Production of <scp>AI</scp>‐2 is mediated by the S‐ribosylhomocystein lyase gene lux<scp>S</scp> in Bacteroides fragilis and Bacteroides vulgatus

Peixoto, Rafael José Marques; Miranda, Karla Rodrigues; Ferreira, Eliane de Oliveira; Paula, Geraldo Renato de; Rocha, Edson R.; Lobo, Leandro Araújo; Domingues, Regina Maria Cavalcanti Pilotto

doi:10.1002/jobm.201300311

Cited by 8 publications

(3 citation statements)

References 28 publications

(58 reference statements)

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“…and Prevotella spp. (shown in Table 1) were presumed to be xylanolytic because of the presence of genes encoding xynalases in their genome (Antunes et al 2005;Lukas et al 2008;Peixoto et al 2013), and strains of Bacteroides spp. and Prevotella spp.…”

Section: Results and Discussion Detection Of Luxs Gene Sequencesmentioning

confidence: 99%

Mining ofluxSgenes from rumen microbial consortia by metagenomic and metatranscriptomic approaches

Ghali

Shinkai

Mitsumori

2015

Animal Science Journal

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Although rumen bacterial communities vary depending on many factors such as diet, age and physiological conditions, a core microbiota exists within the rumen. In many natural environments, some bacteria use a quorum-sensing (QS) system to regulate their physiological activities. However, very limited information is available about QS systems in rumen. To investigate the autoinducer 2 (AI-2)-mediated QS system in rumen, we detected genes (luxS) encoding the AI-2 synthase (LuxS), from three datasets embedded in metagenomics RAST server (MG-RAST) and from a metatranscriptome dataset. We collected 135 luxS genes from the metagenomic datasets, which were presumed to originate from Bacteroidetes, Firmicutes, Fusobacteria and Actinobacteria, and 34 luxS genes from the metatranscriptome dataset, which probably originated from Bacteroidetes, Firmicutes and Spirochaetes. Because the essential amino acids for LuxS activity were conserved in the LuxS homologues predicted from luxS gene sequences from both datasets, the LuxS homologues probably function in the rumen. Since the largest number of sequences of luxS genes were collected from the genera Prevotella, Ruminococcus and Eubacterium, which include many fibrolytic bacteria and constituent members of biofilm on feed particles, an AI-2-mediated QS system is likely involved in biofilm formation and fibrolytic activity in the rumen.

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Section: Results and Discussion Detection Of Luxs Gene Sequencesmentioning

confidence: 99%

Mining ofluxSgenes from rumen microbial consortia by metagenomic and metatranscriptomic approaches

Ghali

Shinkai

Mitsumori

2015

Animal Science Journal

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“…Interestingly, E. coli is able to intercept extracellular AI-2 produced by other bacterial species in vitro (Peixoto et al, 2014). As the microbiota produces AI-2 like molecules (Mitsumori et al, 2003), it is possible that Enterobacteriaceae can utilize interspecies signaling in the gut.…”

Section: Multidirectional Signaling In the Gut And Effect On S Typhimentioning

confidence: 99%

Salmonella Typhimurium and Multidirectional Communication in the Gut

et al. 2016

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The mammalian digestive tract is home to trillions of microbes, including bacteria, archaea, protozoa, fungi, and viruses. In monogastric mammals the stomach and small intestine harbor diverse bacterial populations but are typically less populated than the colon. The gut bacterial community (microbiota hereafter) varies widely among different host species and individuals within a species. It is influenced by season of the year, age of the host, stress and disease. Ideally, the host and microbiota benefit each other. The host provides nutrients to the microbiota and the microbiota assists the host with digestion and nutrient metabolism. The resident microbiota competes with pathogens for space and nutrients and, through this competition, protects the host in a phenomenon called colonization resistance. The microbiota participates in development of the host immune system, particularly regulation of autoimmunity and mucosal immune response. The microbiota also shapes gut–brain communication and host responses to stress; and, indeed, the microbiota is a newly recognized endocrine organ within mammalian hosts. Salmonella enterica serovar Typhimurium (S. Typhimurium hereafter) is a food-borne pathogen which adapts to and alters the gastrointestinal (GI) environment. In the GI tract, S. Typhimurium competes with the microbiota for nutrients and overcomes colonization resistance to establish infection. To do this, S. Typhimurium uses multiple defense mechanisms to resist environmental stressors, like the acidic pH of the stomach, and virulence mechanisms which allow it to invade the intestinal epithelium and disseminate throughout the host. To coordinate gene expression and disrupt signaling within the microbiota and between host and microbiota, S. Typhimurium employs its own chemical signaling and may regulate host hormone metabolism. This review will discuss the multidirectional interaction between S. Typhimurium, host and microbiota as well as mechanisms that allow S. Typhimurium to succeed in the gut.

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“…It has recently been demonstrated that Bacteroides species can produce AI-2 like molecules [ 181 ], and heterologous expression of Bacteroides luxS orthologues can complement AI-2 production in E. coli [ 182 ]. As members of the intestinal microbiota produce AI-2, it is not inconceivable that members of these dense communities exploit LuxS-based signaling to modulate gene expression and community phenotypes.…”

Section: Multidirectional Chemical Signaling Within the Gastrointementioning

confidence: 99%

The Dynamic Interactions between Salmonella and the Microbiota, within the Challenging Niche of the Gastrointestinal Tract

Khan

2014

International Scholarly Research Notices

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Understanding how Salmonella species establish successful infections remains a foremost research priority. This gastrointestinal pathogen not only faces the hostile defenses of the host's immune system, but also faces fierce competition from the large and diverse community of microbiota for space and nutrients. Salmonella have solved these challenges ingeniously. To jump-start growth, Salmonella steal hydrogen produced by the gastrointestinal microbiota. Type 3 effector proteins are subsequently secreted by Salmonella to trigger potent inflammatory responses, which generate the alternative terminal electron acceptors tetrathionate and nitrate. Salmonella exclusively utilize these electron acceptors for anaerobic respiration, permitting metabolic access to abundant substrates such as ethanolamine to power growth blooms. Chemotaxis and flagella-mediated motility enable the identification of nutritionally beneficial niches. The resulting growth blooms also promote horizontal gene transfer amongst the resident microbes. Within the gastrointestinal tract there are opportunities for chemical signaling between host cells, the microbiota, and Salmonella. Host produced catecholamines and bacterial autoinducers form components of this chemical dialogue leading to dynamic interactions. Thus, Salmonella have developed remarkable strategies to initially shield against host defenses and to transiently compete against the intestinal microbiota leading to successful infections. However, the immunocompetent host is subsequently able to reestablish control and clear the infection.

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Production of AI‐2 is mediated by the S‐ribosylhomocystein lyase gene luxS in Bacteroides fragilis and Bacteroides vulgatus

Cited by 8 publications

References 28 publications

Mining ofluxSgenes from rumen microbial consortia by metagenomic and metatranscriptomic approaches

Mining ofluxSgenes from rumen microbial consortia by metagenomic and metatranscriptomic approaches

Salmonella Typhimurium and Multidirectional Communication in the Gut

The Dynamic Interactions between Salmonella and the Microbiota, within the Challenging Niche of the Gastrointestinal Tract

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