Production of thermostable α-galactosidase from thermophilic fungusHumicola sp

Multiple α-galactosidases from Aspergillus foetidus ZU-G1: purification, characterization and application in soybean milk hydrolysis

“…For -gal II, melibiose and stachyose showed best aYnity than raYnose. The K m values determined for pNPG were not all lower than natural substrates, such as raYnose, meliboise and stachyose, and the results were diVer with other reports [1,30]. From V max /K m values, we can see the catalytic power of -gal I, -gal II and -gal III to raYnose was few diVerence and the same to pNPG.…”

“…The optimum temperature was determined by incubating enzyme samples in 0.2 M McIlvaine buVer pH5.0 at each temperature (30,35,40,45, 50, 55, 60 and 65°C), and temperature stability was also determined by incubating enzyme samples over a same temperature range for the period of 30 and 60 min. Aliquots were withdrawn after suitable time interval and residual enzyme activity was determined by standard assay method.…”

High-Yield Production of Alpha-Galactosidase Excreted fromPenicillium ChrysogenumandAspergillus Niger

“…5) the biomass concentration was reduced almost twice from its maximal value of 12.1 g/l in the 48 th h to 5.6 g/l in the 120 th h. Maximum α-galactosidase activity occurred at ph 4.8, also in the absence of ph control. the best enzyme yields of α-galactosidase by P. chrysogenum ln 33 and A. niger M 47 were several-fold higher than the activities reported for other fungi belonging to the genera Aspergillus, Penicillium, Thermomyces, Humicola and Trichoderma (1,4,7,8,9,11,16,19,26,30).…”

“…the enzymic conversion of raffinose family oligosaccharides (RO) in soymilk may be a rational alternative to improve the nutritional quality of this low-cost, high-quality protein supplement for humans and animals (20). in the pulp and paper industry α-galactosidase could enhance the bleaching effect of β-mannanases on softwood pulp (7). In human medicine this enzyme can be used for the treatment of Fabry's disease (3) or for the blood type conversion (25).…”

Soy Meal Waste Extract as Cultivation Medium for Production of Extracellular α-Galactosidase from the FungusHumicola Lutea120–5

“…α-Galactosidases have an wide application in biotechnology for the nutritional improvement of legumes based foods and fodders (13). Several fungal genera have been studied preferably for production of extracellular α-galactosidases: Aspergillus (1, 9, 13), Penicillium (8), Thermomyces (11,12) and Humicola (6,7). The fermentations have been carried out on media containing mono-and oligosaccharadides (13); galactomannan (1); wheat arabinoxylan (9) as well as waste cakes from the oats, coconut, wheat and soy processing, locust bean gum, out-husk meal (7,8).…”