Production of transgenic fish: introduction and expression of chicken δ-crystallin gene in medaka embryos

Ozato, Kenjiro; Kondoh, Hisato; Inohara, Hiroyuki; Isobe, Takashi; Wakamatsu, Yuko; Okada, T.

doi:10.1016/0045-6039(86)90100-4

Cited by 158 publications

(52 citation statements)

References 19 publications

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“…Transferring the foreign genes into the germinal vesicle (Ozato et al, 1986;Tsai et al, 1995b) or sperm (Tsai et al, 1995a;1997) before fertilization still resulted in mosaic and variegated patterns of transgene expression. Use of a homologous promoter also did little to reduce mosaicism (Fu et al, 2000;Higashijima et al, 1997;Ju et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Enhanced expression and stable transmission of transgenes flanked by inverted terminal repeats from adeno‐associated virus in zebrafish

Hsiao

Hsieh

Tsai

2001

Developmental Dynamics

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Section: Introductionmentioning

confidence: 99%

Enhanced expression and stable transmission of transgenes flanked by inverted terminal repeats from adeno‐associated virus in zebrafish

Hsiao

Hsieh

Tsai

2001

Developmental Dynamics

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“…This and earlier attempts to use the medaka for production of stable transgenic fish (Ozato et al 1986(Ozato et al , 1989 suggested that this organism might be a suitable experimental system for gene transfer experiments, because it was shown that the medaka embryo is easier to micromanipulate and to inject, and tolerates a wider concentration range of injected foreign DNA than other species commonly used for fish developmental biology, e.g. the zebrafish (Stuart et al 1988;Vielkind et al 1990).…”

Section: Introductionmentioning

confidence: 99%

Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes)

Winkler

Vielkind²,

Schartl

1991

Mol Gen Genet

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Summary. Species of small fish are becoming useful tools for studies on vertebrate development. Wehave investigated the developing embryo of the Japanese medaka for its application as a transient expression system for the in vivo analysis of gene regulation and function. The temporaland spatial expression patterns ofbacterial chloramphenicol acetyltransferase and galactosidase reporter genes injected in supercoiled plasmid form into the cytoplasm of one cell of the two-cell stage embryo was promoter-specific. The transient expression was found to be mosaic within the tissue and organs reflecting the unequal distribution of extrachromosomal foreign DNA and the intensive cell mixing movements that occur in fish embryogenesis. The expression data are consistent with data on DNA fate. Foreign DNA persisted during embryogenesis and was still detectable in some 3-and 9-month-old adult fish; it was found in high molecular weight form as weil as in circular plasmid conformations. The DNA was replicated during early and late embryogenesis. Our data indicate that the developing medaka embryo is a powerful in vivo assay system for studies of gene regulation and function.

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“…Since the generation of transgenic goldfish through growth hormone gene transfer has been succeeded (Zhu et al 1985), many of other transgenic fish are successfully constructed, including rainbow trout (Chourrout et al 1986), medaka (Ozato et al 1986), tilapia (Martinez et al 1996), salmon (Fletcher et al 1988), zebrafish (Stuart et al 1988), channel catfish (Dunham et al 1987), and common carp (Zhang et al 1990). Meanwhile, a number of gene transfer approaches including direct microinjection (Zhong et al 2002), retrovirus infection (Linney et al 1999), electroporation (Xie et al 1993), sperm-mediation (Khoo et al 1992) and particle-gun bombardment (Yamauchi et al 2000) were successfully used to deliver foreign DNA into different fish species, but direct microinjection of DNA into the fertilized eggs at one-cell stage has been the prevalent method.…”

Section: Introductionmentioning

confidence: 99%

Gene transfer and mutagenesis mediated by Sleeping Beauty transposon in Nile tilapia (Oreochromis niloticus)

Long

et al. 2013

Transgenic Res

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The success of gene transfer has been demonstrated in many of vertebrate species, whereas the efficiency of producing transgenic animals remains pretty low due to the random integration of foreign genes into a recipient genome. The Sleeping Beauty (SB) transposon is able to improve the efficiency of gene transfer in zebrafish and mouse, but its activity in tilapia (Oreochromis niloticus) has yet to be characterized. Herein, we demonstrate the potential of using the SB transposon system as an effective tool for gene transfer and insertional mutagenesis in tilapia. A transgenic construct pT2/tiHsp70-SB11 was generated by subcloning the promoter of tilapia heat shock protein 70 (tiHsp70) gene, the SB11 transposase gene and the carp b-actin gene polyadenylation signal into the second generation of SB transposon. Transgenic tilapia was produced by microinjection of this construct with in vitro synthesized capped SB11 mRNA. SB11 transposon was detected in 28.89 % of founders, 12.9 % of F1 and 43.75 % of F2. Analysis of genomic sequences flanking integrated transposons indicates that this transgenic tilapia line carries two copies of SB transposon, which landed into two different endogenous genes. Induced expression of SB11 gene after heat shock was detected using reverse transcription PCR in F2 transgenic individuals. In addition, the Cre/loxP system was introduced to delete the SB11 cassette for stabilization of gene interruption and biosafety. These findings suggest that the SB transposon system is active and can be used for efficient gene transfer and insertional mutagenesis in tilapia.

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Production of transgenic fish: introduction and expression of chicken δ-crystallin gene in medaka embryos

Cited by 158 publications

References 19 publications

Enhanced expression and stable transmission of transgenes flanked by inverted terminal repeats from adeno‐associated virus in zebrafish

Enhanced expression and stable transmission of transgenes flanked by inverted terminal repeats from adeno‐associated virus in zebrafish

Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes)

Gene transfer and mutagenesis mediated by Sleeping Beauty transposon in Nile tilapia (Oreochromis niloticus)

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