Production of rhamnolipids with different proportions of mono-rhamnolipids using crude glycerol and a comparison of their application potential for oil recovery from oily sludge

Zhao, Feng; Jiang, Hao; Sun, Huichun; Liu, Chang; Han, Shun

doi:10.1039/c8ra09351b

Cited by 38 publications

(12 citation statements)

References 45 publications

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“…Although the use of rhamnolipids in enhanced oil recovery has been tested before, until recently, the production of rhamnolipid variants and their efficiency in MEOR have become a research hotspot [16,17,18]. In this study, the rhamnolipid-producing strain P. aeruginosa YM4 was selected for its capacity to efficiently produce Rha-Rha-C 10 -C 10 as the major component with soybean oil and glycerol as carbon source.…”

Section: Introductionmentioning

confidence: 99%

High-Yield Di-Rhamnolipid Production by Pseudomonas aeruginosa YM4 and its Potential Application in MEOR

Zhang

Lin

et al. 2019

Molecules

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Rhamnolipids are a mixture of the homologs species due to variations in the rhamnose units and β-hydroxy fatty acid moieties, mainly including Rha-C10-C10, Rha-Rha-C10-C10, and Rha-C10. In this study, strain P. aeruginosa YM4 was selected for its capacity to efficiently produce di-rhamnolipid (Rha-Rha-C10-C10) as the predominant component with soybean oil and glycerol as carbon source, accounting for 64.8% and 85.7% of total products, respectively. The critical micelle concentration (CMC) of rhamnolipid products varies with the content of di-rhamnolipid, whereby lower CMC values corresponding to higher di-rhamnolipid contents. The rhamnolipids containing 85.7% di-rhamnolipid had the lowest CMC value of 50 mg/L. Accordingly the viscosity-reducing efficiency and oil-washing efficiency of rhamnolipids increased with higher di-rhamnolipid component. At a concentration of 500 mg/L, the rhamnolipids containing 85.7% di-rhamnolipid worked best and showed 82.5% oil-washing efficiency, which offered great promise for applications in enhanced oil recovery. The results showed the variation of structure and composition of rhamnolipids had a significant effect on their application.

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Section: Introductionmentioning

confidence: 99%

High-Yield Di-Rhamnolipid Production by Pseudomonas aeruginosa YM4 and its Potential Application in MEOR

Zhang

Lin

et al. 2019

Molecules

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“…The mass spectrometer was in the negative ESI mode and the scanning mass range was from 200 to 900 m/z (mass to charge ratio). RL congeners were identified based on m/z and their relative proportions were calculated using the obtained area for each congener of RL by HPLC–MS (Haba et al 2014 ; Zhao et al 2019 ).…”

Section: Methodsmentioning

confidence: 99%

“…Accordingly, they may be used in various industries like pharmaceuticals, cosmetics, food and agriculture. Moreover, they are also used in enhanced oil recovery (EOR) and bioremediation of heavy metals and hydrocarbon pollutants (Zhao et al 2019 ). The use of chemical surfactants for enhancing oil recovery and biodegradation is unfavorable as they are toxic, expensive and may negatively impact the environment.…”

Section: Introductionmentioning

confidence: 99%

Structural and Physicochemical Characterization of Rhamnolipids produced by Pseudomonas aeruginosa P6

El-Housseiny¹,

Aboshanab²,

Aboulwafa

et al. 2020

AMB Expr

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Rhamnolipids are important biosurfactants for application in bioremediation, enhanced oil recovery, pharmaceutical, and detergent industry. In this study, rhamnolipids extracted from P. aeruginosa P6 were characterized to determine their potential fields of application. Thin-layer chromatographic analysis of the produced rhamnolipids indicated the production of two homologues: mono- and di-rhamnolipids, whose structures were verified by 1H and 13C nuclear magnetic resonance spectroscopy. Additionally, high performance liquid chromatography-mass spectrometry identified seven different rhamnolipid congeners, of which a significantly high proportion was di-rhamnolipids reaching 80.16%. Rha-Rha-C10-C10 was confirmed as the principal compound of the rhamnolipid mixture (24.30%). The rhamnolipids were capable of lowering surface tension of water to 36 mN/m at a critical micelle concentration of 0.2 g/L, and exhibited a great emulsifying activity (E24 = 63%). In addition, they showed excellent stability at pH ranges 4–8, NaCl concentrations up to 9% (w/v) and temperatures ranging from 20 to 100 °C and even after autoclaving. These results suggest that rhamnolipids, produced by P. aeruginosa P6 using the cheap substrate glycerol, are propitious for biotechnology use in extreme and complex environments, like oil reservoirs and hydrocarbon contaminated soil. Moreover, P. aeruginosa P6 may be considered, in its wild type form, as a promising industrial producer of di-RLs, which have superior characteristics for potential applications and offer outstanding commercial benefits.

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“…Results showed that using crude glycerol for anaerobic production of rhamnolipids by P. aeruginosa is feasible. Previous studies reported aerobic production of rhamnolipids by P. aeruginosa using crude glycerol [39,40]. Using glycerol or crude glycerol, the rhamnolipids yields from P. aeruginosa were both lower under anaerobic conditions.…”

Section: Ftir Spectra Analysis Of Anaerobically Produced Rhamnolipids Using Glycerol and Nitratementioning

confidence: 95%

Glycerol promoted the anaerobic production of rhamnolipids by different Pseudomonas aeruginosa strains

Zhao

Zheng

Cui

2021

Preprint

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Background: Rhamnolipids is the most widely studied and applied biosurfactants. The anaerobic biosynthesis of rhamnolipids has important research and practical significance, such as meeting the in situ production of biosurfactant in anoxic environments and the foamless fermentation of biosurfactants. A few studies have reported the anaerobic biosynthesis of rhamnolipids from rare Pseudomonas aeruginosa strains. What did promote the anaerobic biosynthesis of rhamnolipids, the specificity of the rare strains or the effect of specific substrates? Here, anaerobic production of rhamnolipids by different P. aeruginosa strains was investigated using diverse substrates. The anaerobic biosynthesis mechanism of rhamnolipids were also discussed from the substrate point of view.Results: All P. aeruginosa strains anaerobically grew well using the tested substrates. But all P. aeruginosa strains anaerobically produced rhamnolipids only using substrates containing glycerol and nitrate. Fourier transform infrared (FTIR) spectra analysis confirmed the anaerobic production of rhamnolipids from all P. aeruginosa strains. All the anaerobically produced rhamnolipids decreased air-water surface tension from 72.6 mN/m to below 29.0 mN/m and emulsified crude oil with EI24 above 65%. Using crude glycerol as low-cost substrate, all P. aeruginosa strains can anaerobically grow and produce rhamnolipids to reduce the culture surface tension below 35 mN/m. The glycerol metabolic intermediate, 1, 2-propylene glycol, can also achieve the anaerobic production of rhamnolipids by all P. aeruginosa strains.Conclusions: Not the specificity of the rare P. aeruginosa strains but the effect of specific substrates promote the anaerobic biosynthesis of rhamnolipids by P. aeruginosa. Glycerol and nitrate are the excellent substrates for anaerobic production of rhamnolipids from all P. aeruginosa strains. Results indicated that glycerol metabolism involveed the anaerobic biosynthesis of rhamnolipids in P. aeruginosa. Results also showed the feasibility of using crude glycerol as low cost substrate to anaerobically biosynthesize rhamnolipids by P. aeruginosa.

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Production of rhamnolipids with different proportions of mono-rhamnolipids using crude glycerol and a comparison of their application potential for oil recovery from oily sludge

Cited by 38 publications

References 45 publications

High-Yield Di-Rhamnolipid Production by Pseudomonas aeruginosa YM4 and its Potential Application in MEOR

High-Yield Di-Rhamnolipid Production by Pseudomonas aeruginosa YM4 and its Potential Application in MEOR

Structural and Physicochemical Characterization of Rhamnolipids produced by Pseudomonas aeruginosa P6

Glycerol promoted the anaerobic production of rhamnolipids by different Pseudomonas aeruginosa strains

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