2011
DOI: 10.1590/s1984-29612011000100011
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Production of recombinant EMA-1 protein and its application for the diagnosis of Theileria equi using an enzyme immuno assay in horses from São Paulo State, Brazil

Abstract: The erythrocytic-stage surface protein, Equi Merozoite Antigen 1 (EMA-1), is a major candidate for the development of a diagnostic antigen for equine piroplasmosis. In order to establish an effective diagnostic method for practical use, the gene encoding the entire EMA-1 of Theileria equi Jaboticabal strain was cloned and expressed in Escherichia coli as a histidine-tagged protein (His6-EMA1). The expressed EMA-1 reacted with specific antibodies in Western blot and had an apparent molecular mass of 34 … Show more

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Cited by 10 publications
(9 citation statements)
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“…The use of a single epitope also reduces the chance of cross-reactivity between the parasites. The immunodominant T. equi surface antigens equine merozoite antigen (ema)-1 and ema-2, and the B. caballi rhoptry-associated protein (rap)-1 were successfully used and proven superior to IFAT and CFT in several studies [2,3,5,20,30,68,74,76,77,[79][80][81]. Nevertheless, some heterogeneity has been recorded between isolates, and the USDA-approved B. caballi rap-1 cELISA assay did not detect infected horses in South-Africa and in the Middle East [82][83][84].…”
Section: Diagnosismentioning
confidence: 99%
See 1 more Smart Citation
“…The use of a single epitope also reduces the chance of cross-reactivity between the parasites. The immunodominant T. equi surface antigens equine merozoite antigen (ema)-1 and ema-2, and the B. caballi rhoptry-associated protein (rap)-1 were successfully used and proven superior to IFAT and CFT in several studies [2,3,5,20,30,68,74,76,77,[79][80][81]. Nevertheless, some heterogeneity has been recorded between isolates, and the USDA-approved B. caballi rap-1 cELISA assay did not detect infected horses in South-Africa and in the Middle East [82][83][84].…”
Section: Diagnosismentioning
confidence: 99%
“…The choice of these loci as targets for molecular investigation was based on the immunodominant properties of the corresponding proteins. Both EMA-1 and EMA-2 proteins have been used as antigens in serologic tests [30,76,79,95,257], and ema-1 has been used as a target for molecular screening [87,95]. For these purposes, the conserved nature of these genes and antigens was advantageous, in order to develop robust diagnostic techniques.…”
Section: Theileria Equi Ema Genotypes and Their Global Distributionmentioning
confidence: 99%
“…Equine theileriosis stands out as a major cause of both horse illness and of losses to the horse raising industry. Even with improvements in health, piroplasmosis causes losses associated with clinical factors and through international restrictions on the transit of seropositive animals [7,8]. Theileria equi is small, 2-3 mm in length [9], pleomorphic within erythrocytes, and is isolated in pairs or when forming tetrads (Maltese cross).…”
Section: Introductionmentioning
confidence: 99%
“…These parasites are widely distributed in the world, including Asian continent, Europe, Africa, and South America, and the prevalence corresponds to the presence of the tick-vectors De Waal 1992). Equine merozoite surface antigens (EMAs) have been utilized as diagnostic antigens in many ELISAs (Knowles et al 1992;Baldani et al 2011;Huang et al 2003;Tanaka et al 1999;Xuan et al 2001). PCR has been found more efficient and sensitive in detecting the T. equi latent infection and several PCR methodologies have been described and used in diagnosis of the infection (Bashiruddin et al 1999;Nicolaiewsky et al 2001;Battasetseg et al 2001;Rampersad et al 2003).…”
Section: Introductionmentioning
confidence: 99%