Production of recombinant proteins by sol–gel immobilized Escherichia coli

Encapsulation of cells within silica matrixes: Towards a new advance in the conception of living hybrid materials

“…In literature, some examples show the real beneficial effect of the encapsulation in the bioconversion of molecules, the de novo synthesis of metabolites [58] and even the production of recombinant proteins [67,68]. For instance, the entrapment of Methylomonas sp.…”

Two schemes for production of biosurfactant from Pseudomonas aeruginosa MR01: Applying residues from soybean oil industry and silica sol–gel immobilized cells

“…Moreover, pH variations, viscosity and rigidity increases due to gel formation, the aging process which causes shrinkage of the matrix and other changes occurring throughout the immobilization procedure may be among the stress factors harmful for cell activities [30]. However, further benefits of immobilized cells, particularly when entrapped in silica sol-gels [17,49]…”

“…Biosurfactant measurements taken after the completion of each batch, which required 7 days, showed that production efficiency was not only preserved during the three consecutive batches but also increased slightly (data shown in Fig 4). This may be attributed to the fact that the sol-gel technique leads to inorganic matrices possessing a high porosity [11,49]. The porous structure of silica gel [23,48] allows the diffusion of the nutritional compounds from the medium to the interior of the silica gels and the release of the extracellular metabolites and biosurfactants out of the gels [4].…”

Immobilization of Recombinant Bacterial Biosensors: a Simple Approach for the On-Site Detection of Phenolic Compounds

“…최근에 바이오센서의 민감도와 특이성을 향 상시키기 위해 조절 단백질의 유도자 결합 부위를 변형하거나 [8,15,23], 표면 발현 단백질 [13]과 주변세포질 결합 단백질들 [15] [1,4]. 고정화는 환경적 스트레스와 독성으로부터 세포를 보호한다 [5]. 물리적 고정화(포괄과 흡착)는 살아있는 세포가 필요할 경우, 화학적 고정화(cross-linking과 covalent binding)는 살아있는 세포가 중요하지 않을 경우 선택하는 방법이다 [13].…”