Production of Transgenic Ruminants by DNA Microinjection

Niemann, Heinrich; Döpke, Hans-Hermann; Hadeler, Klaus‐Gerd

doi:10.1016/b978-0-08-057480-6.50017-4

Cited by 9 publications

(9 citation statements)

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“…Micro injection of DNA directly into zygotic pronuclei has been used for several years in livestock animals [ 11,12 ] , but zygotes (1-cell embryos) are diffi cult to obtain and manipulate (those of species such as S. scrofa are opaque, due to high lipid content), and the effi ciency of integration and transgenerational transmission of the foreign DNA remains low [ 13 ] . Relatively recently, this problem has been addressed by somatic cell nuclear transfer (NT) (Fig.…”

Section: Using Micromanipulation To Engineer Livestock Genomesmentioning

confidence: 99%

Livestock Production via Micromanipulation

Ōnishi

Perry

2012

Practical Manual of in Vitro Fertilization

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The use of micromanipulation techniques in the production of livestock mammals, focusing on intracytoplasmic sperm injection (ICSI) and the pig, Sus scrofa , is discussed in this chapter. ICSI is a powerful method for assisted fertilization. It is typically employed in cases in which semen characteristics are insuffi cient for conventional in vitro fertilization (IVF). Unlike IVF, ICSI mechanically delivers the sperm deep inside the egg cytoplasm by injection through a micropipette. We summarize the use of micromanipulation techniques in the production of livestock mammals, focusing on intracytoplasmic sperm injection (ICSI) and the pig, Sus scrofa . ICSI is a powerful method for assisted fertilization. It is typically employed where semen characteristics are insuffi cient for conventional in vitro fertilization (IVF), in which sperm and eggs are mixed and fuse in culture. Unlike IVF, ICSI mechanically delivers the sperm deep inside the egg cytoplasm by injection through a micropipette.Mammalian ICSI was fi rst demonstrated in hamster oocytes [ 1 ] and subsequently applied to humans to overcome impaired fertility [ 2 ] . In livestock, ICSI is also used as a procedure for fertilization, but its purpose is not restricted to impaired male fertility. Although circumstances do not ordinarily justify ICSI for breeding normal livestock, there are clear exceptions. Where sperm-containing ejaculates cannot be obtained, a small number of sperm may be obtained by biopsy, with ICSI as the method of choice for delivery into oocyte. Owing to its technical robustness, ICSI is especially benefi cial where the breeding male stock has high genetic merit. These applications are now considered in greater detail.

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Section: Using Micromanipulation To Engineer Livestock Genomesmentioning

confidence: 99%

Livestock Production via Micromanipulation

Ōnishi

Perry

2012

Practical Manual of in Vitro Fertilization

View full text Add to dashboard Cite

show abstract

“…Von wissenschaftlicher und auch von kommerzieller Seite her interessant ist das so genannte Gene Pharming (Krimpenfort et al 1991, Niemann et al 2002. Hier versucht man vor allem Proteine, die eine komplizierte posttranskriptionelle Modifikation (z.…”

Section: Produktiver Nutzenunclassified

“…B. die Expression bestimmter Proteine in der Milch der Tiere und somit der Nutzen als Bioreaktor (Maga u. Murray 1995, Rudolph 1999: die Laktationsphase wird nach ein bis zwei Jahren erreicht, verbesserte Wollproduktion (Damak et al 1996a(Damak et al , 1996b, Verbesserungen des Verdauungstraktes der Wiederkäuer (Ward 2000), Wachstum und Entwicklung (Murray et al 1989) oder Krankheitsresistenz (Clements et al 1994, Niemann et al 2002. Es gab auch Ansätze zur Xenotransplantation (Colman 1996, Espanion und Niemann 1996, Amoah u. Gelaye 1997.…”

Section: Transgene Schafe Und Ziegenunclassified

“…Biomedizinisch wichtige Proteine wurden in verschiedenen transgenen Tieren synthetisiert und durch die Milch sekretiert, z. B. in Mäusen, Kaninchen, Schafen und Ziegen (Niemann et al 2002). Dabei wurden zum Teil hohe Expressionsraten gefunden, beispielsweise 7 bis 25 mg/ml α-1-Antitrypsin in der Maus und im Schaf (Archibald et al 1990) oder 3 mg/ml Plasminogenaktivator in Ziegen (Ebert et al 1991).…”

Section: Transgene Rinderunclassified

“…Sie können operativ unter Ultraschalldiagnostik gewonnen bzw. aspiriert werden (Bungartz et al 1995, Niemann et al 2002. Grundsätzlich lassen sich Kühe auch mit PMSG oder FSH superovulieren.…”

Section: Transgene Rinderunclassified

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