Production of S-(+)-ibuprofen with high optical purity from a nitrile compound by cells immobilized on cellulose porous beads

Takagi, Mutsumi; Shirokaze, J.; Oishi, Kazuhiko; Otsubo, Kazumasa; Yamamoto, Keizou; Yoshida, Nobuyuki; Fujimatsu, Isao

doi:10.1016/0922-338x(94)90264-x

Cited by 11 publications

(3 citation statements)

References 9 publications

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“…Ltd., which has a pore diameter of less than 100 m, and whose surface can be modified with DEAE group ions to have a positive charge (Takagi et al, 1994). Almost all the artificial liver models referred to above utilized porcine or rat hepatocyte cells, because human and animal hepatocyte cells cannot grow in a culture while retaining their differentiated properties.…”

Section: Introductionmentioning

confidence: 99%

Untitled

1997

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Selection of a cell line suitable for a hybrid artificial liver model employing cellulose porous beads (CPBs) was investigated. Hep G2 cells grown in a culture dish exhibited appreciably higher ureogenesis and gluconeogenesis activities than those grown in CPBs. SEM observation of CPBs revealed marked difference in the distribution of attached cells from one bead to another, and showed that almost all the cell-bearing micropores were completely packed with cells.With the aim of selecting a cell line not prone to excessive aggregation and which grows moderately so as not to fill up the micropores, cells of 6 cell lines, HLE, HLF, Hep 3B, PLC/PRF/5, Huh 7 and Hep G2, were cultivated in dishes. Hep G2, HLE, and HLF increased to 5 × 10(5) cells/cm(2), whereas PLC/PRF/5 grew only to 5 × 10(4), and Hep 3B and Huh 7 up to 2 × 10(4) cells/cm(2). The specific activities of ureogenesis and gluconeogenesis of Huh 7 were the highest among the lines tested - 42- and 7-fold those of Hep G2, respectively. When the 6 cell lines were grown in a submerged culture with 0.6 g/l of CPBs, Huh 7 had the lowest cell concentration of 0.54 × 10(6) cells/ml, and the highest activities of ammonia consumption and urea and glucose production (1.38 μ mol NH(3), 99 nmol urea, and 14.5 nmol glucose/10(6)cells/h). Consequently, Huh 7 is considered to be a suitable cell line for use in the development of an artificial liver model employing porous beads.

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Section: Introductionmentioning

confidence: 99%

Untitled

1997

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“…Most of the bioconversions of nitriles have been carried out using free cells and little information is available on the use of immobilized microbes for transformation of nitriles (Dias et al, 2000b ;Takagi et al, 1994 ;Nawaz et al, 1993 ). Among the various methods available for immobilization of cells (Chibata, 1978 ), yeasts have been immobilized by entrapment in alginate, κ-carageenan and citric pectin (Dias et al, 2000b ).…”

Section: Biotransformation Of Nitriles and Amides With Immobilized Cementioning

confidence: 98%

Nitrile Metabolizing Yeasts

Bhalla

Sharma

2009

Yeast Biotechnology: Diversity and Applications

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“…Despite the demonstrated importance of this technology there are relatively few other reports of immobilised biocatalysts able to hydrolyse nitriles (Crosby et al, 1994;De Raadt et al,1 993;Takagi et al, 1994).…”

Section: Introductionmentioning

confidence: 99%