Production of transgenic beef cattle rich in n-3 PUFAs by somatic cell nuclear transfer

Chen, Gong; Fu, Changzhen; Wang, Hong; Adoligbé, Camus; Wei, Song; Li, Shijun; Jiang, Bijie; Wang, Hongbao; Liu, Zan

doi:10.1007/s10529-015-1827-z

Cited by 15 publications

(11 citation statements)

References 16 publications

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“…The introduction of the Fat-1 gene in mouse, pig, sheep, cattle, and cow resulted in high levels of n-3 PUFAs and significantly lower n-6/n-3 PUFA ratios in their tissues [10][11][12]23,46,47]. In our study, the same results were obtained by comparing n-6 and n-3 PUFA contents between 100-day transgenic fetuses and wild type fetuses.…”

Section: Discussionsupporting

confidence: 81%

Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep

Luo

Zheng

Yang

et al. 2020

IJMS

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Transgenic technology has huge application potential in agriculture and medical fields, such as producing new livestock varieties with new valuable features and xenotransplantation. However, how an exogenous gene affects the host animal’s gene regulation networks and their health status is still poorly understood. In the current study, Fat-1 transgenic sheep were generated, and the tissues from 100-day abnormal (DAF_1) and normal (DAF_2) fetuses, postnatal lambs (DAF_4), transgenic-silencing (DAFG5), and -expressing (DAFG6) skin cells were collected and subjected to transcriptome sequencing, and their gene expression profiles were compared in multiple dimensions. The results were as follows. For DAF_1, its abnormal development was caused by pathogen invasion but not the introduction of the Fat-1 gene. Fat-1 expression down-regulated the genes related to the cell cycle; the NF-κB signaling pathway and the PI3K/Akt signaling pathway were down-regulated, and the PUFAs (polyunsaturated fatty acids) biosynthesis pathway was shifted toward the biosynthesis of high-level n-3 LC-PUFAs (long-chain PUFAs). Four key node genes, FADS2, PPARA, PRKACA, and ACACA, were found to be responsible for the gene expression profile shift from the Fat-1 transgenic 100-day fetus to postnatal lamb, and FADS2 may play a key role in the accumulation of n-3 LC-PUFAs in Fat-1 transgenic sheep muscle. Our study provides new insights into the FUFAs synthesis regulation in Fat-1 transgenic animals.

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Section: Discussionsupporting

confidence: 81%

Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep

Luo

Zheng

Yang

et al. 2020

IJMS

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“…For example, feeding beef cattle with rumen‐protected fish oil supplements or oil seeds can be used to increase C20:5 n ‐3 and C22:6 n ‐3 concentrations. Also, there have been efforts to modulate the genetics of bovines to increase total n ‐3 FA: Cheng et al . demonstrated that the codon‐optimized C. elegans mfat1 gene can be functionally expressed in beef cattle and converts n ‐6 PUFAs to n ‐3 PUFAs.…”

Section: Ruminant Meat and Human Healthmentioning

confidence: 99%

Impacts of fat from ruminants' meat on cardiovascular health and possible strategies to alter its lipid composition

Vargas‐Bello‐Pérez

Larraín

2017

J Sci Food Agric

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In the last few decades there has been increased consumer interest in the fatty acid (FA) composition of ruminant meat due to its content of saturated FAs, which have been implicated in diseases associated with modern life. However, recent studies have questioned the recommendations to reduce intake of fat, saturated FAs and cholesterol as a means of reducing the risk of cardiovascular disease. Interestingly, ruminant meat has some bioactive lipids such as C18:1t11 and C18:2 c9, t11 which have been reported to have positive effects on human health. In order to improve muscle fat composition from a human health standpoint, oilseeds, plant oils and marine oils can be used in ruminant diets. On the other hand, molecular mechanisms play an important role in the alteration of the FA composition of muscle fat. Genetics offer a wide range of possibilities for improvement of muscle fat composition by identifying different loci underlying the expression of quantitative traits. While significant progress has been made in characterizing the influence of diet on the FA composition of ruminant meat, the use of genetic tools can favor genotypes that could maximize their genetic potential through the diet. © 2016 Society of Chemical Industry.

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“…However, the transgenic goats were unable to sustain the altered fatty acid ratios possibly due to instabilities of the transgene-derived mRNA. Enabling livestock with the endogenous production of omega-3 fatty acids, which are highly beneficial for human health and mainly available through the consumption of oily fish, provides a new, more sustainable food source for omega-3 fatty acids and was demonstrated in pigs, sheep and cattle (Lai et al 2006;Saeki et al 2004;Zhang et al 2013;Cheng et al 2015). Transgenic cattle,…”

Section: Transfer Of Early Concepts From Mouse To Livestockmentioning

confidence: 99%

Improving milk for human consumption through genetic engineering technologies

Laible

Wagner

Wei

et al. 2017

NIB

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The opinions expressed and arguments employed in this publication are the sole responsibility of the authors and do not necessarily reflect those of the OECD or of the governments of its Member countries. The Conference was sponsored by the OECD Cooperative Research Programme on Biological Resource Management for Sustainable Agricultural Systems, whose financial support made it possible for some of the invited speakers to participate in the Conference.

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Production of transgenic beef cattle rich in n-3 PUFAs by somatic cell nuclear transfer

Abstract: Our results demonstrated that the codon-optimized C. elegans mfat1 gene can be functionally expressed in the beef cattle and converts n-6 PUFAs to n-3 PUFAs.

Cited by 15 publications

References 16 publications

Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep

Comparative Transcriptome Analysis Provides Insights into the Polyunsaturated Fatty Acid Synthesis Regulation of Fat-1 Transgenic Sheep

Impacts of fat from ruminants' meat on cardiovascular health and possible strategies to alter its lipid composition

Improving milk for human consumption through genetic engineering technologies

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