Production of protoplasts from Fusarium scirpi by lytic enzymes from Streptomyces tsusimaensis

D-Lysergyl Peptide Synthetase from the Ergot Fungus Claviceps purpurea

“…It would therefore be an appropriate target for the observed repression of ergotamine synthesis in C. purpurea wild type in Vogel's medium (12). In the cases of enniatin synthetase in Fusarium scirpi, the producer of the cyclohexadepsipeptide enniatin B and of other toxins of phytopathogenic fungi, constitutive peptide synthetase expression has also been demonstrated (45). It may be argued that the constitutive expression of peptide synthetases may allow rapid conversion of key metabolites such as D-lysergic acid into the peptide alkaloids.…”

Piriformospora indica: An Axenically Culturable Mycorrhiza-Like Endosymbiotic Fungus

“…Protoplasts can be used for various purposes, including the investigation of cell wall synthesis and synchronised cell growth (Davis 1985), for the isolation of cytoplasmic organelles (Billich et al 1988), for cell fusion (Minuth and Esser 1983), for the preparation of high molecular weight DNA for cloning and karyotyping (Barrett et al 1989), and as a source material for mutant induction as well as for transformation experiments designed for strain improvement (Hynes 1986;Hampp et al 1998). Until recently, all these experiments seemed a distant possibility for manipulating AM fungi due to the inability to culture them axenically.…”