Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae

Ferrer, Marcela; Wehrendt, Diana Patricia; Bonilla, Mariana; Comini, Marcelo A.; Téllez-Iñón, Marı́a T.; Potenza, Mariana

doi:10.1007/978-1-4939-9148-8_8

Cited by 2 publications

(1 citation statement)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the heterologous expression in Escherichia coli (a prokaryote lacking the post-translational glycosylation system usually found in eukaryotes) probably altered the spatial conformation of our antigens, making them less specific for the Trypanozoon of interest. An alternative production system for recombinant antigens using a related trypanosomatid organism such as Leishmania tarentolae 21 , 22 could be promising, but its cost is prohibitive. Another recombinant antigen production system using the yeast Pichia pastoris that has been shown to be efficient in the production of trypanosomal antigens could provide a balance between the lack of post-translational modifications with the antigen production in E. coli and the cost of producing recombinant antigens in L. tarentolae 23 , 24 .…”

Section: Discussionmentioning

confidence: 99%

Development of a microsphere-based immunoassay for the serological diagnosis of equine trypanosomosis

Verney

Gautron

Lemans

et al. 2022

Sci Rep

View full text Add to dashboard Cite

Trypanozoon infections in equids are caused by three parasite species in the Trypanozoon subgenus: Trypanosoma equiperdum, T. brucei and T. evansi. They are respectively responsible for infectious diseases dourine, nagana and surra. Due to the threat that Trypanozoon infection represents for international horse trading, accurate diagnostic tests are crucial. Current tests suffer from poor sensitivity and specificity, due in the first case to the transient presence of parasites in the blood and in the second, to antigenic cross-reactivity among Trypanozoon subspecies. This study was designed to develop a microsphere‐based immunoassay for diagnosing equine trypanosomosis. We tested beads coated with eight Trypanosoma spp. recombinant antigens: enolase, GM6, PFR1, PFR2, ISG65, VSGat, RoTat1.2 and JN2118HU. Of these, GM6 was identified as the best candidate for the serological diagnosis of Trypanozoon infections in equids. Using a receiver operating characteristic (ROC) analysis on 349 equine sera, anti-GM6 antibodies were detected with an AUC value of 0.994 offering a sensitivity of 97.9% and a specificity of 96.0%. Our findings show that the GM6 antigen is a good target for diagnosing equine trypanosomosis using a microsphere‐based immunoassay. This promising assay could be a useful alternative to the official diagnostic tool for equine trypanosomosis.

show abstract