Production of tailor‐made fructans in sugar beet by expression of onion fructosyltransferase genes

Weyens, Guy; Ritsema, Tita; Dun, Kees van; Meyer, Diederick; Lommel, M.; Lathouwers, J.; Rosquin, I.; Denys, Pierre; Tossens, A.; Nijs, Marleen; Turk, Stefan C. H. J.; Gerrits, Nathalie; Bink, Sjak; Walraven, Bas; Lefebvre, Marc; Smeekens, Sjef

doi:10.1111/j.1467-7652.2004.00074.x

Cited by 62 publications

(26 citation statements)

References 24 publications

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“…In sugar beet, introduction of an enzyme of fructan synthesis from onion resulted in substantial conversion of the sucrose content of the storage root to fructans of low molecular mass (Weyens et al, 2004). However, this apparently did not result in greater import of sucrose into the roots: total carbohydrate content was not affected.…”

Section: Increasing Sucrose Contentmentioning

confidence: 99%

Prospects for increasing starch and sucrose yields for bioethanol production

Smith

2008

The Plant Journal

156

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SummaryIn the short term, the production of bioethanol as a liquid transport fuel is almost entirely dependent on starch and sugars from existing food crops. The sustainability of this industry would be enhanced by increases in the yield of starch/sugar per hectare without further inputs into the crops concerned. Efforts to achieve increased yields of starch over the last three decades, in particular via manipulation of the enzyme ADPglucose pyrophosphorylase, have met with limited success. Other approaches have included manipulation of carbon partitioning within storage organs in favour of starch synthesis, and attempts to manipulate source-sink relationships. Some of the most promising results so far have come from manipulations that increase the availability of ATP for starch synthesis. Future options for achieving increased starch contents could include manipulation of starch degradation in organs in which starch turnover is occurring, and introduction of starch synthesis into the cytosol. Sucrose accumulation is much less well understood than starch synthesis, but recent results from research on sugar cane suggest that total sugar content can be greatly increased by conversion of sucrose into a non-metabolizable isomer. A better understanding of carbohydrate storage and turnover in relation to carbon assimilation and plant growth is required, both for improvement of starch and sugar crops and for attempts to increase biomass production in second-generation biofuel crops.

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Section: Increasing Sucrose Contentmentioning

confidence: 99%

Prospects for increasing starch and sucrose yields for bioethanol production

Smith

2008

The Plant Journal

156

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“…This makes the Mll promoter a very good candidate for biotechnological applications. For example, a higher tissue specific activity of introduced fructosyltransferases may be achieved in roots to convert sucrose to low molecular fructans (Weyens et al 2004) The promoter reporter gene fusions transformed into tobacco also show a specific expression profile. The expression of the Mll and Tlp promoters in root and hypocotyl support the conclusion that storage root expressed genes require regulatory elements for these tissues because the beet root comprises hypocotyl and primary root tissue (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…For example, the introduced Helianthus tuberosus 1-sucrose:sucrose fructosyl transferase converted almost all sucrose in the storage root of sugar beet to low molecular fructans (Sevenier et al 1998). A different approach achieved the conversion of sucrose to onion-type fructans by introducing onion fructosyltransferases under the control of the Arabidopsis ubiquitin 3 promoter into transgenic sugar beets (Weyens et al 2004). Fructosyltransferase activity was detected in leaf and root tissue.…”

Section: Introductionmentioning

confidence: 99%

Taproot promoters cause tissue specific gene expression within the storage root of sugar beet

Oltmanns¹,

Kloos²,

Brieß³

et al. 2006

Planta

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The storage root (taproot) of sugar beet (Beta vulgaris L.) originates from hypocotyl and primary root and contains many different tissues such as central xylem, primary and secondary cambium, secondary xylem and phloem, and parenchyma. It was the aim of this work to characterize the promoters of three taproot-expressed genes with respect to their tissue specificity. To investigate this, promoters for the genes Tlp, His1-r, and Mll were cloned from sugar beet, linked to reporter genes and transformed into sugar beet and tobacco. Reporter gene expression analysis in transgenic sugar beet plants revealed that all three promoters are active in the storage root. Expression in storage root tissues is either restricted to the vascular zone (Tlp, His1-r) or is observed in the whole organ (Mll). The Mll gene is highly organ specific throughout different developmental stages of the sugar beet. In tobacco, the Tlp and Mll promoters drive reporter gene expression preferentially in hypocotyl and roots. The properties of the Mll promoter may be advantageous for the modification of sucrose metabolism in storage roots.

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“…Previously, it has been reported that expression of plant fructosyltransferase transgenes in variety of transgenic plants induces the synthesis of fructans and fructooligosaccharides or increases their contents (Hellwege et al 1997;Hisano et al 2004;Jenkins et al 2002;Kawakami et al 2008;Ritsema and Smeekens, 2003;Sévenier et al 1998;Stoop et al 2007;Weyens et al 2004). Successful accumulation of bacterial fructan (levan; β-2,6-linked fructan) has been also achieved by the introduction of bacterial levan sucrase into transgenic plants (reviewed by Cairns 2003).…”

Section: High Performance Liquid Chromatography (Hplc) Analysesmentioning

confidence: 99%

High-level fructooligosaccharide production in transgenic tobacco plants

Fukutomi

Yoshinaka

Kawamoto

et al. 2013

Plant Biotechnology

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β-fructofuranosidase (FFase) of Aspergillus niger ATCC 20611 can transfer fructosyl residues from one sucrose to another for the synthesis of glucose and fructooligosaccharides composed of 1-kestose (GF 2 ), nystose (GF 3 ), and β-fructofuranosylnystose (GF 4 ). The FFase gene, under the control of the sporamin gene promoter from sweet potato, was introduced into tobacco plants. Sporamin promoter activity is induced by sugar and exhibits preferential expression in stem and root tissues. Thin-layer and high performance liquid chromatographic analyses showed that soluble extracts from the transgenic plants contained considerable amounts of fructooligosaccharides such as GF 2 and GF 3 . The conversion of sucrose into fructooligosaccharides did not affect plant growth or development. Our results indicate that the transgenic plants could be utilized as bioreactors, and this opens up the possibility for efficient production of fructooligosaccharides in sucroseproducing plants such as sugar beet and sugarcane.

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Production of tailor‐made fructans in sugar beet by expression of onion fructosyltransferase genes

Cited by 62 publications

References 24 publications

Prospects for increasing starch and sucrose yields for bioethanol production

Prospects for increasing starch and sucrose yields for bioethanol production

Taproot promoters cause tissue specific gene expression within the storage root of sugar beet

High-level fructooligosaccharide production in transgenic tobacco plants

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