Production of pro- and anti-inflammatory cytokines by monocytes from patients with paracoccidioidomycosis

Peraçoli, Maria Terezinha Serrão; Kurokawa, Cilmery Suemi; Calvi, Sueli Aparecida; Mendes, Rinaldo Pôncio; Pereira, Paulo Câmara Marques; Marques, Sílvio Alencar; Soares, Ana Flávia

doi:10.1016/s1286-4579(03)00040-6

Cited by 79 publications

(59 citation statements)

References 35 publications

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“…Some reports have suggested that monocytes from PCM patients are the main source of IL-10 (2,33). In this study we demonstrated that monocytes challenged with high virulence P. brasiliensis strain induce higher levels of inflammatory and anti-inflammatory cytokines compared to low virulence strain.…”

Section: Monocyte Cytokines Induced By Paracoccidioides Brasiliensissupporting

confidence: 58%

“…In this study we demonstrated that monocytes challenged with high virulence P. brasiliensis strain induce higher levels of inflammatory and anti-inflammatory cytokines compared to low virulence strain. In a previous study we showed that monocytes from PCM patients produce IL-1β, IL-6, IL-8, TNF-α, and anti-inflammatory cytokines, IL-10 and TGF-β1 (33). These results suggest that P. brasiliensis antigens can induce systemic inflammatory response as observed in coccidioidomycosis (26).…”

Section: Monocyte Cytokines Induced By Paracoccidioides Brasiliensismentioning

confidence: 70%

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Pro‐ and Anti‐Inflammatory Cytokines Produced by Human Monocytes Challenged In Vitro with Paracoccidioides brasiliensis

Kurokawa

Araújo

Soares

et al. 2007

Microbiology and Immunology

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Monocytes and macrophages play a central role in innate and adaptive immune response against systemic fungal infections. Imbalances in suppressor or stimulatory cytokine secretion caused by these cells may influence disease development, microorganism death, and the nature of the adaptive immune response. This study analyzed the monocyte cytokine profiles of healthy individuals challenged with high and low virulent strains of P. brasiliensis and mRNA cytokine expression kinetics by reverse transcription polymerase chain reaction (RT‐PCR). Peripheral blood monocytes from healthy volunteers were cultured in vitro with and without virulent (Pb18) or low virulence (Pb265) strains from P. brasiliensis viable yeast cells. Interleukin‐1 beta (IL‐1β), IL‐6, IL‐8, IL‐10, tumor necrosis factor‐alpha (TNF‐α), and transforming growth factor‐beta (TGF‐β1) were measured in culture supernatants by enzyme immunoassay (ELISA), and mRNA cytokine expression was determined by RT‐PCR at 0, 4, 8, 12, 18 and 48 hr. Both P. brasiliensis strains induced monocyte production of IL‐1β, IL‐6, IL‐10 and TNF‐α. Pb18 induced higher levels of IL‐1β, IL‐6, and IL‐10 than Pb265. IL‐8 and TGF‐β1 levels were not significantly different from those cultured without stimulus. The mRNA cytokine expression was similar to supernatant cytokines measured by ELISA. In vitro monocyte challenge with virulent P. brasiliensis strain induces earlier and higher levels of pro‐ and anti‐inflammatory cytokines than low virulence strain.

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Section: Monocyte Cytokines Induced By Paracoccidioides Brasiliensissupporting

confidence: 58%

Section: Monocyte Cytokines Induced By Paracoccidioides Brasiliensismentioning

confidence: 70%

Pro‐ and Anti‐Inflammatory Cytokines Produced by Human Monocytes Challenged In Vitro with Paracoccidioides brasiliensis

Kurokawa

Araújo

Soares

et al. 2007

Microbiology and Immunology

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“…Several groups addressed this question by quantifying the cytokines released in vitro from patients' cells. The results published so far were, in general, quite concordant and the picture that emerged were quite consistent, as depicted in Table 2 [19,25,[27][28][29][30][31][32][33][34]. In brief, these studies showed that the acute/subacute form of the disease is associated with down modulation of the Th-1 cytokine pattern and expression of a dominant Th-2 immunity consisting of high levels of a subset of cytokines such as IL-4, IL-5 and IL-10, specific antibodies of the IgE and IgG4 subclasses, in situ and peripheral blood eosinophilia, among other parameters.…”

supporting

confidence: 64%

“…Their monocytes preferentially release IL-10 both without exogenous stimulation and when challenged in vitro with the P. brasiliensis main antigenic component, the 43 kDa glycoprotein, or with LPS [19,29,32]. Little or no IL-12p70 was released [Romano CC, Mendes-Giannini MJS, and Benard G, unpublished data].…”

mentioning

confidence: 99%

An overview of the immunopathology of human paracoccidioidomycosis

2008

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We review here the advances in the understanding of the immunopathology of human paracoccidioidomycosis (PCM). Its investigation must take in account the intriguing natural history of the mycosis and its agent, providing clues to the mechanisms that lead to development of disease (unbalanced host-parasite relationship?) or to the clinically silent, chronic carrier state (balanced hostparasite relationship?), in exposed people living in endemic areas. Although the literature on this subject has progressed notably, the overall picture of what are the mechanisms of susceptibility or resistance continues to be fragmentary. Major advances were seen in the description of both the cytokines/chemokines associated to the different outcomes of the hostparasite interaction, and the fungus-monocyte/macrophage interaction, and cytokines released thereof by these cells. However, relatively few studies have attempted to modify, even in vitro, the patients' unbalanced immune reactivity. Consequently, the benefits of this improved knowledge did not yet reach clinical practice. Fortunately, the previous notion of the immune system as having two nearly independent arms, the innate and adaptive immunities, leaving a large gap between them, is now being overcome.Immunologists are now trying to dissect the connections between these two arms. This will certainly lead to more productive results. Current investigations should address the innate immunity events that trigger the IL-12/IFN-c axis and confer protection against PCM in those individuals living in endemic areas, who have been infected, but did not develop the mycosis.

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“…Outros estudos corroboram com esta hipótese Del Sero verificou que camundongos IL-10 KO produzem espontaneamente ou quando infectados com C. albicans ou A. fulmigatus elevadas quantidades de IL-6 e dos isótipos IgG2a e IgG1 (DEL SERO et al, 1999). Além disso, em pacientes com paracoccidioidomicose (PCM) a produção de IL-6 endógena pode ocorrer devido a uma ação direta do fungo sobre os macrófagos, justificando os altos níveis de anticorpos encontrados nesses pacientes (PERAÇOLI et al, 2003).…”

Section: Discussionunclassified

A função da IL-10 na paracoccidioidomise pulmonar murina.

Costa¹

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LISTA DE ABREVIATURAS E SIGLAS Ac AnticorpoAg Antígeno APC Célula apresentadora de antígeno BHI Brain Heart Infusion CCR7CC chemokine receptor 7 CD11bMarcador de expressão celular para monócitos e células mielóides ou Mac-1 CD11cMarcador de expressão celular para monócitos e células mielóides ou CR4 CD4Marcador do linfócito Th1/Th2/Th17, co-receptor para molécula MHC classe II CD8Marcador do linfócito T citotóxico, co-receptor para molécula MHC classe I CD25Marcador de ativação de celular em linfócitos, cadeia α do receptor de IL-2 D.O.Densidade óptica E.P. Erro padrão ELISA Ensyme-linked immunosorbent assay FACS Fluorescence-activated cell sorting FITC Isotiocianato de fluoresceína FoxP3 Forkhead family of transcriptional regulator Foxp3FSC foward scatter, tamanho celular G-CSF Granulocyte colony-stimulating factor GITR Glucocorticoid-induced tumor necrosis factor receptor GM-CSF Granulocyte macrophage colony-stimulating factor H&E Hematoxilina e eosina HTT Hipersensibilidade do tipo tardio IA bMarcador de expressão de MHC classe II em monócitos IgG2aImunoglobulina G isótipo 2a IgG2bImunoglobulina G isótipo 2b IgG3Imunoglobulina G isótipo 3 IgG4Imunoglobulina G isótipo 4 IgM Imunoglobulina M IL-2Interleucina 2 IL-4Interleucina 4 IL-5Interleucina 5 IL-6Interleucina 6 IL-10Interleucina 10 IL-12Interleucina 12 IL-17Interleucina 17 i.p. Intraperitoneal i.t. Intratraqueal KO Knockout ou deficiente LPS Lipopolissacáride MCP-1Monocyte chemoattractant protein-1 MHC Major histocompatibility complex MØ Macrófago NK Natural killer NKT Natural killer T NO Nitric oxide OPD Ortofenilenodiamina Pb Paracoccidioides brasiliensis Pb 18Paracoccidioides brasiliensis isolado 18 P. brasiliensis Paracoccidioides brasiliensis PBSSolução salina em tampão fosfato PCM Paracoccidioidomicose PI Iodeto de propídio PMA Phobol 12-myristane 13-acetate PMN Polimorfonucleares RPMIMeio para cultura celular, tampão bicarbonato, pH 6,8

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Production of pro- and anti-inflammatory cytokines by monocytes from patients with paracoccidioidomycosis

Cited by 79 publications

References 35 publications

Pro‐ and Anti‐Inflammatory Cytokines Produced by Human Monocytes Challenged In Vitro with Paracoccidioides brasiliensis

Pro‐ and Anti‐Inflammatory Cytokines Produced by Human Monocytes Challenged In Vitro with Paracoccidioides brasiliensis

An overview of the immunopathology of human paracoccidioidomycosis

A função da IL-10 na paracoccidioidomise pulmonar murina.

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