Production of pyruvate from Ulva reticulata using the alkaliphilic, halophilic bacterium Halomonas sp. BL6

Anh, Hoàng Thị Lan; Kawata, Yoshikazu; Tam, Luu Thi; Thom, Le Thi; Ha, Nguyen Cam; Hien, Hoang Thi Minh; Thu, Ngo Thi Hoai; Huy, Pham Quang; Hồng, Đặng Diễm

doi:10.1007/s10811-020-02035-1

Cited by 14 publications

(8 citation statements)

References 50 publications

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“…Pyruvate is an essential pharmaceutical intermediate, which has been widely used as a starting material in the biosynthesis of pharmaceuticals, such as L-tryptophan, L-tyrosine, alanine, and L-DOPA [110]. Most of the reported studies produce pyruvate from glucose, and studies in the literature regarding the production of pyruvate from other sources are rare, especially alginate [111]. During the process of generating pyruvate, DEH produced by exolytic alginate lyases can be reduced to 2-keto-3-deoxy-D-gluconate (KDG) which enters the Entner−Doudoroff pathway to produce two molecules of pyruvate [112].…”

Section: Preparation Of Pharmaceutical Intermediatementioning

confidence: 99%

Structure Characteristics, Biochemical Properties, and Pharmaceutical Applications of Alginate Lyases

et al. 2021

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Alginate, the most abundant polysaccharides of brown algae, consists of various proportions of uronic acid epimers α-L-guluronic acid (G) and β-D-mannuronic acid (M). Alginate oligosaccharides (AOs), the degradation products of alginates, exhibit excellent bioactivities and a great potential for broad applications in pharmaceutical fields. Alginate lyases can degrade alginate to functional AOs with unsaturated bonds or monosaccharides, which can facilitate the biorefinery of brown algae. On account of the increasing applications of AOs and biorefinery of brown algae, there is a scientific need to explore the important aspects of alginate lyase, such as catalytic mechanism, structure, and property. This review covers fundamental aspects and recent developments in basic information, structural characteristics, the structure–substrate specificity or catalytic efficiency relationship, property, molecular modification, and applications. To meet the needs of biorefinery systems of a broad array of biochemical products, alginate lyases with special properties, such as salt-activated, wide pH adaptation range, and cold adaptation are outlined. Withal, various challenges in alginate lyase research are traced out, and future directions, specifically on the molecular biology part of alginate lyases, are delineated to further widen the horizon of these exceptional alginate lyases.

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Section: Preparation Of Pharmaceutical Intermediatementioning

confidence: 99%

Structure Characteristics, Biochemical Properties, and Pharmaceutical Applications of Alginate Lyases

et al. 2021

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“…Ulva reticulata is edible green seaweed with a high content of minerals, vitamins, and other phytoconstituents. Its balanced amino acid profile makes this a potential dietary alternative [27,28]. This seaweed is widely distributed in Asian countries and consumed as fresh, dried ingredients in prepared foods [27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

“…Its balanced amino acid profile makes this a potential dietary alternative [27,28]. This seaweed is widely distributed in Asian countries and consumed as fresh, dried ingredients in prepared foods [27][28][29][30]. As part of our ongoing research on seaweeds, this study describes the antidiabetic effects (α-amylase, α-glucosidase, and DPP-IV inhibition) of Ulva reticulata followed by bioactivity-guided isolation and characterization of compounds using chromatographic and spectroscopic techniques.…”

Section: Introductionmentioning

confidence: 99%

Bioactivity Guided Study for the Isolation and Identification of Antidiabetic Compounds from Edible Seaweed—Ulva reticulata

et al. 2022

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Managing diabetes is challenging due to the complex physiology of the disease and the numerous complications associated with it. As part of the ongoing search for antidiabetic chemicals, marine algae have been demonstrated to be an excellent source due to their medicinal properties. In this study, Ulva reticulata extracts were investigated for their anti-diabetic effect by examining its inhibitory effects on α-amylase, α-glucosidase, and DPP-IV and antioxidant (DPPH) potential in vitro and its purified fraction using animal models. Among the various solvents used, the Methanolic extract of Ulva reticulata (MEUR) displayed the highest antidiabetic activity in both in vitro and in vivo; it showed no cytotoxicity and hence was subjected to bioassay-guided chromatographic separation. Among the seven isolated fractions (F1 to F7), the F4 (chloroform) fraction exhibited substantial total phenolic content (65.19 μg mL−1) and total flavonoid content (20.33 μg mL−1), which showed the promising inhibition against α-amylase (71.67%) and α-glucosidase (38.01%). Active fraction (F4) was further purified using column chromatography, subjected to thin-layer chromatography (TLC), and characterized by spectroscopy techniques. Upon structural elucidation, five distinct compounds, namely, Nonane, Hexadecanoic acid, 1-dodecanol, Cyclodecane methyl, and phenol, phenol (3,5-bis(1,1-dimethylethyl) were identified. The antidiabetic mechanism of active fraction (F4) was further investigated using various in vitro and in vivo models. The results displayed that in in vitro both 1 and 24 h in vitro cultures, the active fraction (F4) at a concentration of 100 μg mL−1 demonstrated maximum glucose-induced insulin secretion at 4 mM (0.357 and 0.582 μg mL−1) and 20 mM (0.848 and 1.032 μg mL−1). The active fraction (F4) reduces blood glucose levels in normoglycaemic animals and produces effects similar to that of standard acarbose. Active fraction (F4) also demonstrated outstanding hypoglycaemic activity in hyperglycemic animals at a dose of 10 mg/kg B.wt. In the STZ-induced diabetic rat model, the active fraction (F4) showed a (61%) reduction in blood glucose level when compared to the standard drug glibenclamide (68%). The results indicate that the marine algae Ulva reticulata is a promising candidate for managing diabetes by inhibiting carbohydrate metabolizing enzymes and promoting insulin secretion.

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“… 2017 ). In the cosmetic field, pyruvic acid is used for anti-acne and anti-skin aging treatment (Anh et al 2020 ). Pyruvic acid is also an important metabolic intermediate compound linking microbial nitrogen metabolism and carbon metabolism.…”

Section: Introductionmentioning

confidence: 99%

Production of pyruvic acid with Candida glabrata using self-fermenting spent yeast cell dry powder as a seed nitrogen source

Shan

Zeng

et al. 2022

Bioresour. Bioprocess.

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Pyruvic acid is an important organic acid and a key industrial raw material. It is widely used in the chemical, agricultural, and food fields. Candida glabrata is the preferred strain for pyruvic acid production. The waste yeast cell for pyruvic acid fermentation with C. glabrata are rich in protein, amino acid, nucleic acid, and vitamins, as potential and cost-effective nitrogen source raw material. In this study, the potential of C. glabrata to produce pyruvic acid using spent yeast cell dry powder was evaluated. When 30 g/L of spray-dried spent yeast cell powder was used as the seed nitrogen source, a high titer of pyruvic acid was obtained. The pyruvic acid production reached 63.4 g/L with a yield of 0.59 g/g in a 5 L bioreactor. After scale-up to a 50 L bioreactor using the fermented spent yeast cell dry powder as a seed nitrogen source, 65.1 g/L of pyruvic acid was harvested, with a yield of 0.61 g/g. This study proposes a promisingapproach for increasing the pyruvic acid titer and reducing the costs. Graphical Abstract

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Production of pyruvate from Ulva reticulata using the alkaliphilic, halophilic bacterium Halomonas sp. BL6

Cited by 14 publications

References 50 publications

Structure Characteristics, Biochemical Properties, and Pharmaceutical Applications of Alginate Lyases

Structure Characteristics, Biochemical Properties, and Pharmaceutical Applications of Alginate Lyases

Bioactivity Guided Study for the Isolation and Identification of Antidiabetic Compounds from Edible Seaweed—Ulva reticulata

Production of pyruvic acid with Candida glabrata using self-fermenting spent yeast cell dry powder as a seed nitrogen source

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