Production of recombinant botulism antigens: A review of expression systems

Moreira, Gustavo Marçal Schmidt Garcia; Cunha, C. E.; Salvarani, Felipe Masiero; Gonçalves, Luciana Aramuni; Pires, Prhiscylla Sadanã; Conceição, Fabrı́cio R.; Lobato, Francisco Carlos Faria

doi:10.1016/j.anaerobe.2014.06.003

Cited by 31 publications

(26 citation statements)

References 83 publications

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“…The symptoms observed in the affected buffaloes, including sudden death, sternal recumbency, inability to stand, and flaccid paralysis, were similar to those described in botulism outbreaks in ruminants, and the absence of macroscopic and microscopic pathological changes corroborated the results of previous studies, which reported the lack of major changes or the presence of mild changes (Senturk & Cihan 2007, Myllykoski et al 2009, Moreira et al 2014.…”

Section: Discussionsupporting

confidence: 79%

“…Botulism in animal production has a strong economic impact due to the high mortality rates (Moreira et al 2014). The Food and Agriculture Organization of the United Nations (2014) reported that the population of buffaloes (Bubalus bubalis) worldwide is approximately 195 million heads.…”

Section: Introductionmentioning

confidence: 99%

“…The period of incubation and disease severity depends on the amount of toxin ingested and the animal's sensitivity to BoNTs. The course of the disease varies from a few hours to a few days, and the mortality rate is close to 100% (Moreira et al 2014).…”

Section: Introductionmentioning

confidence: 99%

“…During the course of the disease, the psychological condition of the animal remains unchanged. Alterations at necropsy are negligible and limited to petechiae in the myocardium due to the respiratory distress that precedes death (Moreira et al 2014). Definitive diagnosis is based on the detection of BoNTs using a mouse bioassay (Bano et al 2015) which is the gold standard test for the detection and characterization of botulinum toxins (Lindström & Korkeala 2006).…”

Section: Introductionmentioning

confidence: 99%

“…BoNTs are produced by Clostridium botulinum, a gram-positive obligatory anaerobic bacterium that survives for long periods under adverse environmental conditions in its spore form. Under specific anaerobic conditions, assumes a vegetative form, multiplies, and secretes BoNTs (Gil et al 2013, Moreira et al 2014. BoNTs are the most toxic biological substances known and are classified into eight serotypes (A-H) on the basis of their antigenic properties.…”

Section: Introductionmentioning

confidence: 99%

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Type C waterborne botulism outbreaks in buffaloes (Bubalus bubalis) in the Amazon region

et al. 2017

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Botulism is a poisoning caused by botulinum neurotoxins (BoNTs). BoNTs serotypes C and D are involved in botulism outbreaks in cattle in several countries. Despite the high number of buffaloes worldwide, the real impact of botulism in buffaloes is not known, because it is not a notifiable disease in Brazil and only few studies have evaluated the occurrence of the disease in buffaloes. Those studies did not conduct diagnostic tests to confirm the presence of BoNTs. The objective of the present study was to describe three outbreaks of botulism in buffaloes in the Brazilian Amazon region considering epidemiological and clinical data as well as laboratory diagnosis to confirm the presence of BoNTs. The results of the bioassay were negative in the tissues and in feed samples, but positive for BoNT C in water samples. Confirmation of the occurrence of botulism in buffaloes allows the implementation of preventive strategies in susceptible herds. Waterborne botulism in buffaloes is prevented by ensuring the constant circulation of water collections and restricting the presence of dead animals and bones in order to prevent the accumulation of organic matter and the development of anaerobic conditions, which might favor the replication of Clostridium botulinum. Another measure that can be adopted is the shading of the pasture, in order to maintain the thermal comfort for the buffaloes and to avoid the excess of permanence of them in the water pools.

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Section: Discussionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%