Production of proinflammatory and immunoregulatory cytokines by inflammatory cells from periapical lesions in culture

Gazivoda, Dragan; Džopalić, Tanja; Božić, Biljana; Tatomirovic, Zeljka; Brkić, Zlata; Čolić, Miodrag

doi:10.1111/j.1600-0714.2009.00788.x

Cited by 73 publications

(94 citation statements)

References 41 publications

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“…The morphological analysis showed that periapical granulomas exhibited higher grades of inflammatory infiltrate than the cysts, but this significant difference was not associated with the scores attributed to the immunohistochemical expression of TGF-β1 and MMP-9. In contrast, Gazivoda et al 10 used a fluorescent bead immunoassay and/or ELISA to prove that immunoregulatory cytokines such as TGF-β are more important for suppression of inflammation in asymptomatic lesions. Based on this finding, the effect of TGF-β was stronger than that of IL-10.…”

Section: Discussionmentioning

confidence: 99%

“…Substantial evidence suggests that these lesions are the result of a long-term endodontic infection or pulp necrosis leading to a progressive immune/inflammatory response, whose persistence in periapical tissues can result in the resorption of the surrounding bone and reactivation of epithelial cell rests of Malassez 10,21,22 ; which are probably the main source of the epithelial lining of radicular cysts. 9 In fact, it seems that this persistent, chronic inflammation and destruction of bone depends on the inability of a host defense to eradicate the infection.…”

Section: Discussionmentioning

confidence: 99%

“…9 In fact, it seems that this persistent, chronic inflammation and destruction of bone depends on the inability of a host defense to eradicate the infection. 10 The participants in this study were predominantly females, aged 20-40 years, and exhibited a well-demarcated radiolucency image, and asymptomatic lesions affecting the anterior maxilla. This patient profile was similar to that of several other recent epidemiological reports.…”

Section: Discussionmentioning

confidence: 99%

“…It performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, apoptosis and inflammation, as well as tissue repair. 10 Additionally, TGF-β1 immune/inflammatory functions include (a) chemotaxis for monocytes, neutrophils and fibroblasts; (b) initiation, growth and differentiation of inflammatory cells; and (c) formation of bone structures. 6 Several cells, such as neutrophils, macrophages, T cells, mast cells and odontoblasts, may secrete MMP-9, a metalloenzyme belonging the gelatinase group of matrix metalloproteinases (MMPs) that degrade a vast number of extracellular matrix (ECM) components, including denatured collagen, basement membrane and bone matrix.…”

Section: Declaration Of Interestmentioning

confidence: 99%

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Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions

et al. 2017

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The objective of this study was to evaluate the expression of matrix metalloproteinase 9 (MMP-9) and transforming growth factor beta (TGF-β1) in periapical lesion samples correlated with the intensity of the inflammatory infiltrate and thickness of the epithelial lining. Forty-five cases of periapical lesions (23 periapical granulomas and 22 radicular cysts) were subjected to morphological and immunohistochemical analyses using anti-MMP-9 and anti-TGF-β1 antibodies. The data were analyzed using the following tests: non-parametric Mann-Whitney, chi-square, Fisher's exact test and Spearman's correlation test (P<0.05). Analysis of inflammatory infiltrate revealed that 78% of periapical granulomas presented infiltrate grade III, in contrast with 32% of radicular cysts (P<0.001). Morphological evaluation of the epithelial thickness in radicular cysts revealed the presence of atrophic epithelium in 86% of the cysts. The immunostaining of MMP-9 was score 2 in 67% of the granulomas and 77% of the cysts. Both lesions were predominantly score 1 for TGF-β1. Significant differences were confirmed between the expression scores of TGF-β1 and MMP-9 in periapical granulomas (p = 0.004) and in radicular cysts (p < 0.001). Expression of TGF-β1 was different for periapical granulomas and radicular cysts. This immunoregulatory cytokine seems more representative in asymptomatic lesions. The extracellular matrix remodeling process dependent on MMP-9 seems to be similar for both periapical granulomas and radicular cysts. TGF-β1 and MMP-9 may play an important role in the maintenance of periapical lesions.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Declaration Of Interestmentioning

confidence: 99%

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Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions

et al. 2017

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“…Chronic PLs stand for granulomatous inflammation, where the process of exacerbation (symptomatic PLs) and their restriction (asymptomatic PLs) are present (2). It has been shown that the symptomatic PLs are characterized by the upregulation of adhesive molecules, chemotactic factors (3), T helper (Th)1 and Th17 cytokines as well as bone resorptive cytokines such as interleukin (IL)-1, IL-6, and tumour necrosis factor (TNF)-α (2,4). On the other hand, the generation of asymptomatic PLs is followed by the Th2 immune response, which promotes PL chronicity with restriction of inflammation (5).…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stem Cells from Periapical Lesions Upregulate the Production of Immunoregulatory Cytokines by Inflammatory Cells in Culture / Mezenhimske matične ćelije iz periapeksnih lezija stimulišu produkciju imunoregulacijskih citokina od strane inflamacijskih ćelija u kulturi

Marković¹,

Tomić²,

Djokić³

et al. 2015

Acta Facultatis Medicae Naissensis

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The pathophysiology of periapical lesions (PLs) is under control of pro-inflammatory and anti-inflammatory (mainly immunoregulatory) cytokines. We have recently established mesenchymal stem cells (MSCs) from PLs and showed their suppressive effects on the production of proinflammatory cytokines from PLs inflammatory cells (ICs). In this work we studied the production of interleukin (IL)-10, IL-27 and transforming growth factor (TGF)-β, by PL-ICs in direct or indirect contacts with PL-MSCs. PL-ICs, which were isolated from four different asymptomatic PLs, predominantly composed of lymphocytes, followed by neutrophil granulocytes, macrophages and plasma cells. PLMSCs, expressing typical MSC markers, were co-cultivated with PL-ICs at 1:10 ratio, either in direct contact or in a transwell-system, for 24 hours. The levels of cytokines in cell-culture supernatants were tested by ELISA. The results showed that PL-MSCs up-regulated the production of all three immunoregulatory cytokines by PL-ICs. PL-MSCs stimulated the production of IL-10 and IL-27 via soluble factors, whereas the up-regulation of TGF-β required direct cell-to-cell contacts. In conclusion, our results showed for the first time the involvement of PL-MSCs in restriction of inflammation in PLs by up-regulation of immunoregulatory cytokines.

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