Production of thermostable β-amylase by Clostridium thermosulfurogenes SV2 in solid-state fermentation:

Optimization of medium components using response surface methodology for production of thermostable amylopullulanase in submerged fermentation by Clostridium thermosulfurogenes SVM17

“…Five medium components, viz., soluble starch (0.5-6.5%), tapioca flour (0.5-6.5%), peptone (1.5-3.5%), MgCl2· 6H2O (0.005-0.025%) and FeSO4· 7H2O (2.0-10.0 ppm) with 3.5, 3.5, 2.5, 0.015 and 6.0 ppm, respectively as their central points under study were added to each vial as given in RSM experimental design (Table 1). The concentration range for each nutrient was fixed based on the literature (Rama Mohan Reddy et al, 2003) and on our own experience gained. The medium was flushed with nitrogen gas to create anaerobic conditions and vials were sealed and sterilized at 121 °C for 15 min.…”

“…The design contains a total of 54 experimental trials with first 32 organized in a fractional factorial design (Cochran and Cox, 1957;Rama Mohan Reddy et al, 2003;Mrudula, 2010), the experimental trials from 33-40 and 51-54 involve the replications of central points and experimental trials from 41-50 are axial points (star points). The response i.e., amount of enzyme produced by C. thermosulfurogenes SVM17 was assumed to be influenced by the five factors selected for the study.…”

“…A two fold increase in -amylase production was reported in B. circulans GRS313 (Dey et al, 2002) by use of response surface methodology. Rama Mohan Reddy et al, (1999;2003) reported about 10 and 36% more pullulanase and -amylase by Clostridium thermosulfurogens SV2 after optimization of medium composition.…”