Production of Thermostable Organic Solvent Tolerant Keratinolytic Protease from Thermoactinomyces sp. RM4: IAA Production and Plant Growth Promotion

Abstract

There are several reports about the optimization of protease production, but only few have optimized the production of organic solvent tolerant keratinolytic proteases that show remarkable exploitation in the development of the non-polluting processes in biotechnological industries. The present study was carried with aim to optimize the production of a thermostable organic solvent tolerant keratinolytic protease Thermoactinomyces sp. RM4 utilizing chicken feathers. Thermoactinomyces sp. RM4 isolated from the soil sample collected from a rice mill wasteyard site near Kashipur, Uttrakhand was identified on the basis of 16S rDNA analysis. The production of organic solvent tolerant keratinolytic protease enzyme by Thermoactinomyces sp. RM4 was optimized by varying physical culture conditions such as pH (10.0), temperature (60°C), inoculum percentage (2%), feather concentration (2%) and agitation rate (2 g) for feather degradation. The result showed that Thermoactinomyces sp. RM4 potentially produces extra-cellular thermostable organic solvent tolerant keratinolytic protease in the culture medium. Further, the feather hydrolysate from keratinase production media showed plant growth promoting activity by producing indole-3-acetic acid itself. The present findings suggest that keratinolytic protease from Thermoactinomyces sp. RM4 offers enormous industrial applications due to its organic solvent tolerant property in peptide synthesis, practical role in feather degradation and potential function in plant growth promoting activity, which might be a superior candidate to keep ecosystem healthy and functional.

Co-culture development and bioformulation efficacy of psychrotrophic PGPRs to promote growth and development of Pea (<i>Pisum sativum</i>) plant

“…These IAA production data suggest that the isolated strains act as effective bioinoculants. These trends agree with previous reports indicating IAA formation in the stationary stage of culture (Park et al, 2017;Sahasrabudhe, 2011;Verma et al, 2016;Walpola et al, 2013). The confirmation of IAA with the TLC findings are in agreement with previous reports (Sahasrabudhe, 2011).…”

“…Furthermore, to quantify the IAA produce in the tubes, the absorbance was measured at 530 nm. The concentration of IAA in each bacterial strain was determined and quantified by comparing with a standard curve of IAA (Verma et al, 2016).…”

“…temperature, moisture, salt concentration, chemical composition, as well as the plant community, influences the bacterial community found in different soils. The bacteria that can promote plant growth are called plant growth promoting rhizobacteria (PGPR) (Verma et al, 2016). They endorse plant growth either directly by facilitating resource attainment of plant hormone levels, or indirectly by lessening the inhibitory property of various pathogenic agents on plant growth and development by acting as a biocontrol agent (Anwar et al, 2014).…”

Production of Alkaline Keratinolytic Protease by Bacillus sp. B13 from Feather Waste

“…Similar result was also found for Streptomyces pulvereceus MTCC 8374 by Jayasree et al, (2009 Verma et al, 2016 It is well documented that an inoculum size of 2% to 5% is optimum for protease production (Mabrouk et al, 1999;Kanekar et al, 2002).Moreover, in the reports of Sinha and Satyanarayana (1991) and according to Gajju et al, (1996) range of 1% to 8% inoculums was the optimum.…”

Production and Characterization of Keratinolytic Proteases by a Chicken Feather-Degrading Thermophilic Strain, Thermoactinomyces sp. YT06

“…Some Thermoactinomyces spp. [3,17] are known to degrade keratins. We had previously screened and isolated a feather-degrading strain from high-temperature poultry compost in Nanjing, China, and based on its morphology and 16S rRNA identification, the isolate was tentatively named Thermoactinomyces sp.…”