Production of Sporangia by Phytophthora Cinnamomi in Axenic Culture

Chen, Dah-wu; Zentmyer, G. A.

doi:10.1080/00275514.1970.12018977

Cited by 96 publications

(42 citation statements)

References 3 publications

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“…The squares were placed in 90-mm plastic culture dishes with approximately 20 ml of V8 broth (Chen and Zentmyer, 1970) and grown for 5 days at 25°C in the dark. The V8 broth was changed every day.…”

Section: Phytophthora Strain and Culture Conditionsmentioning

confidence: 99%

“…Vegetative hyphae were grown from seven small squares (3 ϫ 3 mm) of mycelium cut from the edge of a colony grown on V8 nutrient agar (Chen and Zentmyer, 1970). The squares were placed in 90-mm plastic culture dishes with approximately 20 ml of V8 broth (Chen and Zentmyer, 1970) and grown for 5 days at 25°C in the dark.…”

Section: Phytophthora Strain and Culture Conditionsmentioning

confidence: 99%

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The Role of Proline in Osmoregulation in Phytophthora nicotianae

Ambikapathy

Marshall

Hocart

et al. 2002

Fungal Genetics and Biology

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Section: Phytophthora Strain and Culture Conditionsmentioning

confidence: 99%

Section: Phytophthora Strain and Culture Conditionsmentioning

confidence: 99%

The Role of Proline in Osmoregulation in Phytophthora nicotianae

Ambikapathy

Marshall

Hocart

et al. 2002

Fungal Genetics and Biology

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“…For example, P. cinnamomi suggests using nonsterile soil extract, incubating the isolates under fluorescent white light and performing fluid changes until sporangia are observed (Chen and Zentmyer, 1970;Eye et al, 1978;Ahumada et al, 2013).…”

Section: Identificación Molecularmentioning

confidence: 99%

Protocolos de aislamiento y diagnóstico de Phytophthora Spp.: enfoque aplicado a la investigación

Plancarte

Alvarado

Pavía

et al. 2017

Remexca

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ResumenEl propósito de esta revisión es dar a conocer al investigador, protocolos fundamentales que se emplean para el aislamiento, diagnóstico e identificación de Phytophthora spp., cubriendo aspectos generales del género para una mejor comprensión y análisis del mismo. Esta información será de utilidad para investigadores que trabajan con enfermedades causadas por este oomicete, y va dirigida a países de América Latina. Actualmente, se emplean métodos convencionales y moleculares para la detección e identificación de especies de Phytophthora. Los métodos convencionales incluyen el uso de claves taxonómicas, la observación de síntomas y detección de signos del patógeno en las plantas enfermas y el aislamiento del agente causal del tejido vegetal infectado, del suelo que rodea a las raíces y de las fuentes de agua utilizadas para el riego, usando medios de cultivo generales o selectivos. Los métodos moleculares proporcionan resultados que son más precisos en la identificación de Phytophthora, además de que se pueden realizar en lapsos de tiempo más cortos y no es indispensable que el investigador tenga experiencia en AbstractThe purpose of this review is to make known to the researcher, fundamental protocols that are used for the isolation, diagnosis and identification of Phytophthora spp., covering general aspects of the genus for a better understanding and analysis of the same. This information will be useful for researchers working with diseases caused by this oomycete, and is mainly addressed to countries in Latin America. Currently, conventional and molecular methods are used for the detection and identification of Phytophthora species. Conventional methods include the use of taxonomic keys, observation of symptoms and detection of signs of the pathogen in diseased plants, and isolation of the causative agent of infected plant tissue, soil surrounding roots and water sources used for irrigation, using general or selective culture media. Molecular methods provide results that are more accurate in Phytophthora identification and can be performed in shorter time periods and it is not essential that the researcher has experience in aspects of the morphology of members

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“…Sporangial production and release of zoospores into liquid media was achieved using a modified mycelial matt method (Chen & Zentmyer 1970). Agar blocks bearing mycelium were cut from the edges of colonies and transferred into 20 ml clarified V8 juice broth and incubated at room temperature for 4 d.…”

Section: Morphological Cultural and Physiological Characteristicsmentioning

confidence: 99%

Two new Phytophthora species from South African Eucalyptus plantations

Maseko

Burgess

Coutinho

et al. 2007

Mycological Research

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A recent study to determine the cause of collar and root rot disease outbreaks of cold tolerant Eucalyptus species in South Africa resulted in the isolation of two putative new Phytophthora species. Based on phylogenetic comparisons using the ITS and β-tubulin gene regions, these species were shown to be distinct from known species. These differences were also supported by robust morphological characteristics. The names, Phytophthora frigida sp. nov. and Phytophthora alticola sp. nov. are thus provided for these taxa, which are phylogenetically closely related to species within the ITS clade 2 (P. citricola, P. tropicali and P.multivesiculata) and 4 (P. arecae and P. megakarya), respectively. Phytophthora frigida is heterothallic, and produces stellate to rosaceous growth patterns on growth medium, corraloid hyphae, sporangia with a variety of distorted shapes and has the ability to grow at low temperatures. Phytophthora alticola is homothallic and has a slower growth rate in culture. Both P. frigida and P. alticola are pathogenic to Eucalyptus dunnii. In pathogenicity tests, they were, however, less pathogenic than P. cinnamomi, which is a well-known pathogen of Eucalyptus in South Africa.

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Production of Sporangia by Phytophthora Cinnamomi in Axenic Culture

Cited by 96 publications

References 3 publications

The Role of Proline in Osmoregulation in Phytophthora nicotianae

The Role of Proline in Osmoregulation in Phytophthora nicotianae

Protocolos de aislamiento y diagnóstico de Phytophthora Spp.: enfoque aplicado a la investigación

Two new Phytophthora species from South African Eucalyptus plantations

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