Production of recombinant human lysosomal acid lipase in Schizosaccharomyces pombe: Development of a fed-batch fermentation and purification process

Ikeda, Soichiro; Nikaido, Kiyokazu; Araki, Kyosuke; Yoshitake, Akifumi; Kumagai, Hiromichi; Isoai, Atsushi

doi:10.1016/s1389-1723(04)00297-x

Cited by 24 publications

(7 citation statements)

References 21 publications

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“…Furthermore, the physiology of Sc. pombe (such as its cell cycle, chromosome structure, and RNA splicing) often shows a high similarity to more complex eukaryotic cells and can therefore be used to express heterologous genes from higher eukaryotes (Giga-Hama et al ., 1994; Ikeda et al ., 2004). However, for heterologous expression of most high eukaryotic genes, Ko.…”

Section: Genetic Modificationmentioning

confidence: 99%

Improving industrial yeast strains: exploiting natural and artificial diversity

Steensels¹,

Snoek²,

Meersman³

et al. 2014

FEMS Microbiol Rev

398

288

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Yeasts have been used for thousands of years to make fermented foods and beverages, such as beer, wine, sake, and bread. However, the choice for a particular yeast strain or species for a specific industrial application is often based on historical, rather than scientific grounds. Moreover, new biotechnological yeast applications, such as the production of second-generation biofuels, confront yeast with environments and challenges that differ from those encountered in traditional food fermentations. Together, this implies that there are interesting opportunities to isolate or generate yeast variants that perform better than the currently used strains. Here, we discuss the different strategies of strain selection and improvement available for both conventional and nonconventional yeasts. Exploiting the existing natural diversity and using techniques such as mutagenesis, protoplast fusion, breeding, genome shuffling and directed evolution to generate artificial diversity, or the use of genetic modification strategies to alter traits in a more targeted way, have led to the selection of superior industrial yeasts. Furthermore, recent technological advances allowed the development of high-throughput techniques, such as ‘global transcription machinery engineering’ (gTME), to induce genetic variation, providing a new source of yeast genetic diversity.

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Section: Genetic Modificationmentioning

confidence: 99%

Improving industrial yeast strains: exploiting natural and artificial diversity

Steensels¹,

Snoek²,

Meersman³

et al. 2014

FEMS Microbiol Rev

398

288

View full text Add to dashboard Cite

show abstract

“…The methylotrophic yeast Pichia pastoris has been most widely used, e.g., for production of lysosomal acid lipase, α-galactosidase, glucocerebrosidase, and several other enzymes [213–217]. Ogatea minuta, Yarrowia lipolytica, Saccharomyces cerevisiae, and Schizosaccharomyces pombe have also been used [218–226]. Bacteria, in particular strains of Escherichia coli , have also been employed for this purpose, yet formation of protein aggregates as inclusion bodies and lack of post-translational modifications severely limit their applicability in producing recombinant enzymes for lysosomal ERT [227–231].…”

Section: Lysosomal Enzyme Replacement Therapymentioning

confidence: 99%

Lysosomal enzyme replacement therapies: Historical development, clinical outcomes, and future perspectives

Solomon

Muro

2017

Advanced Drug Delivery Reviews

122

113

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Lysosomes and lysosomal enzymes play a central role in numerous cellular processes, including cellular nutrition, recycling, signaling, defense, and cell death. Genetic deficiencies of lysosomal components, most commonly enzymes, are known as “lysosomal storage disorders” or “lysosomal diseases” (LDs) and lead to lysosomal dysfunction. LDs broadly affect peripheral organs and the central nervous system (CNS), debilitating patients and frequently causing fatality. Among other approaches, enzyme replacement therapy (ERT) has advanced to the clinic and represents a beneficial strategy for 8 out of the 50–60 known LDs. However, despite its value, current ERT suffers from several shortcomings, including various side effects, development of “resistance”, and suboptimal delivery throughout the body, particularly to the CNS, lowering the therapeutic outcome and precluding the use of this strategy for a majority of LDs. This review offers an overview of the biomedical causes of LDs, their socio-medical relevance, treatment modalities and caveats, experimental alternatives, and future treatment perspectives.

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“…High specific growth rate has decreased lipase production in the latter part of fed-batch cultures, due to build-up of excess oleic acid. A fed-batch fermentation process was developed [184] to enable the production of large quantities of recombinant human lysosomal acid lipase in Schizosaccharomyces pome. A feedback fed-batch system was used to determine the optimal feed rate of 50% glucose solution as carbon source.…”

Section: Fed Batch Processesmentioning

confidence: 99%

Overview of Fungal Lipase: A Review

Singh

Mukhopadhyay

2011

Appl Biochem Biotechnol

343

200

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Lipases (triacylglycerolacyl hydrolases, EC3.1.1.3) are class of enzymes which catalyze the hydrolysis of long-chain triglycerides. In this review paper, an overview regarding the fungal lipase production, purification, and application is discussed. The review describes various industrial applications of lipase in pulp and paper, food, detergent, and textile industries. Some important lipase-producing fungal genera include Aspergillus, Penicillium, Rhizopus, Candida, etc. Current fermentation process techniques such as batch, fed-batch, and continuous mode of lipase production in submerged and solid-state fermentations are discussed in details. The purification of lipase by hydrophobic interaction chromatography is also discussed. The development of mathematical models applied to lipase production is discussed with special emphasis on lipase engineering.

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Production of recombinant human lysosomal acid lipase in Schizosaccharomyces pombe: Development of a fed-batch fermentation and purification process

Cited by 24 publications

References 21 publications

Improving industrial yeast strains: exploiting natural and artificial diversity

Improving industrial yeast strains: exploiting natural and artificial diversity

Lysosomal enzyme replacement therapies: Historical development, clinical outcomes, and future perspectives

Overview of Fungal Lipase: A Review

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