Production of Toxocara cati TES-120 Recombinant Antigen and Comparison with its T. canis Homolog for Serodiagnosis of Toxocariasis

Zahabiun, Farzaneh; Sadjjadi, Seyed Mahmoud; Yunus, Muhammad Hafiznur; Rahumatullah, Anizah; Moghaddam, Mohammad Hosein Falaki; Saidin, Syazwan; Noordin, Rahmah

doi:10.4269/ajtmh.15-0190

Cited by 19 publications

(8 citation statements)

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“…canis PCR-positive mice were also positive in the ELISA suggests a certain specificity. Recent attempts comparing recombinant ES antigens from both Toxocara species also showed considerable cross-reactivity [ 64 , 65 ] and initial experiments using monoclonal antibodies raised against T . canis antigens in a capture ELISA to detect circulating larval antigens showed high specificity (100%) but only low sensitivity (31%) to detect human toxocarosis [ 66 ].…”

Section: Discussionmentioning

confidence: 99%

Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany

et al. 2017

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Rodents are important intermediate and paratenic hosts for carnivore parasites, including the important zoonotic agents Toxoplasma, Echinococcus and Toxocara. Monitoring of such parasites in rodents can be used to detect increasing risks for human and veterinary public health. Rodents were trapped at four sites in Berlin, two near the city center, two at the periphery. PCRs were conducted to detect Coccidia (target ITS-1) and specifically Toxoplasma gondii (repetitive element) in brain and ascarids (ITS-2) in muscle or brain tissue. During necropsies, metacestodes were collected and identified using ITS-2 and 12S rRNA PCRs. An ELISA to detect antibodies against Toxocara canis ES antigens was performed. Within the 257 examined rodents, the most frequently observed parasite was Frenkelia glareoli predominantly found in Myodes glareolus. T. gondii was only detected in 12 rodents and Microtus spp. (although strongly underrepresented) had a significantly increased chance of being positive. Neither Echinococcus nor typical Taenia parasites of dogs and cats were found but Mesocestoides litteratus and Taenia martis metacestodes were identified which can cause severe peritoneal or ocular cysticercosis in dogs, primates and humans. Using PCR, the ascarids T. canis (n = 8), Toxocara cati (4) and Parascaris sp. (1) were detected predominantly in muscles. Seroprevalence of T. canis was 14.2% and ELISA was thus more sensitive than PCR to detect infection with this parasite. Non-parametric multidimensional scaling and cluster analysis revealed that parasite communities could be grouped into an urban and a peri-urban cluster with high frequency of ascarid-positive rodents in urban and high frequency of F. glareoli in peri-urban sites. Prevalence rates of parasites in rodents with potential impact for human or veterinary public health are considerable and the monitoring of transmission cycles of carnivore parasites in intermediate rodent hosts is recommended to estimate the health risks arising from wild and domesticated carnivores.

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Section: Discussionmentioning

confidence: 99%

Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany

et al. 2017

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“…Most studies that involve the use the recombinant antigens in toxocariasis diagnosis use both rTES‐30 and rTES‐120 as predicted models with high antigenic potential. Albeit human samples have shown a potential response for rTES‐30 and rTES‐120, as published by Mohamad et al (2009), Zahabiun et al (2015), Fong et al (2003) and Peixoto et al (2011), in mouse, the animal model for this disease, rTES‐120 did not prove itself as an antigenic protein (Figure ) . Sperotto et al (2017) published a proteomic analysis of in vitro T. canis larvae and identified both proteins, rTES‐30 and rTES‐120, indicating that larvae cultivated in vitro express them both .…”

Section: Resultsmentioning

confidence: 94%

“…In humans, recombinant proteins have been widely studied for toxocariasis diagnostic, with sensibility and specificity around 70%‐100% and 92%‐100%, respectively . By contrast, there is scarce information in animal studies, especially in mice, where the standard diagnostic methods are still utilized to study various infection paradigms, such as humoral immune response …”

Section: Introductionmentioning

confidence: 99%

Reactivity of recombinant Toxocara canis TES‐30/120 in experimentally infected mice

Santos

Moura

Azevedo

et al. 2018

Parasite Immunology

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“…It is important to state that we expect that the recombinant proteins have cross-reactivity with T . cati TES [9], homolog to T . canis , this cross-reactivity is not a specificity issue and it is important that both recombinant proteins detect T .…”

Section: Resultsmentioning

confidence: 99%

Sensitivity and specificity of recombinant proteins in Toxocara spp. for serodiagnosis in humans: Differences in adult and child populations

Santos¹,

Magalhães²,

Telmo³

et al. 2018

PLoS ONE

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Toxocariasis is a neglected zoonosis that affects children and adults. Recombinant proteins have been widely investigated for diagnosis, achieving high sensitivity and specificity in an overall population; however, little is known about age as a factor in its application. This study aims to investigate the diagnostic potential of Toxocara canis TES-30 and TES-120 recombinant proteins in humans, differentiating between its performance in children and adults. Serum samples collected from children and adults seropositive to Toxocara spp. were tested with indirect ELISA using T. canis TES-30 and TES-120 recombinant proteins produced in Escherichia coli. While rTES-30 sensitivity was not affected by age (81.8% in children and 87% in adults), rTES-120 sensitivity severely decreased in children to only 63.6%, down from 95.7% in adults. Furthermore, the sensitivity of rTES-30 increased to 97.8% after Western blotting confirmation. High specificity (>94%) against other geohelminths was reported for both recombinant proteins. Our study favors the use of rTES-30 with total IgG as the primary antibody in an indirect ELISA assay as a tool for epidemiological human studies.

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Production of Toxocara cati TES-120 Recombinant Antigen and Comparison with its T. canis Homolog for Serodiagnosis of Toxocariasis

Cited by 19 publications

References 19 publications

Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany

Small rodents as paratenic or intermediate hosts of carnivore parasites in Berlin, Germany

Reactivity of recombinant Toxocara canis TES‐30/120 in experimentally infected mice

Sensitivity and specificity of recombinant proteins in Toxocara spp. for serodiagnosis in humans: Differences in adult and child populations

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