Production of transgenic soybean lines expressing the bean pod mottle virus coat protein precursor gene

Abstract

Soybean (Glycine max. Merrill. cv. Fayette) cotyledonary nodes were transformed with bean pod mottle virus (BPMV) coat protein precursor (CP-P) gene via Agrobacterium-mediated transformation. The transformation rate was low, and only five primary transformants derived from five different cotyledons were obtained from 400 original cotyledons. Southern blot hybridization verified the integration of the BPMV CP-P gene. Inheritance and expression of this gene in R1 plants were also demonstrated. About 30% of R2 plants derived from one transgenic line showed complete resistance to BPMV infection, as assessed by symptomatology and ELISA, suggesting that homozygous, but not hemizygous, plants exhibit the resistant phenotype.

Two large-insert soybean genomic libraries constructed in a binary vector: applications in chromosome walking and genome wide physical mapping

“…However, there are only a few reports of transformation of large DNA fragments (>70 kb) into a plant genome (Hamilton et al 1996;Liu et al 1999). Although soybean cells can be successfully transformed using a number of different methods (Rong et al 1996), it has been recalcitrant to transformation due to a low efficiency in the regeneration of transformed cells into fertile plants (Singh et al 1998). Therefore, functional genomics and xenogenetics can provide more targets for soybean transformation than can be analyzed without large-insert transformation methods.…”

Advancements in Transgenic Soy: From Field to Bedside

“…This is the same approach that has been used by several groups to develop transgenic viral resistance in soybean. One of the first groups to investigate this approach was Di et al, who produced a soybean that was resistant to bean pod mottle virus (BPMV) [23]. This was done by introducing a BPMV coat protein into the soybean genome.…”

Detection, Understanding and Controlof Soybean Mosaic Virus

“…It has been used to develop genetic resistance against a wide range of plant viruses including potyviruses (Powell-Abel et al, 1986;Lius et al, 1995;Di et al, 1996;Wang et al, 2009). This approach requires generation of transgenic plants with partial viral genomes.…”