Production of recombinant Japanese eel gonadotropins by baculovirus in silkworm larvae

Experimental and computational study of inter- and intra- species specificity of gonadotropins for various gonadotropin receptors

“…The recombinant gonadotropin from less-evolved fish (eel and trout) were more efficient in eliciting estradiol secretion, while in mature ovarian fragments the DHP secretion was higher in response to recombinant LHs than to recombinant FSHs (Aizen et al, 2012). Moreover, biological activities of recombinant taLH and taFSH have been confirmed by steroid secretion from tilapia gonads (Kasuto and Levavi-Sivan, 2005;Aizen et al, 2007a;Levavi-Sivan et al, 2008), biological activities of eFSH and eLH by in vitro oocyte maturation and in vivo spermatogenesis (Kobayashi et al, 2010), and biological activities of trFSH and trLH by in vitro receptor activation, sex steroid production, and in vivo ovarian development and milt production (Ko et al, 2007).…”

“…Biological activities of recombinant eFSH and eLH were confirmed by in vitro oocyte maturation and in vivo spermatogenesis (Kobayashi et al, 2010).…”

“…Using baculovirus, recombinant Japanese eel (e) FSH and LH were produced in silkworm larvae as a biologically active, singlechain polypeptide (Kobayashi et al, 2010). Biological activities of recombinant eFSH and eLH were confirmed by in vitro oocyte maturation and in vivo spermatogenesis (Kobayashi et al, 2010).…”

CARACTERIZACIÓN BIOLÓGICA DE LAS GONADOTROFINAS DE LUBINA (Dicentrarchus labrax) Y DESARROLLO DE HERRAMIENTAS BIOTECNOLÓGICAS E INMUNOLÓGICAS PARA SU ESTUDIO

“…Other studies in fish had suggested that insect-produced GTHs could be rapidly cleared from circulation [13,60], but this has not been demonstrated or quantified. In this report, CHO-scGTHs showed a higher stability in plasma than Sf9-GTHs, which could be based in their different glycosylation because the content in terminal sialic acid residues can be a determinant for the rate at which glycoproteins are cleared from circulation [40,41,61].…”

“…Algunos estudios en peces sugieren que las GTHs recombinantes producidas en células de insecto podrían ser eliminadas rápidamente de la circulación sanguínea Kobayashi et al, 2010a), aunque no ha sido demostrado ni cuantificado. Por ello, en nuestro caso, antes de realizar experimentos in vivo decidimos estudiar la estabilidad en sangre y conocer la farmacocinética de las GTHs recombinantes producidas.…”