Production of Recombinant Human Lactoferin in Transgenic Barley

Kamenarova, Kunka; Gecheff, K.; Stoyanova, Mariya; Muhovski, Yordan; Anzai, Hiroyuki; Atanassov, A.

doi:10.1080/13102818.2007.10817407

Cited by 25 publications

(16 citation statements)

References 90 publications

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“…In fact, from a practical point of view, agronomically superior cultivars adapted to the region should only be used despite their recalcitrance to tissue culture methods in order to achieve gains in terms of production and quality. A few successful reports are available for commercially important barley varieties including the Finnish elite cultivar Kymppi (Ritala et al 1994 ;Nuutila et al 1999 ); several German spring cultivars (Koprek et al 1996 ;Sharma et al 2006 ); the North American cultivars, Harrington, Galena and Conlon (Cho et al 1998 ;Zhang et al 1999 ;Manoharan and Dahleen 2002 ;Manoharan et al 2006 ;Tobias et al 2007 ); the elite Australian barley cultivars, Schooner, Chebec and Sloop Murray et al 2004 ); and a Bulgarian winter barley (Kamenarova et al 2007 ). However, in all these studies transformation effi ciency was reported to be low.…”

Section: Genotype Dependencymentioning

confidence: 91%

“…Transgenic barley plants expressing human lactoferrin ( hLF ) were also described from other groups. Western blot analysis of leaf tissue from T 0 plants documented the expression of the recombinant human lactoferrin (Kamenarova et al 2007 ), whereas Tanasienko et al ( 2011 ) provided proof for the presence of the gene hLF fragment in leaves of T 0 plants by PCR. Eskelin et al ( 2009 ) and Erlendsson et al ( 2010 ) presented further convincing evidences that transgenic barley seeds can be utilised as a bioreactor.…”

Section: Barley Grains As a Bioreactormentioning

confidence: 99%

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Barley

Schulze¹

2013

Alien Gene Transfer in Crop Plants, Volume 2

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Barley ( Hordeum vulgare L.) is a widely adapted cereal crop with an extremely wide geographic distribution throughout the world. It fi nds great use for animals as a feed and for humans as a grain, especially as the source for malt for the brewing industry. In recent times, there is considerable interest in the nutritional properties of barley due to the discovery of the cholesterol-lowering effect of β-glucan, a cell wall polysaccharide. Exploitation of genetic diversity in the primary and secondary gene pool of barley using DNA-based technologies has yielded interspecifi c crosses with improved grain properties, malting quality and resistance to biotic and abiotic stresses. The signifi cant achievements regarding introgression of alien genes include the genes Rym14(Hb) , Rym16(Hb) and Ryd4(Hb) which were introgressed from Hordeum bulbosum conferring resistance to BaMMV, BaYMV and BYDV in barley. Signifi cant advances in genetic engineering of barley have been obtained, and strategies for establishment of regenerative cell and tissue culture systems as well as for development of DNA delivery techniques have been formulated. Lately, a huge potential has been realised in barley grains to produce pharmaceutical proteins like oral vaccines, growth supplements and food additives which are being exploited in a commercial scale. Nevertheless, several problems still remain like the strong genotype dependency of barley transformation protocols, transformation effi ciency, transgene stability and public acceptance. The review focuses on all these issues and elaborates achievements made in the last two decades in genetic enhancement of barley using different alien gene transfer approaches.

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Section: Genotype Dependencymentioning

confidence: 91%

Section: Barley Grains As a Bioreactormentioning

confidence: 99%

Barley

Schulze¹

2013

Alien Gene Transfer in Crop Plants, Volume 2

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“…Despite its numerous benefits, rice has not been cultivated traditionally in many countries. Since the amount of rhLf protein accumulation reached only 3 ng/mg of total soluble protein (Kamenarova et al, 2007), the range of other commercial barley cultivars were tested (Tanasienko et al, 2009;Yemets et al, 2012) and the novel selection system for the efficient genetic transformation was developed (Tanasienko et al, 2011;Yemets et al, 2012). Barley is grown over a broader range of environmental conditions than any other cereals and considered to be an important crop in the northern regions of North America and Europe (Ritala et al, 2008;Mrízová et al, 2014).…”

Section: Characteristics Of the Key Functions Of Lactoferrinmentioning

confidence: 99%

Plant‐based biopharming of recombinant human lactoferrin

Yemets

Tanasienko

Krasylenko

et al. 2014

Cell Biology International

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Recombinant proteins are currently recognized as pharmaceuticals, enzymes, food constituents, nutritional additives, antibodies and other valuable products for industry, healthcare, research, and everyday life. Lactoferrin (Lf), one of the promising human milk proteins, occupies the expanding biotechnological food market niche due to its important versatile properties. Lf shows antiviral, antimicrobial, antiprotozoal and antioxidant activities, modulates cell growth rate, binds glycosaminoglycans and lipopolysaccharides, and also inputs into the innate/specific immune responses. Development of highly efficient human recombinant Lf expression systems employing yeasts, filamentous fungi and undoubtedly higher plants as bioreactors for the large-scale Lf production is a biotechnological challenge. This review highlights the advantages and disadvantages of the existing non-animal Lf expression systems from the standpoint of protein yield and its biological activity. Special emphasis is put on the benefits of monocot plant system for Lf expression and the biosafety aspects of the transgenic Lf-expressing plants.

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“…Human Lf is already successfully produced in various crops -tobacco (Liu et al 1996, Salmon et al 1998, Zhang et al 1998, potato (Chong and Langridge 2000), tomato (Anzai et al 2000, Lee et al 2002, rice (Anzai et al 2000, Nandi et al 2002, Takase et al 2005, maize (Samyn-Petit et al 2001), barley (Kamenarova et al 2007), ginseng . High expression levels for the use of pharmacy can be obtained by using strong promoters and direction of protein accumulation in different parts of the plant -fruits, seeds, leaves and tubers.…”

Section: Introductionmentioning

confidence: 97%

Production of recombinant human lactoferrin from transgenic plants

2008

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Molecular farming provides a powerful tool for low cost production of recombinant proteins with pharmaceutical value. The use of transgenic plants has been increasingly tested as alternative system for obtaining biologically active human lactoferrin in plants. Precise selection of plant species, transformation techniques and expression cassettes, in addition to conduction of detailed glycosylation and immunogenicity studies, serves as basis of obtaining safe recombinant human lactoferrin in high concentrations for the use of pharmacy. On the other hand, expression of antimicrobial protein lactoferrin in plants is a promising opportunity for crop quality improvement by increasing plant disease resistance.

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Production of Recombinant Human Lactoferin in Transgenic Barley

Cited by 25 publications

References 90 publications

Barley

Barley

Plant‐based biopharming of recombinant human lactoferrin

Production of recombinant human lactoferrin from transgenic plants

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