Production of salmon calcitonin I in Oncorhynchus gorbuscha by alternative polyadenylation of two RNA species

Martial, Kelly; Maubras, L.; Taboulet, J.; Jullienne, A.; Milhaud, G; Moukhtar, M.S.; Cressent, M.

doi:10.1016/0378-1119(94)90161-9

Cited by 5 publications

(4 citation statements)

References 18 publications

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“…Intriguingly, their patterns of tissue expression were remarkably different: sCT-IV mRNA was detected in the testis, whereas sCT-V mRNA occurred in most tissues examined. Expression of CT mRNA had already been reported in the gills of pink salmon and rainbow trout (Martial et al 1994, Sasayama 1999), but our RT-PCR analysis revealed that the CT mRNA expressed in this tissue is only sCT-I. In addition, in situ hybridization histochemistry showed expression of sCT-IV gene in the Leydig cells of trout testis.…”

Section: Discussionsupporting

confidence: 55%

Identification and tissue distribution of mRNAs encoding salmon-type calcitonins-IV and -V in the rainbow trout

Hidaka

Suzuki

2004

Journal of Molecular Endocrinology

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Four types of calcitonin are produced in salmonid fish, although their functional diversity is almost unknown. To explore the significance of these isoforms, we have characterized salmon-type calcitonin (sCT) mRNAs in the rainbow trout (Oncorhynchus mykiss), and examined their tissue distribution. In addition to the previously isolated sCT-I cDNAs, two new forms of sCT cDNA were cloned from the ultimobranchial gland, and one of them (sCT-IV cDNA) was predicted to encode an N-terminal peptide of 80 amino acid residues, a putative cleavage site Lys-Arg, sCT-IV, a cleavage and amidation sequence Gly-Lys-Lys-Arg, and a C-terminal peptide of 18 amino acids. The sCT-IV precursor was 78% identical with the rainbow trout sCT-I precursors. The other cloned cDNA encoded a precursor for a novel CT, sCT-V. The sCT-V peptide was different from sCT-IV by only one amino acid residue: Val at position 8 in the latter was replaced by Met. The sCT-V precursor had 80 and 90% identity with the sCT-I and -IV precursors respectively. No cDNA clones were obtained for sCTs-II or -III.Tissue distribution of sCT-I, -IV and -V mRNAs was examined by RT-PCR and specific cleavage with restriction enzymes. An amplified fragment from sCT-I mRNA was detected not only in the ultimobranchial gland, but also in the gills, testis and ovary. RT-PCR analysis coupled to restriction digestion further revealed that sCT-IV mRNA was expressed in both the testis and the ultimobranchial gland. The expression sites of sCT-IV mRNA were localized to the Leydig cells of the testis and to the parenchymal cells of the ultimobranchial gland, by in situ hybridization histochemistry. Although the amino acid sequence of sCT-V peptide was nearly the same as that of sCT-IV, the sCT-V gene showed a much wider pattern of expression: the band amplified by RT-PCR was detected in all the tissues examined except the kidney, gills and blood cells. The sCT-V mRNA was shown to be localized in the parenchymal cells of the ultimobranchial gland, but not in other tissues at the cellular level, suggesting very low expression of sCT-V mRNA in those tissues. Our results show different patterns of tissue expression of three types of sCT genes in the rainbow trout, suggesting that sCTs-I, -IV and -V might differ in their local actions.

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Section: Discussionsupporting

confidence: 55%

Identification and tissue distribution of mRNAs encoding salmon-type calcitonins-IV and -V in the rainbow trout

Hidaka

Suzuki

2004

Journal of Molecular Endocrinology

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“…After separated on 16.5% T/3% C SDS-PAGE, a prominent band of 3.5 kDa was immuno-detected in both lanes, confirming the specificity of this antibody and the production of sCT-I peptide in the ultimobranchial gland. species by alternative polyadenylation (Martial et al, 1994). The initiation site for translation was predicted to be ATG at positions 1 to 3 in each cDNA (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…regulation of the sperm mobility and capacitation (Gnessi et al, 1984;Fraser et al, 2001), and of the embryonic implantation and development (Ding et al, 1994;Wang et al, 1998). As for fish, accumulating evidence also indicates that CT may be synthesized in various tissues including the ultimobranchial gland, the major production site of fish CT (Martial et al, 1994;Sasayama 1999;Clark et al, 2002). Although its physiological significance is controversial, it is suggested that serum fish CT might protect scales from excessive resorption in the goldfish ( Carassius auratus ) and nibbler fish ( Girella punctata ) (Suzuki et al, 2000a), and be involved in the reproductive physiology of rainbow trout ( Oncorhynchus mykiss ) (Bjornsson et al, 1986).…”

Section: Introductionmentioning

confidence: 99%

Shift of Chloride Cell Distribution during Early Life Stages in Seawater-Adapted Killifish, Fundulus heteroclitus

et al. 2000

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“…Existence of multiple isoforms of CT in salmonids may be explained by the genomic tetraploidization event they underwent, leading to the presence of extensive gene duplication, and also by the persistence during evolution of a productive CT sequence. In addition to the presence of such multiple isoforms in salmon, we recently reported that different mRNA pathways may be used to produce the sCT I molecule (25). These differences between human and salmon species are in favor of an important physiological role for the peptide in salmon species, a role that would have disappeared during evolution.…”

Section: Discussionmentioning

confidence: 99%

Identification of a new calcitonin gene in the salmon Oncorhynchus gorbuscha.

Jansz¹,

Martial²,

Zandberg³

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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Three isoforms of calcitonin (CT) exist in salmonids. Isohormones I and II are expressed in the pink salmon Oncorhynchus gorbuscha. We report here the existence in this species ofa CT gene and ofits transcripts, which encode for a fourth isohormone, the salmon CT (sCT) IV. This new CT gene was identified by PCR from genomic DNA and by sequencing the amplified DNA. The expression of this CT gene was established in ultimobranchial body and brain, by reverse transcription-PCR, hybridization and sequencing. The sCT IV gene, like the sCT I gene, is a complex transcription unit, containing exons encoding for a CT as a calcitonin generelated peptide (CGRP) molecule. The predicted peptide, sCT IV, has a greater homology with the eel CT and the sCT II than with the sCT I. Alignment of the sCT IV with other fish and chicken CT showed amino acid modifications in similar positions as those found during evolution. The predicted salmon CGRP IV peptide is highly homologous to the known CGRP molecules in other species, confirming the high conservation of the molecule during evolution. This identification of a new salmon CT gene is interesting both for the therapeutic potential represented by the new molecules encoded by this gene and for phylogenetic studies.

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Production of salmon calcitonin I in Oncorhynchus gorbuscha by alternative polyadenylation of two RNA species

Cited by 5 publications

References 18 publications

Identification and tissue distribution of mRNAs encoding salmon-type calcitonins-IV and -V in the rainbow trout

Identification and tissue distribution of mRNAs encoding salmon-type calcitonins-IV and -V in the rainbow trout

Shift of Chloride Cell Distribution during Early Life Stages in Seawater-Adapted Killifish, Fundulus heteroclitus

Identification of a new calcitonin gene in the salmon Oncorhynchus gorbuscha.

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