Production of Probes for the Chestnut Genotypes by Rapd Markers

Typing European Chestnut (Castanea sativa Mill.) Cultivars Using Oak Simple Sequence Repeat Markers

“…The results obtained by indicate that SSR markers provide a powerful tool for the fi ngerprinting exhibiting advantages over RAPD and AFLP. The RAPD markers, used in chestnut by Fineschi et al (1993), , Paffetti et al (1999), and Santana et al (1999), are very sensitive to the PCR conditions and therefore are less reproducible in different laboratories. AFLP, that were used by Yamamoto et al (1998) for the genetic characterization of Asian species of Castanea, are extremely polymorphic but are based on a complex technique that requires high DNA quality.…”

“…Germplasm collection and characterization has been carried out in Spain, France, Italy and Greece and genetic studies on both cultivated and wild populations have been conducted by several authors (Fineschi et al, 1993;Paffetti et al, 1999;Pereira et al, 1999;Pereira-Lorenzo and Fernandez-Lopez, 1993;Pereira-Lorenzo et al, 1996;Santana et al, 1999;Villani et al, 1991Villani et al, , 1993 using isozyme, randomly amplifi ed polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers, with the aim of assessing genetic variability, constructing phylogenetic trees and fi ngerprinting cultivars. In addition, linkage maps for Castanea have been constructed (Barreneche et al, 2003;Casasoli et al, 2001;Kubisiak et al, 1997).…”