Production of Remazol Brilliant Blue R decolourising oxygenase from the culture filtrate of Funalia trogii ATCC 200800

Deveci, Tayfun; Ünyayar, Ali; Mazmancı, Mehmet Ali

doi:10.1016/j.molcatb.2004.03.002

Cited by 98 publications

(44 citation statements)

References 53 publications

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“…The use of dyes as model compounds in pollutant biodegradation studies offers a series of advantages compared to conventional substrates: dyes are stable, soluble, possess high molar extinction coefficients and low toxicity, and can be applied in simple, rapid and quantitative spectrophotometric assays. RBBR has been widely used since, as an anthracene derivative, this compound represents an important group of organopollutants (Novotny et al, 2004;Deveci et al, 2004). In the present study, the initial pH of the culture medium influenced the in vitro decolorization of RBBR by the fungi studied.…”

Section: Resultsmentioning

confidence: 77%

Influence of pH on the growth, laccase activity and RBBR decolorization by tropical basidiomycetes

Neto

Matheus

Machado

2009

Braz. arch. biol. technol.

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The basidiomycete fungi Lentinus crinitus and Psilocybe castanella are being evaluated in a bioremediation process of soils contaminated with organochlorine industrial residues in the Baixada Santista, São Paulo. The aim of the present study was to determine the influence of pH on the fungal growth, in vitro decolorization of anthraquinonic dye Remazol Brilliant Blue R (RBBR) and laccase activity. The pH of the culture medium influenced the growth of L. crinitus and P. castanella, which presented less growth at pH 5.9 and pH 2.7, respectively. The fungi were able to modify the pH of the culture medium, adjusting it to the optimum pH for growth which was close to 4.5. Decolorization of the RBBR was maximal at a pH of 2.5 to 3.5. Higher laccase activity was observed at pH 3.5 and pH 4.5 for L. crinitus and P. castanella, respectively. pH was found to be an important parameter for both the growth of these fungi and the enzymatic system involved in RBBR decolorization.

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Section: Resultsmentioning

confidence: 77%

Influence of pH on the growth, laccase activity and RBBR decolorization by tropical basidiomycetes

Neto

Matheus

Machado

2009

Braz. arch. biol. technol.

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“…This finding was supported by the significant inhibition of RBBR decolorization in the presence of typical oxidoreductase inhibitors such as sodium azide, ferrous sulfate and EDTA. Inhibition of RBBR decolorization by these agents has been reported for both crude extract and purified laccase and MnP (4,14,22,27). Manganese sulfate did not alter RBBR decolorization by the P. ostreatus extract, as also observed by Vyas and Molitoris (27).…”

Section: Effect Of Inhibitory Substancesmentioning

confidence: 99%

“…Decolorization of RBBR by the extract of P. ostreatus grown for 25 days was monitored after preincubation of the extract with 50 µL of 1 mM of the inhibitor (H 2 O 2 , sodium azide, FeSO 4 or EDTA) for 5 min. HRP was used as reference.…”

Section: Effect Of Inhibitorsmentioning

confidence: 99%

“…2). Decolorization of RBBR by crude extracts and purified enzymes obtained from basidiomycetes grown on solid substrate has been shown to be maximal in the pH range from 4.0 to 5.0 (4,22,27).…”

Section: Optimal H2o2 Concentration and Ph Optimum For Rbbr Decolorizmentioning

confidence: 99%

“…It is the raw material in the production of polymeric dyes and, as a derivative of anthracene, represents an important member of toxic and recalcitrant organopollutants. The identity of enzymes involved in the degradation of RBBR is still not completely established, with evidence indicating the involvement of lignin peroxidase and laccases (4,17,24).…”

Section: Introductionmentioning

confidence: 99%

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Biodegradation of remazol brilliant blue R by ligninolytic enzymatic complex produced by Pleurotus ostreatus

Machado¹,

Matheus²

2006

Braz. J. Microbiol.

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Pleurotus ostreatus ("shimeji") is produced in Brazil on a commercial scale using various lignocellulosic residues. Efforts have been made to reuse the culture residue to obtain products of greater aggregate value such as enzymes or in processes of bioremediation. We evaluated the Remazol brilliant blue R (RBBR) degradation potential of extracts from solid substrate colonized by P. ostreatus and extracts from residue of the "shimeji" mushroom yield. Colonized substrates and residue were provided by Toyobo do Brasil Ltda. Extraction was performed with sodium acetate buffer (50 mM, pH 4.6). RBBR decolorization was monitored at 592 nm and peroxidase and laccase activities were measured by monitoring the oxidation of ABTS. Horseradish peroxidase was used as reference. The time of growth of P. ostreatus influenced RBBR degradation and peroxidase and laccase activities. Concentration of 1 mM H2O2 and pH 4.0 were the best for RBBR decolorization. Complete RBBR decolorization was obtained with the addition of only one aliquot of 50 µL of 1 mM H2O2. The stability of the extracts was higher when they were kept under refrigeration than when stored frozen. The potential application of the ligninolytic complex derived from P. ostreatus and mushroom residue for xenobiotic degradation was demonstrated.

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Synthesis of calix[6]arene based XAD‐4 material for the removal of reactive blue 19 from aqueous environments

Bhatti

Kamboh

Solangi

et al. 2013

J of Applied Polymer Sci

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This study describes the synthesis of calix[6]arene (C6) appended Amberlite XAD-4 resin and its application for the removal of anthraquinone-based reactive blue 19 (RB-19) dye from aqueous environments. The C6-resin 5 was characterized with various analytical techniques, including Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, and elemental analysis. Adsorption experiments were carried out to investigate the effects of the pH, adsorbent dosage, electrolyte, contact time, and temperature on the adsorption of RB-19 dye onto the C6-resin 5. From the results, we observed that the percentage adsorption of the RB-19 dye was highly dependent on the concentration of electrolyte and the pH of the solution. The maximum adsorption was achieved at pH 9. The thermal study demonstrated that the adsorption process was endothermic and spontaneous in nature. The isothermic study showed that the adsorption behavior could be better demonstrated by the Langmuir and Dubinin and Radushkevich isotherm model. From field studies, it has been concluded that C6-resin 5 is an effective adsorbent for the removal of RB-19 dye.

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Production of Remazol Brilliant Blue R decolourising oxygenase from the culture filtrate of Funalia trogii ATCC 200800

Cited by 98 publications

References 53 publications

Influence of pH on the growth, laccase activity and RBBR decolorization by tropical basidiomycetes

Influence of pH on the growth, laccase activity and RBBR decolorization by tropical basidiomycetes

Biodegradation of remazol brilliant blue R by ligninolytic enzymatic complex produced by Pleurotus ostreatus

Synthesis of calix[6]arene based XAD‐4 material for the removal of reactive blue 19 from aqueous environments

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