Production of primate monoclonal antibodies

Review: Veterinary Sources of Nonrodent Monoclonal Antibodies: Interspecific and Intraspecific Hybridomas

“…Prefusion activation has also been used for horse, (56) cat and dog, (59,61) and sheep, (50) although only the latter study claim ed an improvement relative to unstim ulated PBL. Van Meurs and Jonker (31) found no improvement in the stabilization of chimpanzee hybridom as after stimulating the PBL prior to fusion with PWM or IL-2. Neidbala and Stott (106) found that anti-CD40 was not effective in stim ulating B cell blasts in hum an studies.…”

“…Primary heteromyelom as have also been used to generate chimpanzee MAbs by backfusing EBV-transformed lymphocytes with aminopterin-sen sitive mouse 3 human heteromyelomas. (6,87) Ehrlich et al (98) and Van Meurs and Jonker (31) fused chim panzee lymphocytes directly with a human 3 mouse heteromyelom as. The advantages apparent with the use of primary heteromyelom a fusion partners have been further investigated by the use of secondary, tertiary, or higher orders of derivatization.…”

“…(27) As potential substitutes for human antibodies, and as model system s for the development of hum an fusions, primates have also received much attention, and antibodies have been developed in chimpanzees, (28)(29)(30) baboons, (28) and several species of macaque monkey. (28,(31)(32)(33)(34) The motivation for developing rat MAbs, (35) and to some extent hamster MAbs, (3 6,37) has been primarily the search for antibodies to endogenous murine antigens or antigens that produce poor responses in mice. The production of MAbs from rabbits (38)(39)(40)(41) and guinea pigs (42 ,43) has proved to be considerably more difficult, and, although som e immunoglobu lin fragment and intact rabbit antibodies have been reported from interspecific fusions, a most significant advance has been the development of a rabbit plasmacytoma fusion partner.…”

Systematic review of chimpanzee use in monoclonal antibody research and drug development: 1981-2010

“…the numbers in the table suggest several observations: For example, experiments in the most recent decade (2001-10) generally used smaller numbers of chimpanzees than in the previ- observe infusion reactions following their administration (Van Meurs and Jonker, 1986;Jonker et al, 1988). In the 1990's, many published articles on mAb studies with chimpanzees continued to focus on properties of the serum and the immune response.…”

Characterization of a Macaque Anti-Rh17-Like Monoclonal Antibody

“…Since nonhuman primates are the animals taxonomically the closest to man, they become an alternative source of lymphocytes for the production of mAbs capable of recognizing intraspecies differences [8, 9, 10, 11]. We describe here a mAb produced by a primate-mouse hybridoma derived from lymphocytes of a crab-eating macaque (Macaca fascicularis) immunized with human and gorilla RBCs.…”