Production of Sophorolipid Biosurfactant byPichia anomala

Thaniyavarn, Jiraporn; Chianguthai, Tanusta; Sangvanich, Polkit; Roongsawang, Niran; Washio, Keji; Morikawa, Masaaki; Thaniyavarn, Suthep

doi:10.1271/bbb.80166

Cited by 89 publications

(48 citation statements)

References 21 publications

(37 reference statements)

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“…A select number of yeast species, such as Candida apicola (Gorin et al, 1961), Candida bogoriensis (Tulloch et al, 1968), Wickerhamiella domercqiae (Chen et al 2006a), Pichia anomala (Thaniyavarn et al, 2008), Candida guilliermondii (Sitohy et al, 2010), and Candida bombicola (Spencer et al 1970, Daverey and Pakshirajan, 2009, Parekh et al, 2012 etc., were reported to produce extracellular biosurfactant as SLs.…”

Section: Introductionmentioning

confidence: 99%

Production, characterization and anticancer activity of Candida bombicola sophorolipids by means of solid state fermentation of sunflower oil cake and soybean oil

Rashad¹,

Nooman²,

Ali³

et al. 2014

Grasas y Aceites

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SUMMARY:The production of sophorolipids by Candida bombicola NRRL Y-17069 grown in a mixture of sunflower oil cake and crude soybean oil as economic substrates with different fermentation techniques was studied. The highest yield (49.5 g·100 g −1 substrates) was obtained from solid state fermentation after employing a new concept for extraction by methanol (E I) followed by ethyl acetate (E II), then partially purified with hexane (E III). The course of time of fermentation was also studied, and E I extracted of the 12 th day showed the minimum surface tension (45 mN·m −1 ) at a critical micelle dilution (CMD) of 10% concentration. The produced sophorolipids were characterized and confirmed by FTIR and 1 H NMR spectroscopy. The anticancer activity of the produced compounds was assessed against MCF-7, HepG2, A549, HCT116 cancer cell lines and the results revealed that E III and E IV (a mixture of E I & E III) act as promising anticancer agents in HepG2 and A549 by inhibiting urokinase and histone deacetylase activities. KEYWORDS: Anticancer activity; Candida bombicola; Solid state fermentation; Sophorolipids; Soybean oil; Sunflower oilcakeRESUMEN: Producción, caracterización y actividad anticancerígena de soforolípidos producidos mediante fermentación en estado sólido con Candida bombicola de tortas de girasol y aceite de soja. Se estudió la producción de soforolípidos por Candida bombicola NRRL Y-17069 cultiva con diferentes técnicas de fermentación en una mezcla de torta de girasol y aceite de soja crudo, como sustratos económicos. El rendimiento más alto (49,5 g·100 g −1 de sustrato) se obtuvo por fermentación en estado sólido después de extraer con metanol (IE) seguido de acetato de etilo (EII), y de purificación parcial con hexano (EIII). También se estudió el tiempo de fermentación, considerando que el extracto IE de 12 días mostró una tensión superficial mínima (45 mN·m −1 ) a una dilución micelar crítica (CMD) de concentración 10 %. Los soforolípidos producidos se caracterizaron y se confirmaron mediante espectroscopia FTIR y RMN de 1 H. La actividad anticancerígena de los compuestos producidos se evaluó en células MCF-7, HepG2, A549, líneas celulares de cáncer de HCT116 y los resultados revelaron que EIII y EIV (una mezcla de EI y EIII) actúan como prometedores agentes anticancerígenos en HepG2 y A549 inhibiendo las actividades de uroquinasa e histona desacetilasa.

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Section: Introductionmentioning

confidence: 99%

Production, characterization and anticancer activity of Candida bombicola sophorolipids by means of solid state fermentation of sunflower oil cake and soybean oil

Rashad¹,

Nooman²,

Ali³

et al. 2014

Grasas y Aceites

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“…10) We examined biosurfactant productivity in various 2% carbon sources, including glucose, glycerol, palm oil, and soybean oil, or a mixture of glucose and glycerol, palm oil, or soybean oil (Table 1). Strain JP52 showed maximum cell growth on day 3 of all the tested media (not shown).…”

Section: Resultsmentioning

confidence: 99%

“…10) Within the Starmerrella yeast clade, S. bombicola, C. apicola, Candida riodocensis, Candida stellata, and Candida sp. NRRL Y-27208 have been reported to show significant sophorolipid productivity.…”

Section: )mentioning

confidence: 99%

“…The relative mobility (Rf) values of the positive fractions were recorded, and then the fractions were harvested, dissolved in ethyl acetate, and assayed for carbohydrate components by the Molish test, and oil displacement activity, as previously reported. 10) Positive fractions with high oil displacement activity were further analyzed by matrix-assisted laser desorption/ ionization-time of flight mass spectrometry (MALDI-TOF-MS) to identify the glycolipid type in terms of a sophorolipid, mannosyl erythritol lipid, or rhamnolipid, as reported previously. 9,20,21) MALDI-TOF-MS analysis was performed at the Equipment Management Center, Creative Research Institution, Hokkaido University, as previously described 11,21) on Voyager-DE STR-H operating in linear mode with positive ion detection (Bruker Daltonics, Billerica, MA).…”

Section: Purification and Chemical Structure Analysis Of The Biosurfamentioning

confidence: 99%

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Production and Characterization of a Biosurfactant fromCyberlindnera samutprakarnensisJP52^T

Poomtien

Thaniyavarn

Pinphanichakarn

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…We have previously reported that W. anomalus PY1, a thermo-tolerant strain isolated from fermented food, is a sophorolipid producer which can produce sophorolipids in a wide range of temperature (20-40°C) 10 . Enhanced production of sophorolipids by PY1 or its derivative strains is thus expected to be useful for improvement of preservation and value of foods.…”

Section: Introductionmentioning

confidence: 99%

Production of biosurfactant by Wickerhamomyces anomalus PY189 and its application in lemongrass oil encapsulation

Dejwatthanakomol¹,

Anuntagool²,

Morikawa³

et al. 2016

ScienceAsia

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ABSTRACT:In this study, the production and characterization of a biosurfactant from yeast Wickerhamomyces anomalus strain PY189 was carried out. The highest efficiency for biosurfactant production was found when the organism was grown in a medium containing 4% (v/v) soya bean oil and 0.4% (w/v) NaNO 3 at 30°C and pH 5.5 for 7 days. After 7 days of cultivation, W. anomalus PY189 was able to produce up to 0.57 g/l of biosurfactant as ethyl acetate extracts. The culture supernatant was able to reduce the surface tension of the culture broth from 42.5 mN/m to 36.5 mN/m with a critical micelle concentration of 204 mg/l. The crude extract of biosurfactants was then applied to the encapsulation of lemongrass oil. Emulsions of lemongrass oil in 20 mg/dl maltodextrin solution (oil:maltodextrin solution ratios of 0.2:1, 0.15:1, and 0.1:1) containing 0.8% and 1% (w/v) crude biosurfactant extract were stable for at least 24 h and had an average oil droplet size of less than 10 µm. Lemongrass oil microcapsules were later produced using a spray drying technique. This microcapsule exhibited microbial growth inhibition activity against E. coli, S. aureus, and Salmonella at 5% (w/v) concentration.

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Production of Sophorolipid Biosurfactant byPichia anomala

Cited by 89 publications

References 21 publications

Production, characterization and anticancer activity of <em>Candida bombicola</em> sophorolipids by means of solid state fermentation of sunflower oil cake and soybean oil

Production, characterization and anticancer activity of <em>Candida bombicola</em> sophorolipids by means of solid state fermentation of sunflower oil cake and soybean oil

Production and Characterization of a Biosurfactant fromCyberlindnera samutprakarnensisJP52^T

Production of biosurfactant by Wickerhamomyces anomalus PY189 and its application in lemongrass oil encapsulation

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Production of Sophorolipid Biosurfactant byPichia anomala

Cited by 89 publications

References 21 publications

Production, characterization and anticancer activity of <em>Candida bombicola</em> sophorolipids by means of solid state fermentation of sunflower oil cake and soybean oil

Production, characterization and anticancer activity of <em>Candida bombicola</em> sophorolipids by means of solid state fermentation of sunflower oil cake and soybean oil

Production and Characterization of a Biosurfactant fromCyberlindnera samutprakarnensisJP52T

Production of biosurfactant by Wickerhamomyces anomalus PY189 and its application in lemongrass oil encapsulation

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Production and Characterization of a Biosurfactant fromCyberlindnera samutprakarnensisJP52^T