Production of Protein Hydrolysates Using Marine Catfish Bagre panamensis Muscle or Casein as Substrates: Effect of Enzymatic Source and Degree of Hydrolysis on Antioxidant and Biochemical Properties

Rios-Herrera, Gissel Daniela; Salazar-Leyva, Jesús Aarón; Hernández, Crisantema; Jiménez-Gutiérrez, Laura Rebeca; Sandoval-Gallardo, Jorge Manuel; Osuna-Ruíz, Idalia; Martínez‐Montaño, Emmanuel; Pacheco‐Aguilar, Ramón; Lugo‐Sánchez, María Elena; Ramírez‐Pérez, Jorge Saúl

doi:10.1007/s12010-021-03603-y

Cited by 4 publications

(10 citation statements)

References 39 publications

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“…Additionally, Rios‐Herrera et al. [ 1 ] showed that DPPH activity was increased by increased hydrolysis of catfish Bagre panamensis muscle hydrolysates, and that DH was associated with DPPH radical scavenging activity in a significant, positive and favorable manner ( P < 0.001). The presence of low‐molecular‐weight peptides in hydrolyzed proteins is associated with a significant increase in antioxidant activity, which is well known to be affected by the hydrolysis degree.…”

Section: Resultsmentioning

confidence: 99%

“…This result was in agreement with the reported results by Foh et al [22] who have used three enzymes including Alcalase 2.4 L, Neutrase, and Flavourzyme to hydrolyze tilapia (Oreochromis niloticus) minced meat, and they stated that the hydrolysates with the higher degree of hydrolysis showed better DPPH scavenging activity. Additionally, Rios-Herrera et al [1] showed that DPPH activity was increased by increased hydrolysis of catfish Bagre panamensis muscle hydrolysates, and that DH was associated with DPPH radical scavenging activity in a significant, positive and favorable manner (P < 0.001). The presence of low-molecular-weight peptides in hydrolyzed proteins is associated with a significant increase in antioxidant activity, which is well known to be affected by the hydrolysis degree.…”

Section: Dpph • Radical Scavenging Activitymentioning

confidence: 99%

Section: Dpph • Radical Scavenging Activitymentioning

confidence: 99%

“…In recent decades, aquaculture and seafood processing has been receiving great attention from industry and researchers to support the protein demands of an increasing human population. [1,2] Consequently, a massive amount of by-products are generated after seafood processing, including heads, tails, fins, spines, viscera, and skin, which are responsible for 75% of the properties related to their amino acid content and sequence will be generated. [3] These two factors will also affect the possible bioactive properties of the recovered protein hydrolysates.…”

Section: Introductionmentioning

confidence: 99%

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Fish Protein Hydrolysate from Sulfated Polysaccharides Extraction Residue of Tuna Processing By‐Products with Bioactive and Functional Properties

2023

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However, these by-products are a great source to extract bioactive compounds including protein hydrolysates, sulfated polysaccharide, EPA, and DHA with numerous bioactive properties such as antioxidant, antibacterial, antihypertensive, angiotensin I-converting enzyme inhibitory. [3] To extract these natural products from fish by-products, many strategies have been developed in recent years, such as enzyme-assisted extraction, ultrasonicassisted extraction, microwave-assisted extraction, accelerated solvent extraction, pressurized water extraction, and ultrahigh-pressure extraction. [1,2] For instance, the first goal of an enzymatic hydrolysis method is to obtain high-quality protein fragments called hydrolysate with functional properties, but enzymatic hydrolysis under optimized conditions can generate protein hydrolysates with bioactive properties. [3] However, more recently the enzymatic hydrolysis process has been used for the destruction of connections between protein-polysaccharides for extraction of sulfated polysaccharides from fish by-products. This initial enzymatic hydrolysis step is then followed with the ethanol or cetylpyridinium chloride (CPC) induced precipitation to recover the extracted polysaccharides as precipitate. [4] Consequently, after polysaccharides precipitation which normally encompasses a very small portion of marine biomass, a huge volume of ethanol or CPC remains and is discarded while it still contains hydrolyzed proteins that could be recovered from the ethanol phase. This will provide a unique opportunity for parallel extraction of polysaccharides and protein hydrolysate from fish products. Co-extraction of these two products can increase the overall profitability of the process by increasing product diversity and revenue. It also enables more efficient utilization of marine resources by taking care of side streams. In this context, Abdollahi et al. [5] developed a biorefinery concept for sequential recovery of collagen and collagen hydrolysate from the sediment residue created during pH-shiftbased protein isolation from silver carp (Hypophthalmichthys molitrix). However, as far as we know, parallel extraction of protein hydrolysates from waste streams generated during the extraction of sulfated polysaccharides from fish processing byproducts has not been documented.In addition, depending on their origin and extraction process, fish protein hydrolysates (FPH) with various nutritional The ethanol-induced precipitation after enzymatic hydrolysis commonly used for sulfated polysaccharide extraction from marine resources wastes a large amount of proteins. Here, possible extraction of fish protein hydrolysates (FPH) from the ethanol residue of sulfated polysaccharide precipitation from head, bone, and skin of skipjack tuna is investigated. Antioxidant, antibacterial, angiotensin I-converting enzyme (ACE) inhibitory activities and functional properties of the recovered FPHs are also evaluated. A degree of hydrolysis of 40.93, 38.13, and 37.23 is achieved for FPH from head, bone, and ski...

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Section: Resultsmentioning

confidence: 99%

Section: Dpph • Radical Scavenging Activitymentioning

confidence: 99%

Section: Dpph • Radical Scavenging Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fish Protein Hydrolysate from Sulfated Polysaccharides Extraction Residue of Tuna Processing By‐Products with Bioactive and Functional Properties

2023

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“…The degree of hydrolysis (DH) value was measured using the "pHstat" method with a few modifications (Rios-Herrera et al, 2021). Specifically, the Pheretima vulgaris protein (0.1 g) was dissolved in 10 ml ultrapure water and digested by each enzyme under the optimal condition.…”

Section: Analysis Of the Degree Of Hydrolysis Valuementioning

confidence: 99%

Purification and identification of thrombolytic peptides from enzymatic hydrolysate of Pheretima vulgaris

Feng

Zhang

Wang

et al. 2022

Journal of Food Biochemistry

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Pheretima vulgaris has been prescribed for the treatment of cardiovascular diseases in China for several hundred years in the form of dried powder in the clinic. However, the peptides with the potential antithrombotic activity of this source have never been reported. The total active proteins from Pheretima vulgaris were hydrolyzed by eight different commercial proteases and the alcalase hydrolysate showed the strongest thrombolytic activity. Four original thrombolytic peptides were isolated and characterized using bioactivity‐directed fractionation of the active hydrolysate. The amino acid sequences were identified as HEPLPEP (m/z 818.40076), EYPLPEP (m/z 844.39648), LGEPSVP (m/z 698.39648), and LLAPP (m/z 510.28043) by nanoLC‐ESI‐Orbitrap mass spectrometry with PEAKS software. HEPLPEP and EYPLPEP, containing the common ‐PLPEP residue, showed superior thrombolytic activity in plasmin assay and fibrinogen‐thrombin time assay. This research confirmed that Pheretima vulgaris was a potential source of active peptides with thrombolytic activities and provided novel candidates for the thrombolytic agents. Practical applications Thrombosis has become the leading cause of mortality as it was the common underlying pathology of cardiovascular diseases, such as ischemic heart disease, and stroke. The demand for thrombolytics has increased gradually as the incidence trends of thrombosis‐related diseases raise with the aging of the population. Four novel thrombolytic peptides were characterized from Pheretima vulgaris proteins hydrolysates, among which HEPLPEP and EYPLPEP could prevent the formation of thrombus and degrade existing thrombus in vitro. These peptides are promising to be meritorious templates for developing thrombolytic agents. The structure–function relationship of peptides resulting from the presence of specific residues in these sequences may contribute to extending the knowledge about their thrombolytic activity, which may be useful in designing novel thrombolytic agents. The present research based on a bioactivity‐directed isolation strategy could also be applied to other animal‐derived traditional Chinese medicines with proteins or peptides as their function basis.

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Free Radical Scavenging-Related Antioxidant Properties and Inhibitory Activities of Telfairia occidentalis Seed Protein Hydrolysates and Peptide Fractions Against Two key Enzymes Implicated in Diabetes Mellitus

Ruth

Idowu

Afolabi

et al. 2023

Int J Pept Res Ther

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Production of Protein Hydrolysates Using Marine Catfish Bagre panamensis Muscle or Casein as Substrates: Effect of Enzymatic Source and Degree of Hydrolysis on Antioxidant and Biochemical Properties

Cited by 4 publications

References 39 publications

Fish Protein Hydrolysate from Sulfated Polysaccharides Extraction Residue of Tuna Processing By‐Products with Bioactive and Functional Properties

Fish Protein Hydrolysate from Sulfated Polysaccharides Extraction Residue of Tuna Processing By‐Products with Bioactive and Functional Properties

Purification and identification of thrombolytic peptides from enzymatic hydrolysate of Pheretima vulgaris

Free Radical Scavenging-Related Antioxidant Properties and Inhibitory Activities of Telfairia occidentalis Seed Protein Hydrolysates and Peptide Fractions Against Two key Enzymes Implicated in Diabetes Mellitus

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