Production Of Protease By Submerged Fermentation Using Rhizopus Microsporus Var Oligospous

Sarao, Loveleen Kaur; Arora, Maninder; Sehgal, V. K.; Bhatia, Subhash

doi:10.5580/2582

Cited by 3 publications

(2 citation statements)

References 7 publications

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“…Similar research showed that the protease production method in submerged fermentation by Rhizopus microsporus var. oligosporus is optimal at pH 5.5 (Sarao et al, 2010). Previous research has revealed that the optimum pH of the media for protease synthesis from Rhizopus oligosporus IHS13 in sunflower meal, wheat bran, and rice bran is at pH 5.0.…”

Section: Resultsmentioning

confidence: 97%

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Fibrinolytic Protease Production: Impact of Initial pH and Temperature in Solid-State Fermentation by Rhizopus microsporus var. oligosporus FNCC 6010

Lusiana,

Poernomo,

Syahrani

2023

JFIKI

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Background: Fibrinolytic enzyme is one of the cardiovascular disease therapies. Rhizopus microsporus var. oligosporus is microorganism that has been evaluated to produce fibrinolytic protease by fermentation. This study conducted fermentation of helianthi annui semen (sunflower seed) by Rhizopus microsporus var. oligosporus to produce fibrinolytic enzyme. Objective: This study aims to determine the effect of Initial pH and incubation temperature and its optimization in the production of fibrinolytic protease by Rhizopus microsporus var. oligosporus FNCC 6010 in solid-state fermentation on helianthi annui semen (sunflower seed) substrate. Optimum condition was determined by maximum protease and fibrinolytic activity. Method: A crude enzyme of protease fibrinolytic was obtained from the supernatant extract of fermented sunflower seed. Protease activity was measured by the skimmed milk agar (SMA) plate method, and fibrinolytic activity was determined by the fibrin agar plate method. Result: It was found that the starting pH affects both the proteolytic and fibrinolytic activity of enzymes that are produced in fermentation. The starting pH of 5.0 showed higher fibrinolytic and proteolytic activity values compared to the starting pH of 7.0. The incubation temperature 33±1 °C had the higher activity compared to 28±1 °C or 37±1 °C. Conclusion: Initial pH and incubation temperature affect the proteolytic and fibrinolytic activity of crude enzyme extracted from fermented sunflower seed by Rhizopus microsporus var. oligosporus. The optimum condition for producing fibrinolytic protease in the state fermentation method was an initial pH of 5.0 and an incubation temperature of 33±1°C.

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Section: Resultsmentioning

confidence: 97%

“…A previous study reported that R. microsporus var. oligosporus made fibrinolytic enzymes by fermentation (Sarao et al, 2010). Other studies have revealed that Rhizopus species can produce fibrinolytic proteases, including R. oryzae (Sada et al, 2021); Rhizopus chinensis 12 (Xiao-Lan et al, 2005); R. microsporus var.…”

Section: Introductionmentioning

confidence: 99%

Fibrinolytic Protease Production: Impact of Initial pH and Temperature in Solid-State Fermentation by Rhizopus microsporus var. oligosporus FNCC 6010

Lusiana,

Poernomo,

Syahrani

2023

JFIKI

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Industrial Production and Optimization of Microbial Enzymes

Niyonzima

Veena²,

2020

Microorganisms for Sustainability

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Role of Melanin in Release of Extracellular Enzymes and Selection of Aggressive Isolates of Bipolaris sorokiniana in Barley

et al. 2014

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Eighteen barley isolates of Bipolaris sorokiniana belonging to wild and clonal type of black, mixed and white subpopulations were quantitatively assayed for their melanin content and aggressiveness with respect to production of some of the extracellular enzymes such as cellulase, pectinase, amylase and protease. Cellulase and pectinase constituted major portion of the enzymes recovered from the black, mixed and white isolates. Enzyme production and aggressiveness were relatively higher in melanin devoid or low melanin isolates. The melanin deficient isolates were also differentiated from black and mixed isolates on the basis of variation in internal transcribed spacer region of the ribosomal DNA. Higher enzyme productions positively correlated with area under disease progress curve (AUDPC) and lesion development. Melanin content was negatively correlated with extracellular enzymes and aggressiveness of the isolates. Based on melanin content, lesion size, AUDPC and extracellular enzymes, the isolates were grouped in two major clusters (I and II) with further division of cluster II into two sub-clusters (II-A and II-B). The results appears to indicate a possible role of melanin in release of extracellular enzymes and hence in evolution and selection of aggressive isolates of B. sorokiniana in barley.

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Production Of Protease By Submerged Fermentation Using Rhizopus Microsporus Var Oligospous

Cited by 3 publications

References 7 publications

Fibrinolytic Protease Production: Impact of Initial pH and Temperature in Solid-State Fermentation by Rhizopus microsporus var. oligosporus FNCC 6010

Fibrinolytic Protease Production: Impact of Initial pH and Temperature in Solid-State Fermentation by Rhizopus microsporus var. oligosporus FNCC 6010

Industrial Production and Optimization of Microbial Enzymes

Role of Melanin in Release of Extracellular Enzymes and Selection of Aggressive Isolates of Bipolaris sorokiniana in Barley

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