Production of recombinant single chain antibodies (scFv) in vegetatively reproductive Kalanchoe pinnata by in planta transformation

Jung, Yuchul; Rhee, Yong; Auh, Chung-Kyoon; Shim, Hyungbo; Choi, Jung-Jin; Kwon, Suk‐Tae; Yang, Joo-Sung; Kim, Donggiun; Kwon, Myung-Hee; Kim, Yong‐Sung; Lee, Sukchan

doi:10.1007/s00299-009-0758-3

Cited by 12 publications

(4 citation statements)

References 35 publications

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“…RNA was quantified by UV (230/260 nm), precipitated, resuspended in DEPC‐treated water at a concentration of 5 μg/μL and stored at −85 °C. cDNA was synthesized from 5 μg of RNA with Moloney Marine Leukemia Virus (MMLV) reverse transcriptase (Promega, Madison, WI, USA) using 200 ng oligo‐dT primers at 50 °C for 60 min (Jung et al 2009).…”

Section: Methodsmentioning

confidence: 99%

Expression Analysis of Proline Metabolism‐related Genes From Halophyte Arabis stelleri under Osmotic Stress Conditions

Jung

Park

Choi

et al. 2010

JIPB

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Arabis stelleri var. japonica evidenced stronger osmotic stress tolerance than Arabidopsis thaliana. Using an A. thaliana microarray chip, we determined changes in the expression of approximately 2 800 genes between A. stelleri plants treated with 0.2 M mannitol versus mock-treated plants. The most significant changes in the gene expression patterns were in genes defining cellular components or in genes associated with the endomembrane system, stimulus response, stress response, chemical stimulus response, and defense response. The expression patterns of three de novo proline biosynthesis enzymes were evaluated in A. stelleri var. japonica seedlings treated with 0.2 M mannitol, 0.2 M sorbitol, and 0.2 M NaCl. The expression of 1 -pyrroline-5-carboxylate synthetase was not affected by NaCl stress but was similarly induced by mannitol and sorbitol. The proline dehydrogenase gene, which is known to be repressed by dehydration stress and induced by free L-proline, was induced at an early stage by mannitol treatment, but the level of proline dehydrogenase was increased later by treatment with both mannitol and NaCl. The level of free L-proline accumulation increased progressively in response to treatments with mannitol, sorbitol, and NaCl. Mannitol induced L-proline accumulation more rapidly than NaCl or sorbitol. These findings demonstrate that the osmotic tolerance of the novel halophyte, Arabis stelleri, is associated with the accumulation of L-proline.

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Section: Methodsmentioning

confidence: 99%

Expression Analysis of Proline Metabolism‐related Genes From Halophyte Arabis stelleri under Osmotic Stress Conditions

Jung

Park

Choi

et al. 2010

JIPB

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“…In addition, this antibody is superior to accumulate at low levels and to induce no harmful effects on plant development in spite of its sequence-independent activity (Lee et al, 2013a). It has been reported that expression of 3D8 scFv confers resistance to DNA or RNA viruses in tobaccos and Chinese cabbages (Brassica rapa) (Lee et al, 2013a;Lee et al, 2013b;Jung et al, 2009). When CSVd was inoculated into transgenic chrysanthemums expressing the conventional 3D8 scFv or codon-optimized for chrysanthemum, no disease symptoms were observed in either case, and all transgenic lines showed no disease symptoms.…”

Section: -3-1-2 Nucleic Acid Antibodymentioning

confidence: 99%

Resistance and Tolerance to Viroid Infection

Nakada¹,

Li²,

Shimmen³

2022

Preprint

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Viroids are known the smallest plant pathogens, and although their genome sequences do not encode proteins, they can cause disease in economically important crops. In order to control viroid diseases and mitigate their damage, genetic resources used for breeding of the viroid-resistant crop have been searched, but the practical resistant trait has not been found in almost all viroid-crop combinations, as well as the tolerant trait. Due to the difficulty in exploiting naturally occurring resistance or tolerance, various effective strategies have been devised to control viroid diseases using non-transforming or transforming techniques. Meanwhile, extensive findings related to viroid resistance and tolerance may lead to confer resistance or tolerance to viroid infection by combining with the recently emerged new technologies (e.g., spray-induced gene silencing and genome-editing technologies), which are believed to be more environmentally viable and acceptable to the general public than previously reported approaches. In particular, some genome-modified crops produced by the latter technology are already on the market. In this review, we comprehensively summarize the current status about investigation of naturally occurring genetic traits for viroid resistance and tolerance, accumulating knowledge about host factors involved in viroid pathogenicity, and various basic technologies developed to try to possible viroid disease control strategies. Furthermore, we discuss prospects and challenges for the achievement of more effective, practical, and sustainable disease control of viroid.

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“…sp.) suckers [153], gemmae of umbrella liverwort (Marchantia polymorphya) [154], leaf notches of cathedral bells (Kalanchoe pinnata) [155], sugarcane (Saccharum spp.) setts [156], and bulbs of the Notocactus scopa and Hylocereus trigonus cacti [157].…”

Section: Propagule Transformationmentioning

confidence: 99%

A comprehensive review of in planta stable transformation strategies

Bélanger,

Copley,

Hoyos-Villegas

et al. 2024

Plant Methods

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Plant transformation remains a major bottleneck to the improvement of plant science, both on fundamental and practical levels. The recalcitrant nature of most commercial and minor crops to genetic transformation slows scientific progress for a large range of crops that are essential for food security on a global scale. Over the years, novel stable transformation strategies loosely grouped under the term “in planta” have been proposed and validated in a large number of model (e.g. Arabidopsis and rice), major (e.g. wheat and soybean) and minor (e.g. chickpea and lablab bean) species. The in planta approach is revolutionary as it is considered genotype-independent, technically simple (i.e. devoid of or with minimal tissue culture steps), affordable, and easy to implement in a broad range of experimental settings. In this article, we reviewed and categorized over 300 research articles, patents, theses, and videos demonstrating the applicability of different in planta transformation strategies in 105 different genera across 139 plant species. To support this review process, we propose a classification system for the in planta techniques based on five categories and a new nomenclature for more than 30 different in planta techniques. In complement to this, we clarified some grey areas regarding the in planta conceptual framework and provided insights regarding the past, current, and future scientific impacts of these techniques. To support the diffusion of this concept across the community, this review article will serve as an introductory point for an online compendium about in planta transformation strategies that will be available to all scientists. By expanding our knowledge about in planta transformation, we can find innovative approaches to unlock the full potential of plants, support the growth of scientific knowledge, and stimulate an equitable development of plant research in all countries and institutions.

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Production of recombinant single chain antibodies (scFv) in vegetatively reproductive Kalanchoe pinnata by in planta transformation

Cited by 12 publications

References 35 publications

Expression Analysis of Proline Metabolism‐related Genes From Halophyte Arabis stelleri under Osmotic Stress Conditions

Expression Analysis of Proline Metabolism‐related Genes From Halophyte Arabis stelleri under Osmotic Stress Conditions

Resistance and Tolerance to Viroid Infection

A comprehensive review of in planta stable transformation strategies

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