance, pigment production, and protection against ultraviolet radiation (Winkel-Shirley, 2001); they also qualitatively and quantitatively improve the biochemical profile of plants (Maruška et al, 2010). Polyploidy often enhances the expression and concentration of some secondary metabolites and defence chemicals (DeJesus-Gonzalez and Weathers, 2003;Jones et al, 2008;Nilanthi et al, 2009;Dhooghe et al, 2011;Dehghan et al, 2012;Hsia et al, 2013;Gomes et al, 2014;Zahedi et al, 2014).…”

Section: Phenolic Compounds In Triploid and Hexaploid M × Giganteusmentioning

confidence: 99%

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Assessment of morphological and phytochemical attributes in triploid and hexaploid plants of the bioenergy crop Miscanthus×giganteus

Ghimire

Seong

Nguyen

et al. 2016

Industrial Crops and Products

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Section: Phenolic Compounds In Triploid and Hexaploid M × Giganteussupporting

confidence: 80%

Section: Phenolic Compounds In Triploid and Hexaploid M × Giganteusmentioning

confidence: 99%

Assessment of morphological and phytochemical attributes in triploid and hexaploid plants of the bioenergy crop Miscanthus×giganteus

Ghimire

Seong

Nguyen

et al. 2016

Industrial Crops and Products

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“…Colchicine-induced autotetraploid plants has been reported in several medicinal plants including Tanacetum parthenium L. (Saharkhiz 2007), Artemisia annua L. (Banyai et al 2010), Dioscorea zingiberensis (Zhang et al 2010), Dracocephalum moldavica L. (Omidbaigi et al 2010b) and Hyoscyamus muticus (Dehghan et al 2012). …”

Section: Introductionmentioning

confidence: 99%

Overproduction of valuable methoxylated flavones in induced tetraploid plants of Dracocephalum kotschyi Boiss

et al. 2014

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BackgroundPloidy manipulation is considered an efficient method to increase production potential of medicinally important compounds. Dracocephalum kotschyi Boiss. is an endangered medicinal plant of Iran. Various concentrations of colchicine (0.05, 0.10, 0.20, and 0.50% w/v) were applied to shoot apical meristems of D. kotschyi seedlings in two and four-leaf stages to induce tetraploidy.ResultsAccording to the results, 0.5% (w/v) of colchicine can be effective for polyploidy induction in D. kotschyi. Putative tetraploids were selected by morphological and microscopic characteristics and their ploidy level was confirmed by flow cytometry analysis and chromosome counting. The chromosome number of original diploid plant was confirmed to be 2n = 2× = 20 whereas that of the tetraploid plant was 2n = 4× = 40. Tetraploid and mixoploid plants showed different morphological, physiological and microscopic characteristics from those of diploid counterparts. The total content of flavonoids was increased from 1583.28 in diploids to 1890.07 (μg/g DW) in stable tetraploids.ConclusionHigh-Performance Liquid Chromatography with Diode-Array Detection (HPLC–DAD) confirmed over accumulation of methoxylated hydroxyflavones in solid tetraploid plants of D. kotschyi.Electronic supplementary materialThe online version of this article (doi:10.1186/1999-3110-55-22) contains supplementary material, which is available to authorized users.

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“…Instability of nuclear DNA content, which is often observed in terms of induced polyploidy or aneuploidy, is frequently observed in plant cell suspensions (Baebler et al 2005; Creemers-Molenaar et al 1992). Variable ploidy levels have also been shown to affect secondary metabolite production in seedlings of Hypericum perforatum (Kosuth et al 2003),hairy root cultures of Artemisia annua (De Jesus-Gonzalez and Weathers 2003) and Hyoscyamus muticus (Dehghan et al 2012). Studies on genomic stability of Taxus cell cultures immediately following culture initiation (two-year timeframe) have been reported (Baebler et al 2005) and indicate heterogeneity in genomic stability amongst cell lines; however, paclitaxel levels have not been correlated to nuclear DNA content or ploidy levels in well-established Taxus suspension cultures.…”

Section: Introductionmentioning

confidence: 99%

Cellular aggregation is a key parameter associated with long term variability in paclitaxel accumulation in Taxus suspension cultures

Patil

Kolewe

Roberts

2012

Plant Cell Tiss Organ Cult

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Plant cell cultures provide a renewable source for synthesis and supply of commercially valuable plant-derived products, particularly for secondary metabolites. However, instability in product yields over multiple passages has hampered the efficient and sustainable use of this technology. Paclitaxel accumulation in Taxus cell suspension culture was quantified over multiple passages and correlated to mean aggregate size, extracellular sugar level, ploidy, and cell cycle distribution. Paclitaxel levels varied approximately 6.9-fold over the six-month timeframe investigated. Of all of the parameters examined, only mean aggregate size correlated with paclitaxel accumulation, where a significant negative correlation (r = − 0.75, p < 0.01) was observed. These results demonstrate the relevance of measuring, and potentially controlling, aggregate size during long term culture passages, particularly for plant suspensions where industrially relevant secondary metabolites are not pigmented to enable rapid culture selection.

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Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.)

Cited by 87 publications

References 35 publications

Assessment of morphological and phytochemical attributes in triploid and hexaploid plants of the bioenergy crop Miscanthus×giganteus

Assessment of morphological and phytochemical attributes in triploid and hexaploid plants of the bioenergy crop Miscanthus×giganteus

Overproduction of valuable methoxylated flavones in induced tetraploid plants of Dracocephalum kotschyi Boiss

Cellular aggregation is a key parameter associated with long term variability in paclitaxel accumulation in Taxus suspension cultures

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