Production of the amino acids l-glutamate, l-lysine, l-ornithine and l-arginine from arabinose by recombinant Corynebacterium glutamicum

Harnessing novel chromosomal integration loci to utilize an organosolv‐derived hemicellulose fraction for isobutanol production with engineered Corynebacterium glutamicum

“…We designed homologous flanking regions of > 500 bps to specifically locate the additional genetic information to designated CgLPs. The two synthetic operons express the xylAB genes encoding XylA (xylose isomerase) of Xanthomonas campestris and XylB (xylulokinase) of C. glutamicum and araBAD encoding AraB ( l ‐ribulokinase), AraA ( l ‐arabinose isomerase) and AraD ( l ‐ribulose‐5‐phosphate 4‐epimerase) of E. coli MG1655 under control of the constitutive promoter of the C. glutamicum elongation factor EF‐TU (cg0587, P tuf ) and are terminated by the E. coli rrnB operon terminator (T rrnB ) respectively, following already published operon architectures (Schneider et al ., 2011; Meiswinkel et al ., 2013). …”

“…2B, C). Previous studies using plasmid‐based expression of araBAD (Schneider et al ., 2011) or xylAB (Meiswinkel et al ., 2013) yielded maximal rates of 0.31 h −1 or 0.20 h −1 respectively. In our experiments, a full consumption of the pentoses was not achieved at the end of cultivation (78 ± 7% of d ‐xylose and 14 ± 4% of l ‐arabinose metabolized).…”

“…In our experiments, a full consumption of the pentoses was not achieved at the end of cultivation (78 ± 7% of d ‐xylose and 14 ± 4% of l ‐arabinose metabolized). Poor l ‐arabinose uptake can be explained by a high Monod constant (Schneider et al ., 2011) and could be overcome by additional expression of the transporter araE , which was shown to also improve d ‐xylose consumption (Sasaki et al ., 2009). Combined supplementation of d ‐glucose, d ‐xylose and l ‐arabinose showed a clear preference for the consumption of the hexose compared to the pentoses (cf.…”

Ratiometric fluorescent l‐arginine and l‐asparagine biosensors based on the oxazine 170 perchlorate‐ethyl cellulose membrane

“…Artificial urine solution was prepared by mixing 2 mM citric acid, 1.1 mM lactic acid, 90 mM NaCl, 25 mM NH 4 …”

“…From the high immobilization efficiency of urease (20 U) the immobilization efficiency of only arginase into the EC matrix was estimated to be ࣙ85%. However, too large amounts of arginase immobilized into the EC matrix may hinder the NH 4 + transport to contact with the O17-EC membrane. The high density of the enzyme supporting layer led to the difficult passage of NH 4 + to react with the O17-EC membrane below the enzyme layer, whereas the lower amounts of arginase decreased the sensitivity of the O17-EC membrane.…”