Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants

Yip, Mei-Kuen; Huang, Heng‐Tsung Danny; Ger, Mang‐Jye; Chiu, Shih-Hua; Tsai, Yuh-Chih; Lin, Chia-Wei; Feng, Tao

doi:10.1007/s00299-006-0246-y

Cited by 32 publications

(19 citation statements)

References 29 publications

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“…Transgenic expression of PFLP from sweet pepper in calla lily resulted in more resistance to soft rot bacterial diseases. 769 The same transformation in tobacco, orchid, and rice plants enhanced their resistance to Xanthomonas oryzae pv. oryzae .…”

Section: Fe–s Redox Centers In Electron Transfer Processesmentioning

confidence: 99%

Metalloproteins Containing Cytochrome, Iron–Sulfur, or Copper Redox Centers

et al. 2014

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Blue Type I Copper Centers vs Purple Cu A Centers 4439 5. Enzymes Employing a Combination of Different Types of Electron Transfer Centers 4439 5.1. Enzymes Using Both Heme and Cu as Electron Transfer Centers 4439 5.1.1. Cytochrome c and Cu A as Redox Partners to Cytochrome c Oxidases 4439 5.1.2. Cu A and Heme b as Redox Partners to Nitric Oxide Reductases 4440 5.1.3. Cytochrome c and Cu A as Redox Partners to Nitrous Oxide Reductases 4440 5.2. Enzymes Using Both Heme and Iron−Sulfur Clusters as Electron Transfer Centers 4440 5.2.1. As Redox Partners to the Cytochrome bc 1 Complex 4440 5.2.2. As Redox Partners to the Cytochrome b 6 f Complex 4442 5.2.3. As Redox Centers in Formate Dehydrogenases 4443 5.2.4. As Redox Centers in Nitrate Reductase 4443 6. Summary and Outlook 4443 Author Information 4445 Corresponding Author 4445 Author Contributions 4445 Notes 4445 Biographies 4445 Acknowledgments 4447 Abbreviations 4447 References 4447

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Section: Fe–s Redox Centers In Electron Transfer Processesmentioning

confidence: 99%

Metalloproteins Containing Cytochrome, Iron–Sulfur, or Copper Redox Centers

et al. 2014

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“…Cefotaxime concentration used to inhibit Agrobacterium tumefaciens growth in this study was the same as reported in a previous study (Yip et al 2007). After 2 days of cocultivation, the discs were then transferred onto the regeneration medium (BI medium containing 500 mg/l cefotaxime) and incubated at 26 ± 2°C with a 16-h photoperiod (60 lmol m -2 s -2 ) under cool white fluorescent lamps for 3 weeks.…”

Section: Growth and Recovery Of The Transgenic Plantsmentioning

confidence: 91%

“…This peptide has been reported to enhance resistance against bacterial pathogens such as Xanthomonas oryzae pv oryzae in rice (Tang et al 2001); Erwinia carotovora subsp. carotovora in transgenic Oncidium orchid Liau et al 2003); tobacco (Huang et al 2006) and calla lily (Yip et al 2007); Pseudomonas spp. in tobacco (Lin et al 1997;Huang et al 2004) and Ralstonia solanacearum in tomato .…”

Section: Introductionmentioning

confidence: 99%

An easy and efficient protocol in the production of pflp transgenic banana against Fusarium wilt

Yip

Lee

et al. 2011

Plant Biotechnol Rep

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This study describes an efficient protocol for Agrobacterium tumefaciens-mediated transformation of two subgroups of genotype AAA bananas (Musa acuminata cv. Pei Chiao and Musa acuminata cv. Gros Michel). Instead of using suspension cells, cauliflower-like bud clumps, also known as multiple bud clumps (MBC), were induced from sucker buds on MS medium containing N 6 -Benzylaminopurine (BA), Thidiazuron (TDZ), and Paclobutrazol (PP333). Bud slices were co-cultivated with A. tumefaciens C58C1 or EHA105 that carry a plasmid containing Arabidopsis root-type ferredoxin gene (Atfd3) and a plant ferredoxin-like protein (pflp) gene, respectively. These two strains showed differences in transformation efficiency. The EHA105 strain was more sensitive in Pei Chiao, 51.3% bud slices were pflp-transformed, and 12.6% slices were Atfd3-transformed. Gros Michel was susceptible to C58C1 and the transformation efficiency is 4.4% for pflp and 13.1% for Atfd3. Additionally, gene integration of the putative pflp was confirmed by Southern blot. Resulting from the pathogen inoculation assay, we found that the pflp transgenic banana exhibited resistance to Fusarium oxysporum f. sp. cubense tropical race 4. This protocol is highly advantageous to banana cultivars that have difficulties in setting up suspension cultures for the purpose of quality improvement through genetic transformation. In addition, this protocol would save at least 6 months in obtaining explants for transformation and reduce labor for weekly subculture in embryogenic cell suspension culture systems.

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“…It was reported that commercial hybrids of calla lily could partly escape from P. carotovorum infection within numberable years and the transgenic calla lily with ferredoxin-like protein gene (pflp) was relatively resistant to P. carotovorum subsp. carotovorum (Yip et al 2007). However, few reports on the effective control of this serious plant disease were documented.…”

Section: Introductionmentioning

confidence: 97%

“…Bacterial soft rot caused by P. carotovorum subsp. carotovorum is a serious plant disease in calla lily, and responsible for considerable losses in the industry of this plant (Snijder et al 2004;Yip et al 2007). It was reported that commercial hybrids of calla lily could partly escape from P. carotovorum infection within numberable years and the transgenic calla lily with ferredoxin-like protein gene (pflp) was relatively resistant to P. carotovorum subsp.…”

Section: Introductionmentioning

confidence: 99%

Biocontrol of bacterial soft rot of calla lily by elicitor HarpinXoo and N-acyl homoserine lactonase (AttM)

Fan

Qian

Yang

et al. 2010

World J Microbiol Biotechnol

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Bacterial soft rot caused by Pectobacterium carotovorum subsp. carotovorum is a serious plant disease in Zantedeschia spp. (also called calla lily). In this study, two independent genes (a N-acyl homoserine lactonase gene attM from Agrobacterium tumefaciens and a hypersensitive response and pathogenicity gene hrf1 from Xanthomonas oryzae pv. oryzae), transcribed by a strong and constitutive Escherichia coli promoter P lpp , respectively, were cloned into plasmid pUC19, and was transformed into E. coli, creating strain JM109/pPHA. The result of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) assay showed that both genes (hrf1 and attM) were successfully expressed in one plasmid system in strain JM109/pPHA. The expressed Harpin Xoo (Hrf1) and AttM protein had the ability of inducing hypersensitive response (HR) in nonhost tobacco and degrading the N-acyl homoserine lactones (AHLs) produced by P. carotovorum subsp. carotovorum, respectively, whereas Harpin Xoo and AttM protein did not seem to interfere with the normal growth of this pathogen. In planta, strain JM109/pPHA could significantly reduce the soft rot disease severity on dormant tubers (control efficiency: 92.8%) or potted plants (control efficiency: 92.4%) of calla lily. We have first demonstrated the both biocontrol effects of Harpin Xoo and AttM proteins (also described as Quorum interference) on the bacterial soft rot disease of calla lily, caused by P. carotovorum subsp. carotovorum. This work provided a potential way to control this serious plant disease.

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Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants

Cited by 32 publications

References 29 publications

Metalloproteins Containing Cytochrome, Iron–Sulfur, or Copper Redox Centers

Metalloproteins Containing Cytochrome, Iron–Sulfur, or Copper Redox Centers

An easy and efficient protocol in the production of pflp transgenic banana against Fusarium wilt

Biocontrol of bacterial soft rot of calla lily by elicitor HarpinXoo and N-acyl homoserine lactonase (AttM)

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