Production of Siderophores by Serratia marcescens and the Role of MacAB Efflux Pump in Siderophores Secretion

Khilyas, Irina V.; Shirshikova, Tatiana V.; Matrosova, Lilia E.; Sorokina, Alyona V.; Шарипова, М. Р.; Bogomolnaya, Lydia M.

doi:10.1007/s12668-016-0264-3

Cited by 15 publications

(5 citation statements)

References 12 publications

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“…No reuse allowed without permission. Our study also reports S. plymuthica PW1 and S. liquefaciens PW71 from textile dye effluent produce catecholate siderophores (Table 1), which is common among stress-tolerant bacterial genera [51]. Different Serratia species thrive well in contaminated environments such as textile dye effluent and petroleum [52].…”

Section: Assessment Of Siderophore Production In Bacteria and Dye Degradation In Algaesupporting

confidence: 63%

Iron-dependent mutualism between Chlorella sorokiniana and Ralstonia pickettii forms the basis for a sustainable bioremediation system

Rawat

Sharma

Poria

et al. 2021

Preprint

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Mutualism between microalgae and bacteria is ubiquitous, but remains underexplored as a basis for biodegradation of anthropogenic pollutants. In industrial systems, poor iron uptake by microalgae limits growth, bioprocessing efficacy, and bioremediation potential. Iron supplementation is costly and ineffective because iron remains insoluble in aqueous medium and biologically unavailable. In aquatic environments, microalgae develop an association with bacteria that solubilize iron by production of siderophore, which increases the bioavailability of iron as a public good. Algae, in exchange, provides dissolved organic matter to bacteria to sustain such interkingdom associations. Therefore, using a case study of azo dye degradation, we combine environmental isolations and synthetic ecology as a workflow, establishing a microbial community to degrade industrially relevant Acid Black 1 dye. We create a mutualism between previously non-associated chlorophyte alga Chlorella sorokiniana and siderophore-producing bacterium Ralstonia pickettii, based on the eco-evolutionary principle of exchange of iron and carbon. This siderophore-mediated increased iron bioavailability increases reductive iron uptake, growth rate, and azoreductase-mediated dye degradation of microalga. In exchange, C. sorokiniana produces galactose, glucose, and mannose as major extracellular monosaccharides, supporting bacterial growth. We propose a mechanism whereby extracellular ferrireductase is crucial for azoreductase-mediated dye degradation in microalgae. Our work demonstrates that bioavailability of iron, which is often overlooked in industrial bio-designs, governs microalgal growth and enzymatic processes. Our results suggest that algal-bacterial consortia based on the active association are a self-sustainable mechanism to overcome existing challenges of micronutrient availability in bioremediation systems and their industrial translation.

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Section: Assessment Of Siderophore Production In Bacteria and Dye Degradation In Algaesupporting

confidence: 63%

Iron-dependent mutualism between Chlorella sorokiniana and Ralstonia pickettii forms the basis for a sustainable bioremediation system

Rawat

Sharma

Poria

et al. 2021

Preprint

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“…More recently, MacAB was shown to be involved in survival of S. Typhimurium during oxidative stress through secretion of linearized siderophore enterobactin ( 27 , 28 ). Interestingly, production of S. marcescens siderophores is affected by deletion of the MacAB efflux pump ( 72 ). Our results further demonstrate that the role of the MacAB efflux pump in protection from oxidative stress is conserved across different bacterial species.…”

Section: Discussionmentioning

confidence: 99%

The ABC-Type Efflux Pump MacAB Is Involved in Protection of Serratia marcescens against Aminoglycoside Antibiotics, Polymyxins, and Oxidative Stress

Shirshikova

Sierra-Bakhshi

Kamaletdinova

et al. 2021

mSphere

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Serratia marcescens is an emerging pathogen with increasing clinical importance due to its intrinsic resistance to several classes of antibiotics. The chromosomally encoded drug efflux pumps contribute to antibiotic resistance and represent a major challenge for the treatment of bacterial infections. The ABC-type efflux pump MacAB was previously linked to macrolide resistance in Escherichia coli and Salmonella enterica serovar Typhimurium. The role of the MacAB homolog in antibiotic resistance of S. marcescens is currently unknown. We found that an S. marcescens mutant lacking the MacAB pump did not show increased sensitivity to the macrolide antibiotic erythromycin but was significantly more sensitive to aminoglycoside antibiotics and polymyxins. We also showed that, in addition to its role in drug efflux, the MacAB efflux pump is required for swimming motility and biofilm formation. We propose that the motility defect of the ΔmacAB mutant is due, at least in part, to the loss of functional flagella on the bacterial surface. Furthermore, we found that the promoter of the MacAB efflux pump was active during the initial hours of growth in laboratory medium and that its activity was further elevated in the presence of hydrogen peroxide. Finally, we demonstrate a complete loss of ΔmacAB mutant viability in the presence of peroxide, which is fully restored by complementation. Thus, the S. marcescens MacAB efflux pump is essential for survival during oxidative stress and is involved in protection from polymyxins and aminoglycoside antibiotics. IMPORTANCE The opportunistic pathogen Serratia marcescens can cause urinary tract infections, respiratory infections, meningitis, and sepsis in immunocompromised individuals. These infections are challenging to treat due to the intrinsic resistance of S. marcescens to an extensive array of antibiotics. Efflux pumps play a crucial role in protection of bacteria from antimicrobials. The MacAB efflux pump, previously linked to efflux of macrolides in Escherichia coli and protection from oxidative stress in Salmonella enterica serovar Typhimurium, is not characterized in S. marcescens. We show the role of the MacAB efflux pump in S. marcescens protection from aminoglycoside antibiotics and polymyxins, modulation of bacterial motility, and biofilm formation, and we illustrate the essential role for this pump in bacterial survival during oxidative stress. Our findings make the MacAB efflux pump an attractive target for inhibition to gain control over S. marcescens infections.

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“…For example, in P. aeruginosa the PvdRT-OpmQ tripartite system is involved in the secretion and recycling of the multifunctional siderophore pyoverdine (92)(93)(94). In Serratia marcescens, the ABC-type tripartite complex MacAB-HasF has been implicated in enterobactin secretion (95). A recent study in S. Typhimurium has shown that MacAB extrudes linearized siderophore metabolites in an iron-independent manner, to protect the cell from oxidative stress (85).…”

Section: Proposed Physiological Functions: Detoxification Of Endogenously Synthesized Substrates and Ph Homeostasismentioning

confidence: 99%

The Evolutionary Conservation of Escherichia coli Drug Efflux Pumps Supports Physiological Functions

Teelucksingh¹,

Thompson²,

Cox³

2020

J Bacteriol

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Bacteria harness an impressive repertoire of resistance mechanisms to evade the inhibitory action of antibiotics. One such mechanism involves efflux pump-mediated extrusion of drugs from the bacterial cell, which significantly contributes to multidrug resistance. Intriguingly, most drug efflux pumps are chromosomally encoded components of the intrinsic antibiotic resistome. In addition, in terms of xenobiotic detoxification, bacterial efflux systems often exhibit significant levels of functional redundancy. Efflux pumps are also considered to be highly conserved; however, the extent of conservation in many bacterial species has not been reported and the majority of genes that encode efflux pumps appear to be dispensable for growth. These observations, in combination with an increasing body of experimental evidence, imply alternative roles in bacterial physiology. Indeed, the ability of efflux pumps to facilitate antibiotic resistance could be a fortuitous by-product of ancient physiological functions. Using Escherichia coli as a model organism, here we evaluated the evolutionary conservation of drug efflux pumps and we provide phylogenetic analysis of the major efflux families. We show the E. coli drug efflux system has remained relatively stable and the majority (∼80%) of pumps are present in the core proteome. This analysis further supports the importance of drug efflux pumps in E. coli physiology. In this review, we also provide an update on the role of drug efflux pumps in the detoxification of endogenously synthesized substrates and pH homeostasis. Overall, gaining insight into drug efflux pump conservation, common evolutionary ancestors and physiological functions, could enable strategies to combat these intrinsic and ancient elements.

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Production of Siderophores by Serratia marcescens and the Role of MacAB Efflux Pump in Siderophores Secretion

Cited by 15 publications

References 12 publications

Iron-dependent mutualism between Chlorella sorokiniana and Ralstonia pickettii forms the basis for a sustainable bioremediation system

Iron-dependent mutualism between Chlorella sorokiniana and Ralstonia pickettii forms the basis for a sustainable bioremediation system

The ABC-Type Efflux Pump MacAB Is Involved in Protection of Serratia marcescens against Aminoglycoside Antibiotics, Polymyxins, and Oxidative Stress

The Evolutionary Conservation of Escherichia coli Drug Efflux Pumps Supports Physiological Functions

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