Production of potato breeding material using somatic hybrids between Solanum tuberosum L. dihaploids and the wild diploid species Solanum bulbocastanum Dunal. from Mexico

Yermishin, A. P.; Makhan’ko, O. V.; Воронкова, Е. В.

doi:10.1134/s1022795408050086

Cited by 7 publications

(7 citation statements)

References 10 publications

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“…This innovation has gotten a misfortune and restricted consideration. In the meantime, recent studies identified with the generation of physical hybrid breeds have uncovered the potential role of this strategy in enriching plant germplasm, and for the creation of novel cultivars (ANANTHAKRISHNAN et al, 2006;TROJAK-GOLUCH and BERBE' C, 2007;YERMISHIN et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv.

2019

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To isolate protoplast, a pre-treatment was completed with the order to reduce and identify the phenolic contents round the year to encourage the isolation of protoplasts. Protoplasts from in vivo mesophyll leaves of apple cultivar “Anna” was isolated from 15 days old leaves by plasmolying in medium containing 90 g L-1 mannitol for half hour, then 130 g L-1 mannitol for half hour. Then using enzymatic mixture involving (1.5% cellulase + 0.5% pectianase + 1.5% Macrozyme) Prior to isolation. Anyhow, diverse factors, for example, Osmotic pressure, incubation period, sieve pore size, centrifugation period and hormonal balance was estimated using the techniques for isolation. The quantity of cells was computed as the quantity of cells per square on haemocytometer. A considerable higher yield of protoplast formation was noted in the CPW medium using a pore size of 25 µm with using incubation period for 20 hours. Moreover, the best protoplast regeneration with using of protoplast density of 2.0 x 105 in MS medium supplemented by 1.0 mg L-1NAA and 0.3 mg L-1BAP. We believed that our protocol might encourage the plant recovery using in apple somatic hybridization programs.

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Section: Introductionmentioning

confidence: 99%

Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv.

2019

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“…At that, achievements in development and implementation of prezygotic incompatibility overcoming approaches, based on the use of segregation for S-genes of parental species, shall be noted. As a result, we involved the tetraploid somatic hybrids of S. tuberosum dihaploids with Mexican diploid wild species S. bulbocastanum and S. pinnatisectum in breeding program [9,10]. Additionally, we have shown that wild diploid potato species S. verrucosum and its laboratory SvSv-lines deficient on pistillate S-RNAses, could be successfully used for overcoming pre-and postzygotic incompatibility at interspecies crossing.…”

Section: E V E L O P M E N T O F P O T a T O D I H A P L O I D Smentioning

confidence: 99%

DEVELOPMENT OF INITIAL MATERIAL FOR MARKER ASSISTED POTATO (Solanum tuberosum L.) PARENTAL LINE BREEDING AT THE DIPLOID LEVEL (review)

Yermishin¹,

Воронкова²

2017

S-h. biol.

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A b s t r a c tIt is important for improving the efficacy of potato breeding to have parental lines that are multiplex for many genes of agronomic characters, first of all genes of resistance to pests and diseases (that have two or more dominant alleles in the locus) (J.E. Bradshaw et al., 1994). Parental lines that are multiplex for several resistance genes occur not often (A.P. Yermishin et al., 2016) and, owing to biological peculiarities of potato crop, they can be produced only by means of breeding at the diploid level. In the presented review the genetic principles are described and an experience of their use by authors in research devoted to the development of the initial material applicable for effective marker assisted potato parental line breeding at the diploid level. The material includes the collection of initial dihaploids originated from potato varieties which were selected for viability, tuber performance, cultivar characters and DNA-markers of pest and diseases resistance genes; the diploid breeding material having wide range of late blight and virus resistance genes, introgressed from wild potato species by means of original methods of overcoming interspecific reproductive barriers; the diploid lines -donors of self-incompatibility gene and high male fertility genes that are effective as in heterozygous as well as in homozygous condition; the diploid lines -donors of genes of male fertile FDR (first division restitution) 2n-gamete formation. The best diploid lines having a complex of pest and diseases resistance genes, selected as the result of diploid breeding (on the basis of initial dihaploids, interspecific hybrids and donors of fertility) were used for production of multiplex tetraploid parental lines by means of mitotic chromosome doubling. Diploid parental lines suitable for hybridization with potato varieties (for meiotic polyploidization) were bred using lines-donors of genes of male fertile FDR 2n-gamete formation. The diploid initial material developed as the result of the research is of the interest for prospective alternative directions of potato breeding using selection at the diploid level: breeding diploid potato varieties as well as parental lines for production of hybrids and hybrid populations for true potato seed technology.

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“…Для решения данной проблемы нами использован методический прием, основанный на получении тетраплоидных растений-регенерантов (андрогенных клонов, андроклонов) в культуре пыльников гибрида IGC 16/36.1 и последующих скрещиваний их с сортами культурного картофеля. Предполагается, что в процессе микроспорогенеза происходит рекомбинация генетических факторов, контролирующих EBN, и появляется вероятность выщепления среди андроклонов индивидуальных растений с эффективной плоидностью, близкой к 4 EBN и, благодаря этому, способных скрещиваться с сортами культурного картофеля (Yermishin et al, 2008).…”

Section: Introductionunclassified

“…Условия культивирования: температура 22 о С днем, 18 о С ночью. Описанная методика обеспечивает, как правило, получение андроклонов того же уровня плоидности, что и исходные растения (Yermishin et al, 2008). Важнейшим элементом технологии является выращивание растений-доноров пыльников с применением дополнительного освещения лампами дуговыми ртутными с иодидами металлов ДРИ-2000-6, что позволяет существенно повысить их способность к андрогенезу in vitro (Yermishin, 1998).…”

Section: Introductionunclassified

The use of in vitro androgenesis for the involvement of interspecific hybrids between Solanum tuberosum L. and wild allotetraploid potato species Solanum stoloniferum Schltdl. et Bouché into breeding

Yermishin,

Ageeva,

Voronkova

et al. 2024

Biotehnologiâ i selekciâ rastenij

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Wild allotetraploid potato species Solanum stoloniferum Schltdl. & Bouché from Mexico is regarded as a valuable source of resistance genes for use in breeding. However, introgression of its resistance genes into breeding material is hampered by a set of reproductive barriers. The genomic difference between S. stoloniferum (genome ААВВ) and S. tuberosum L. (AAAA) is one of them. This makes questionable the possibility of transferring a variety of valuable genes of the wild species localized on the chromosomes of its genome B into the genome of cultivated potatoes. It is proposed to produce tetraploid (4x, AAAB) interspecific hybrids of S. stoloniferum, which are regarded as more promising for homoeological recombination than pentaploid (5x, ААААВ) hybrids commonly used in the introgression schemes. However, the effective ploidy of tetraploid hybrids (3EBN) hinders their backcrossing to cultivated potatoes (4 EBN). For instance, our attempts to involve the tetraploid hybrid of S. stoloniferum IGC16/36.1 obtained by us into hybridization with potato varieties were unsuccessful for a number of years. To solve this problem, we suggested a technique based on the production of 4x plants obtained in anther culture of this hybrid. The present research was aimed at assessing the efficiency of this approach.Thirty-one plants were obtained in anther culture (androgenic clones, androclones) of the hybrid IGC16/36.1 in 2018. Most of them exceeded the initial hybrid in habitus strength and flowering intensity. As a result of crosses made in 2019, 1039 hybrid seeds were obtained from crossing 21 androclones with the ‘Lemhi Russet’ variety (8.7 seeds/pollination), 1017 seeds (7.5 seeds/pollination) from crosses of 23 androclones with the ‘Quarta’ variety, and 716 seeds (12.3 seeds/pollination) from crosses of 11 androclones and a diploid potato line IGC 17n8 capable of producing fertile unreduced (2n) pollen. The hybrid seeds had good germination rate of 70-90%. Among the androclones that gave progeny in crosses with potato varieties, we identified genotypes carrying DNA markers of late blight (LB) resistance genes Rpi-sto1, R2 and R3b, PVY resistance genes Ryadg, Rysto and Rychc, and potato wart disease resistance gene Sen2 (these markers were found in the initial accession of S. stoloniferum PI 205522 and in the IGC 16/36.1 hybrid). Despite the complex nature of inheritance of the analyzed markers in progenies of backcrosses of androclones, a number of isolated hybrids carried several markers, including those of the Rpi-sto1, a broad-spectrum gene for high resistance to late blight. Hybrids with relatively high tuber productivity, features of cultivated potatoes such as regularly shaped tubers with small eyes, and high field resistance to late blight were selected.The prospects for using androclones of the tetraploid interspecific hybrid IGC 16/36.1 for increasing the frequency of homoeologous A/B recombination of chromosomes are discussed.

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Production of potato breeding material using somatic hybrids between Solanum tuberosum L. dihaploids and the wild diploid species Solanum bulbocastanum Dunal. from Mexico

Cited by 7 publications

References 10 publications

Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv.

Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv.

DEVELOPMENT OF INITIAL MATERIAL FOR MARKER ASSISTED POTATO (Solanum tuberosum L.) PARENTAL LINE BREEDING AT THE DIPLOID LEVEL (review)

The use of <i>in vitro</i> androgenesis for the involvement of interspecific hybrids between <i>Solanum tuberosum</i> L. and wild allotetraploid potato species <i>Solanum stoloniferum</i> Schltdl. et Bouché into breeding

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